选择培养

复合菌群原液中含有不同种类的微生物，通过选择培养基的定向富集培养，可以得到不同目标优势菌群；

Many kinds of microorganisms exist in the original liquor of synthetic-bacteria , and through directive enriching cultivation with the selected culture medium , the superior synthetic-bacteria for different objectives can be obtained ;

该株细胞能抵抗8－AG的毒性作用，对HAT选择培养基十分敏感。

The mutant cells are resistant to cytotoxic effect of 8 AG and sensitive to hypoxanthine aminopterin thymidine ( HAT ) selective medium .

HPT转基因水稻对选择培养的品种间差异

Varietal difference of selective culture of HPT gene rice

ColiDH5αTM细胞内选择培养，以纯化质粒为模板，扩增出各基因座的等位基因片段后混合。

Coli DH5 α TM cells , worked on selective culture , and finally , mixed the alleles of the four STR loci respectively .

用选择培养基从污水处理厂污泥中筛选出能降解3种染料（OrangeⅠ，OrangeⅡ和酸性大红）的菌株。

The strains that can degrade three kinds of dyes ( Orange ⅰ, Orange ⅱ and Great Acid Red ) were screened from the sludge of wastewater treatment plant with a selected medium .

将亲本杂交瘤分别诱变为HGPRT~-及TK~-，融合后以HAT选择培养并辅以饲养细胞较易获得二次杂交瘤。

It was more successful to get hybrid-hybridomas , when the parental hybridomas were induced to HGPRT - and TK - mutants for fusion and feed cells were used in HAT selective cultures .

常规方法进行融合，HT、HAT培养液选择培养融合后的细胞，ELISA检测细胞培养上清中的LPS抗体，阳性杂交瘤细胞用有限稀释法克隆传代。

Conduct an enhancing immunization three days before Conventional methods of integration . Selectively cultivate fusion cells in HT and HAT medium . Cell culture supernatant is checked for LPS antibody using ELISA .

对不同来源菌株的细菌作分离及生化鉴定，确定了Ah的六项生化鉴定指标，同时比较确定了AHM和RS作为Ah的选择培养基。

Biochemical indexes were determined based on the isolation and biochemical identification of Ah strains of different origins . and AHM and RS were the optimal selective media for Ah .

在这两种细菌原生质体融合试验中，选择培养基中红霉素的应用浓度为3000Iu／ml，庆大霉素应用浓度为50Iu／ml。

In this protoplast fusion experiment between two bacteria , the applying density in selective culture medium of Erythomycin and Gentamicin are 3000 lu / ml and 50 lu / ml.

提高转化效率的关键在于降低共培养温度至19～22℃，并在选择培养10～15d后及时选择继代培养4～12d。利用上述程序构建了大规模的水稻增强子捕获突变体库。

Key factors for transformation efficiency improvement included decreasing co-culture temperature to 19-22 ℃, and subculture selection for 4 ~ 12d after selection culture for 10 ~ 15d .

待幼苗长到约2cm高的时候，将其转入生根选择培养基M4进行选择培养，部分幼苗能生根，并逐渐发育形成明显的根系，将幼苗移植到土中培养。

Tender plants were transferred to M4 medium when they were about 2 centimeters in height . Some plants , which rooted and formed obvious root system , were transplanted into soil .

〔结论〕卡那霉素ELB培养基是一种简便、有效的分离肠道肠球菌的选择培养基。

〔 Conclusion 〕 kanamycin ELB is a convenient and effective selective medium to isolate enterococci from fecal specimens .

应用Lipofectin方法先将嵌合轻链表达载体转染到Sp2/0细胞中，经用含霉酚酸的选择培养基筛选及克隆化培养，获得稳定分泌3H11人-鼠嵌合轻链的转染细胞株。

The chimeric light chain expression vector was first transfected into murine Sp2 / 0 myeloma cells using lipofectin . Transfectants that stably expressed human-mouse chimeric light chain were isolated by mycophenolic acid selection .

利用直接筛选的方法，将胚性愈伤组织分别放在含1%，2%和3%NaCl的选择培养基上连续筛选60d，在1%NaCl浓度下获得了耐盐的胚性愈伤组织并且获得了再生植株。

The embryogenic calli were selected on a medium containing 1.0 % ,( 2.0 % ,) 3.0 % NaCl and directly continued for a further 60 d. Salt-tolerant variant types of calli were obtained from the medium containing 1.0 % NaCl .

血消化液琼脂淋球菌选择培养基的实验研究

Experimental research on isolating gonococcus by blood-digested agar selective medium

鼠疫菌糖醇酵解鉴别培养基与选择培养基的研究

A Study of the Identified and Selective Plate for Glycolysis of Yersinia Pestis

嗜水气单胞菌选择培养基鉴别效果的比较

Comparison of the Selective Culture Mediums of Aeromonas Hydrophila

应用选择培养基，从鸡慢性呼吸道病的自然病例中分离鸡毒霉形体，在棉拭样本中得到四个霉形体分离物。

By using a selective medium , four mycoplasma isolates were obtained from swabs taken from the field cases of chronic respiratory disease in chickens .

本研究主要以西北几个地区葡萄根际土壤为试验材料，利用平板培养法和微生物选择培养基进行培养分离，初步筛选出具有溶有机磷能力的细菌。

The method of plate culture and selective medium were used to culture and separate soil microbes , and then organic-phosphorus solubilizing bacteria was screened preliminary .

一旦你选择培养一种新的习惯，你就需要创造一种方法来支持你的新习惯。

Once you pick the new habit you 're ready to adopt , next you 'll want to create a method that will support your new habit .

悬浮培养天数、悬浮培养基激素组成和选择培养基种类明显影响转化频率。

Several factors , such as culturing time of suspension cultures , phytohormone constitution of suspension medium and basal selection medium , affected the transformation frequency apparently .

结论：血消化液选择培养基分离淋球菌具有经济、简便、分离率高等优点，是一种较理想的培养基。

Conclusion : The results indicate that blood-digested culture medium has advantages of correctness , economy and simplicity and is a kind of ideal culture medium of isolating gonococcus .

利用选择培养基对复合菌群进行富集培养，可以得到不同优势菌为主的富集培养液，提高了微生物的代谢活性和对环境的适应性。

The enrichment incubation of complex bacterium by selecting culture medium could receive the enrichment incubation liquid with different dominant microbes and raise microbial metabolic activity and its adaptability to environment .

此外，通过提高选择培养中硝酸铵的浓度，可以提高香石竹的遗传转化率。

In addition , the impact of ammonium nitrate on the conversion rate is explored , and the rate of genetic transformation of carnation is increased through increasing the amount of ammonium nitrate .

本文报道应用选择培养基分离培养法，检测了202名正常人鼻腔中的葡萄球菌，并对其生物学性状及药敏性进行了分析。

Using high-salt broth combined with high-salt mannitol agar plate method , 84 strains of staphylococci were detected from the nasal cavities of 202 healthy persons , and their biological characteristics and drug-resistance were analyzed .

1993～1996年，在大豆苗期和成株期，对黑龙江省部分大豆产区的48个乡镇进行了调查。共采集435份标样，在选择培养基上分离，共得到18个疫霉菌株。

The incidence of phytophthora root rot in 48 villages and towns of soybean production in Heilongjiang province was investigated in 1993 ~ 1996 . 435 samples of the disease were collected and 18 isolates were obtained .

努力掌握和运用这一规律，并正确选择培养中青年骨干教职人员的有效路径，对于我国宗教界发展的未来具有重要的现实意义。

Trying hard to grasp and use this regularity , and accurately choose an efficient avenue for cultivating young skeleton teachers has a momentous practical meaning on the future of our country 's development in the religious field .

方法：将采集的25种样品，选择培养基Ⅰ连续传代培养，通过比较胆固醇降解率和酶活力，筛选出降解胆固醇能力较高的菌株，并研究其酶学特性。

Methods : The 25 kinds of food samples collected were continuously cultivated in selective medium ⅰ . The separated bacterium were screened through comparing their rates of cholesterol-degrading and enzyme activities , and enzymology characteristic was primarily studied .

从福建、河南、安徽、云南、湖北采集了烟草根际土样100份，用几丁质选择培养基法共分离出产几丁质酶菌株62株，其中真菌32株、细菌22株、放线菌8株。

100 samples of tobacco rhizosphere soil were collected from Fujian , Henan , Anhui , Yunnan and Hubei , 62 chitinase producing strains , including 32 fungi , 22 bacteria and 8 actinomyces , were isolated by chitin selective medium .

我们选择AS培养的hMSCs进行成骨诱导。

So we have chosen AS to conduct hMSCs into osteogenic culture . 2 .