体外转录

依赖于DNA的RNA聚合酶与体外转录的研究Ⅰ.酵母变异株20B-12RNA聚合酶A和C的纯化及鉴定

Studies on DNA-dependent RNA polymerase and transcription in vitro I. purification and characterization of RNA polymerase A and C from mutant 20b-12 of Saccharomyces cerevisiae

这是首次有关负链RNA植物病毒遗传多样性的报道。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育2h。

After transcription in vitro , the PSTVd negative RNA was mixed with the ribozyme containing two or three cleavage sites separately and incubated two hours at 37 ℃ .

乙型肝炎病毒前C变异体的体外转录和翻译

Study on in vitro transcription and translation of the precore variant of hepatitis B virus

丁型肝炎病毒基因组RNA中核酶区的cDNA的体外转录

In Vitro Transcription of cDNA of Hepatitis Delta Virus Genomic RNA Ribozyme

大鼠肝细胞核游离型RNA聚合酶的体外转录活性测定

Determination of transcription activity of rat liver nucleic free RNA Polymerase in vitro

锤头状核酶RCP对HBV的P基因体外转录物的作用

Effect of hammerhead ribozyme ( RCP ) on HBV P gene in vitro

利用分子生物学技术制备了重组质粒、体外转录RNA，通过鉴定后建立了相应的重组质粒DNA阳性参比品、体外转录RNA阳性参比品。

Recombinant plasmid and in vitro transcription RNA were prepared and identified through molecular biology techniques .

精脒对E.ColiRNA聚合酶体外转录调节作用的研究

The Regulation of E.coli RNA Polymerase Transcription in Vitro by Spermidine

人IL-2受体α链cDNA体外转录产物的表达

Expression of human IL-2 receptor chain cDNA in vitro transcripts

同时构建前导RNA的转录模板，经体外转录得到地高辛标记的RNA。

Meanwhile , the DIG labeled RNA leader sequences were synthesized by in vitro transcription .

RNA干扰用siRNA的体外转录合成

Synthesis of siRNA by in vitro for Transcription RNA Interference

体外转录生成核酶和靶RNA分子，进行切割反应后经变性聚丙烯酰胺凝胶电泳，银染法显色判定结果。

After in vitro cleaving reactions polyacrylamide gel electrophoresis was carried out followed by the silver nitrate staining .

HCV全长cDNA细胞培养适应性突变体的构建及其体外转录制备感染性RNA的研究

Construction of Full-length HCV cDNA with Cell Culture-adaptive Mutations and Preparation of Infectious RNA by in Vitro Transcription

模板甲基化水平对鼠肝RNA聚合酶体外转录活性的影响

The effect of methylation level of DNA templates on the transcriptional activity of RNA polymerase from rat liver in vitro

经体外转录出核酶RZ1及靶RNA后，体外切割反应1小时，79.3%的靶RNA即被切开。

After in vitro transcription , the cleavage reaction was shown to have cut off 79.3 % target RNA in 1h .

T7RNA聚合酶体外转录合成siRNA的方法

A method of siRNA synthesis by T7 RNA polymerase transcription

方法采用基因克隆、基因重组、体外转录、RT-PCR技术，建立能同时检测细胞中4种热休克基因的RT-PCR体系，并将此体系用于检测SW13细胞中热休克基因的表达。

Methods RT-PCR system was established with gene cloning , gene recombination , in vitro transcription and RT-PCR techniques ;

目的：体外转录合成银松素合酶（PS）基因地高辛标记反义RNA探针。

Objective : To synthesize DIG - labelled pinosylvin synthase ( PS ) gene RNA anti-sense probe by in vitro transcription .

E.coli中alKA基因突变启动子及体外转录的研究

The promoter mutants and in vitro transcription of alka gene E.coli

方法：采用DNA顺序测定和体外转录的方法，以lac基因启动子为对照进行实验。

Methods : DNA sequencing and in vitro transcription had been used and the promoter of lac gene had been used as the control .

目的：建立一种应用T7RNA聚合酶体外转录合成大量siRNA的方法。

Objective : To establish a method using T7 RNA polymerase for obtaining large amount of siRNA .

二甲亚砜之前有报道称可以帮助以染色质或超螺旋结构的质粒为模板的RNA的体外转录。它的机制被认为是二甲亚砜改变了DNA模板的结构。

Dimethylsulfoxide was previously reported to facilitate in vitro transcription of RNA from chromatin and supercoiled plasmid , with the underlying mechanism being attributed to the alteration of the template structure .

采用体外转录地高辛标记的RNA探针，检测已知基因MDM2在鼠胚胎发育不同阶段的表达模式。

Here we take the MDM2 RNA probe labeled by DIG-11-UTP to detect the expression pattern in different stages of mouse embryos .

样品DNA通过PCR扩增、体外转录等技术掺入荧光标记分子，与微阵列杂交后，通过荧光扫描仪扫描及计算机分析即可获得样品中大量基因序列及表达信息。

After hybridization of the DNA chip with the labeled sample targets , high-throughput gene sequence and expression information can be retrieved by scanning of the DNA microarray and the analysis of computer .

以α-~3~2P-ATP为标记物研究了草鱼出血病病毒的体外转录，证实该病毒的核心具有RNA转录酶。

An RNA transcriptase activity of grass carp hemorrhage virus cores was demonstrated by the study on the in vitro transcription with α - 32P ATP as the label .

利用克隆获得的cDNA，进行体外转录，获得地高辛标记的cRNA探针。

The digoxin labeled cRNA probe was synthesised by in vitro transcription with the cloned cDNA as template .

方法：大鼠Caspase-3基因的PCR片段克隆于T载体T7启动子下游，32P标记的体外转录物作为靶RNA。

Methods : Rat caspase-3 gene fragment was cloned into T-vector under the control of T7 promoter . 32P-labeled caspase-3 transcript was target-RNA .

本研究建立的检测方法以体外转录的dsRNA为阳性对照，具有高度的稳定性，解决了类似检测方法中RNA阳性对照易降解的难题。

The RNA positive control in the assay was invitro transcribed dsRNA which was stabler than single strand RNA used in other similar test .

体外转录制备的siRNAs对SARS-CoV复制的抑制作用

Inhibition of SARS-CoVs replication by in vitro-prepared siRNAs

Northern杂交结果表明，包括核酶在内的3′末端非病毒序列在体外转录反应开始后就被顺式发夹核酶切除了。

Northern blot analysis indicates that the 3 ′ terminal non-viral sequence including the ribozyme had been cleaved by the cis - hairpin ribozyme from the in vitro transcripts soon after the in vitro transcription reaction .