原核表达

本研究首次将DH基因整合入原核表达载体中表达，为大量获取DH重组蛋白，研究DH的结构及功能奠定了基础。

We integrated DH gene into procaryotic expression vector for the first time , which establish foundation for obtaining DH recombinant protein to research the structure and function of DH .

结果测序结果表明，成功地将弓形虫主要表面抗原1单链抗体S1与爪蟾抗菌肽的融合基因构建到原核表达载体pET-32c中；

Results The fused gene of S1 and magainin was successfully cloned into procaryotic expression vector pET-32c proved by DNA sequencing .

人参CLASSⅢ几丁质酶基因克隆、序列分析及原核表达

Cloning , Sequence Analysis and Prokaryotic Expression of CLASS ⅲ Chitinase Gene from Panax Ginseng

中华鲟半胱氨酸蛋白酶抑制剂C基因的原核表达

Expression of Acipenser sinensis cystatin C in prokaryote

水疱性口炎病毒N基因原核表达载体的构建及表达

Construction and expression of vesicular stomatitis virus N gene in prokaryotic vector

人氨肽酶N基因克隆和原核表达

Molecular cloning and prokaryotic expression of human aminopeptidase N

目的：在原核表达系统中对人Era基因进行表达。

Objective : To study the expression of human Era .

小反刍兽疫病毒H基因的原核表达与鉴定

Prokaryotic expression of peste des petits ruminants virus H gene

苦荞过敏蛋白C端结构域的原核表达及其免疫学活性的鉴定

Prokaryotic Expression and Immunological Activity Identification of C-terminal Domain of the Allergenic Protein from Tartary Buckwheat

幽门螺杆菌鞭毛蛋白B亚单位原核表达系统的构建及其产物的免疫性

Construction of Prokaryotic Expression System of Helicobacter pylori Flagellin Subunit B Gene and Immunological Study of Expressed Product

乙型肝炎病毒全X基因的克隆及原核表达

Cloning and prokaryotic expression of HBV Whole-X gene

小反刍兽疫病毒H糖蛋白基因原核表达载体的构建及表达

Construction of prokaryotic vector and expression of H glycoprotein gene of peste des petits ruminants virus

犬瘟热病毒水貂分离株F基因的克隆及原核表达

Cloning and prokaryotic expression of F gene of canine distemper virus from ferret

乙型脑炎病毒贵州分离株E基因原核表达的研究

The Study of Prokaryotic Expression of Japanese Encephalitis Virus ( JEV ) isolated Strain E Genes

桃叶片β-半乳糖苷酶基因全长cDNA克隆及原核表达

Cloning and Expression of β - galactosidase Gene cDNA in Peach

伪狂犬病病毒gE基因的原核表达及间接ELISA方法的建立

Prokaryotic Expression of PRV gE Gene and Establishment of Indirect ELISA

将CP基因插入原核表达载体pBAD/HisC中，转化E。

The CP gene was inserted into pBAD / His C vector and expressed in E.

牛卵泡抑素cDNA的克隆及其原核表达

Cloning and Prokaryotic Expression of the Bovine Follistatin cDNA Gene

小鼠脑14-3-3蛋白ζ亚型cDNA克隆及其原核表达分析

CDNA cloning and prokaryotic expression of mouse brain 14-3-3 ζ isoform protein

彩鲫ran基因原核表达蛋白多克隆抗体的制备

Preparation of Multiclonal Antibody against Expressed Protein of Color Crucian Carp ran Gene in E.coli

方法：采用PCR、序列测定、原核表达及亲和层析法。

Methods : PCR , molecular cloning , prokaryotic expressing system and affinity chromatography were used .

甘蔗花叶病毒E株系CP基因原核表达与抗体制备

Prokaryotic Expression of CP Gene of Sugarcane Mosaic Virus Strain E and Preparation of Antibody

火菇素cDNA基因的原核表达与重组蛋白的纯化及活性研究

Prokaryotic Expression of Flammulin cDNA Gene and Purification of Recombinant Protein and Activity Research

裂谷热病毒Real-timePcR检测方法的建立及其G2部分蛋白的原核表达

Development of Real-time PCR Assay for Detecting RVFV and Expression of G2 Partial Protein by E.coli

肾上腺脑白质营养不良蛋白ATP结合区的原核表达与鉴定

Prokaryotic Expression and Characterization of ATP - binding Domain of X-linked Adrenoleukodystrophy Protein

丙型肝炎病毒依赖于RNA的RNA聚合酶基因克隆与原核表达

Cloning of RNA-dependent RNA polymerase ( RdRp ) gene of HCV and its expression in E.coli system

大豆花叶病毒CP基因原核表达与抗血清制备

Prokaryotic Expression of SMV CP Gene and Preparation of the Antibody Against CP

人血小板膜糖蛋白VI胞外区片段的原核表达及多克隆抗体的制备

Prokaryotic expression and polyclonal antibody preparation of human platelet glycoprotein ⅵ extracellular domain

HSV-1包膜糖蛋白G原核表达克隆的构建和表达

Construction and expression of the prokaryotic expression clone of HSV-1 envelope glycoprotein G

粉尘螨Ⅱ类抗原cDNA原核表达质粒的构建与表达

Construction and expression of prokaryotic expression plasmid of cDNA coding for group ⅱ allergen of Dermatophagoides farinae