基因传感器

压电基因传感器检测巨细胞病毒PCR产物的实验研究

Experimental study of detecting PCR product of cytomegalovirus with piezoelectric gene sensor

缓冲液pH值对肽核酸压电基因传感器阵列杂交效应的影响

Effects of pH values of buffer on hybridization efficiency with PNA piezoelectric gene sensor array

应用压电基因传感器芯片检测结核分枝杆菌DNA

Detection of Mycobacterium tuberculosis DNA by Piezoelectric Biosensor Chip

研究了适合构建压电基因传感器的、具有良好LM特异性的DNA检测探针，为研制检测LM的压电基因传感器技术提供了物质基础。

DNA probes with good LM specificity and fit for constructing QCM-DNA sensor were designed .

DNA生物传感器又称基因传感器是近年来发展最迅速的一类传感器，特别是在人类基因组图谱成功绘制出之后，基因研究越来越受到科学家们的关注。

With the development of genetics , DNA biosensor had gotten more and more attention . The studies on DNA become very important especially after the finish of genome .

SDA反应可引起压电基因传感器的频率持续下降；

SDA could lead to continuous reduction of frequency in piezoelectric gene sensor .

长、短链探针以链延伸反应提高基因传感器检测MRSA灵敏度的实验研究

Sensitivity Improvement of Detecting MRSA Using Gene Sensor by Strand Extension Reaction with Long and Short Chain Probes

实验温度是影响SDA压电基因传感器检测灵敏度的重要因素。

Test temperature is important to effect the sensitivity of the SDA piezoelectric gene sensor .

目的探索乙型肝炎病毒（HBV）靶序列浓度值对肽核酸压电基因传感器阵列杂交效应的影响。

OBJECTIVE To investigate the effects of hepatitis B virus ( HBV ) target concentration on hybridization efficiency of peptide nucleic acid ( PNA ) piezoelectric gene sensor array .

肽核酸和RecA蛋白&互补单链DNA探针生物信号放大系统用于压电基因传感器的实验研究

Experimental Study of Piezoelectric Gene Sensor by PNA and the System of Biology Magnified of " RecA Protein-complementary Single Strand DNA " Probe

目的应用自行研制的压电基因传感器检测巨细胞病毒临床标本的PCR产物，探讨压电传感器检测核酸反应的可行性以及各种影响因素。

Objective To detect the PCR product of human cytomegalovirus ( HCMV ) using piezoelectric gene sensor made by ourselves and to explore the possibility and effect of detecting DNA reaction with piezoelectric sensor .

乙型脑炎病毒靶序列浓度对LSAW基因传感器检测系统的影响

Concentrations of target sequence of Japanese encephalitis virus in LSAW gene sensor detection system

目的探索不同肽核酸（PNA）探针浓度对PNA石英谐振式基因传感器阵列杂交效应的影响。

Objective To investigate the effects of peptide nucleic acid ( PNA ) probe on hybridization efficiency with PNA quartz oscillation gene sensor array .

结论在反应体系中加入RecA蛋白-互补单链DNA探针，能有效地提高压电基因传感器的灵敏度，明显缩短反应体系时间。

Conclusion The sensitivity of piezoelectricity gene sensor array is improved and the time of procedure is decreased significantly by using the probe of RecA protein-complementary single strand DNA in reaction system .

结论成功构建压电基因传感器液相检测稳定平台并实现对SDA反应的实时检测；

Conclusion We have designed the system of piezoelectric gene sensor in liquid phase and conducted real time monitoring of the detection of SDA successfully .

目的在构建好的肽核酸压电基因传感器检测系统上引入RecA蛋白互补单链DNA探针复合体作为新型生物信号放大系统，提高传感器的检测灵敏度。

Objective To improve the detection sensitivity of peptide nucleic acid ( PNA ) piezoelectric gene sensor by introducing RecA protein-complementary single strand DNA probe complex as new style biological signal amplification system .

目的利用链延伸反应的原理延长引物链以提高石英谐振式基因传感器表面的质量负载，从而提高传感器检测MRSA的灵敏度。

OBJECTIVE To improve the sensitivity of detecting MRSA using quartz oscillation gene sensor ( QOGS ) by increasing its surface load on the basis of the principle of strand extension reaction .

方法基因传感器阵列固定上针对乙肝病毒的bisPNA探针，加入靶DNA与之反应，然后加入预先处理过的不同浓度（0.5～6mg/ml）的RecA蛋白互补单链DNA探针。

Methods Bis PNA probe for detecting HBV was immobilized on the surface of gene sensor array . Different concentrations of the " RecA protein complementary single strand DNA " probes were added after the bis PNA probes were hybridized with corresponding target DNA .

基因传感器阵列表面分别固定上金黄色葡萄球菌mecA及femA两种探针片段，待杂交上相应的靶序列片段后，加入Klenow酶在室温下进行链延伸反应。

Two kinds of mecA and femA probes of staphylococcus aureus were immobilized on the gold electrode surface of QOGS array . Klenow large fragment was added to extend the strand in room temperature after the probes were hybridized with corresponding target DNA . 4 .

离子浓度对肽核酸压电基因传感器杂交效应的影响

Effects of ion concentrations on hybridization efficiency with PNA piezoelectric gene sensor

离子强度对乙型脑炎病毒基因传感器检测系统的影响

Effects of Ionic Strength on Japanese Encephalitis Virus Gene Sensor Detection System

链延伸反应提高压电基因传感器的灵敏度的实验研究

Sensitivity improvement of piezoelectric gene sensor by strand extension reaction

单核苷酸多态性漏声表面波基因传感器检测系统的构建

Construction of Single Nucleotide Polymorphism Leaky Surface Acoustic Wave Gene Sensor Detection System

基因传感器表面两种探针固定法的比较

Comparison between 2 probe immobilization methods on the surfaces of piezoelectric gene sensors

一种新型肽核酸基因传感器阵列检测系统的构建

Construction of A New-style Detection System of Peptide Nucleic Acid Gene Sensors Array

压电基因传感器检测芽孢杆菌靶序列研究

Study on detection of Bacillus spp . target DNA sequences by piezoelectric gene sensor

石英谐振式基因传感器芯片表面探针修饰电极的构建

Construction of electrode modified with probe at the surface of quartz oscillation gene sensor chip

乙型肝炎病毒靶序列浓度对新型肽核酸压电基因传感器阵列的影响

Effects of Hepatitis B Virus Target Concentration on New Style Peptide Nucleic Acid Piezoelectric Gene Sensor Array

2种不同类型的核酸探针对压电基因传感器阵列检测特异性的影响

Influence of two different types of nucleic acid probes on detection for specificity of piezoelectric gene sensor array

结论在压电基因传感器探针固定中效果良好的巯基法在适配子探针固定中并不适用，生物素-亲和素法在适配子压电传感器探针固定中有明显优势。

Biotin-avidin method has a better ability to immobilize probe on the surfaces of aptamer-based piezoelectric quartz crystal sensor .