原代细胞

〔方法〕采用原代细胞培养技术，研究18例HCC对ADM和CDDP的敏感性。

[ Method ] Sensitivities of ADM and CDDP on HCC were studied by primary cell culture .

方法NB患儿新鲜手术标本，采用组织块培养法、酶消化培养法建立原代细胞系；

Methods The neuroblastoma specimen was obtained from surgery and the primary cell lines were established by tissue mass culture and enzyme digestion culture respectively .

取大鼠膝关节滑膜组织体外进行原代细胞培养，用MTT比色法检测大鼠滑膜细胞的增生。

The knee synovial tissues of the rats were taken and synoviocytes were separated and cultured in vitro .

人骨肉瘤原代细胞条件培养基中骨形态蛋白（BMP）的实验研究

Experimental Study of Bone Morphogenetic Protein ( BMP ) in Conditioned Medium of Human Osteosarcoma Cell

共制备了3批冷冻细胞，每批均包括原质细胞、原代细胞和次代细胞，并将其分别放入I号冷冻液和II号冷冻液中冻存。

Three batches of cells , which were treated cell , original generation cell and second generation cell , were preserved in solution ⅰ and solution ⅱ .

结果表明，①药敏试验结果与卵巢癌细胞株的病理类型、原代细胞取材部位无肯定关联，但各卵巢癌细胞株对化疗药物的敏感性有显著性差异（P＜0－001）；

Results showed that ① Chemosensitivity had no positive relationship with the pathological classifications and primary focus sites . But , for different cell lines , the difference of chemosensitivity was significant ( P < 0.001 );

方法用小鼠骨髓嗜多染红细胞（PCE）微核试验和大白鼠肝原代细胞非程序DNA合成（UnscheduledDNASynthesis，UDS）实验检测冬凌草甲素对哺乳动物细胞DNA损伤的影响。

Methods Micronucleus assay of polychromatic erythrocytes in mice bone marrow and unscheduled DNA synthesis ( UDS ) in primary cells of liver in rat were used .

Citicoline对大鼠中脑原代细胞培养的神经保护作用

Neuroprotective effects of citicoline on 6-hydroxydopamine-treated mesencephalic dopaminergic neurons in primary culture

【方法】利用手术切除的人翼状胬肉组织进行原代细胞培养，即得到HPF。

[ Method ] HPF used were primary cell culture with exairesis human pterygium tissues .

有学者在体外培养原代细胞的基础上，诱导其自发性永生化或转染永生化基因（如SV40大T抗原和HPV18E6/E7等DNA肿瘤病毒），建立了永生化细胞系。

Several immortalized cell lines have been established either spontaneously or via gene ( including SV-40 large T antigen and HPV 18 E6 / E7 ) transduction .

方法：对4例脑胶质瘤病人的手术标本进行原代细胞培养，分不同时相收获上清，并对其上清进行IL-6活性检测。

Method : The human glioma cells ( HGC ) were isolated from fresh tumor specimen and cultured for different periods then assayed for interleukin-6 ( IL-6 ) activity .

结果细胞培养48h左右即开始贴壁，形态多呈梭形，原代细胞呈巢状生长。

Results Separated cells began to attach the wall around 48h , most morphology was spindle , primary cells were nested growth .

本研究旨在探讨凋亡存活蛋白survivin在白血病的原代细胞和白血病细胞系中的表达情况，并观察粒-巨噬细胞细胞集落刺激因子（GM-CSF）对其的影响。

This study was aimed to explore the expression of survivin in leukemia cells and to investigate the effect of GM-CSF on survivin expression .

猪细小病毒（PPV）强毒，在1～5日龄仔猪肾原代细胞上传代，传至20代左右毒力下降。

Porcine Parvovirus virulence was decreased when it was passed 20 generations in 1 ~ 5 day porcine kidney primary cell .

hTR反义寡核苷酸对急性白血病原代细胞端粒酶活性及凋亡的影响

The effect of human telomerase RNA ( hTR ) antisense oligodeoxynucleotides on telomerase acitvity and apoptosis in primary acute leukemia cells

Chk1/2反义寡核苷酸对DDP诱导白血病原代细胞凋亡影响的研究

Study of influence of antisense oligonucleotide targeting Chk1 / 2 on apoptosis of bone marrow mononucleate cells ( BMMNC ) of leukemia induced by DDP

依据贴壁培养法分离和扩增成骨细胞，噻唑蓝比色法（MTT）法检测原代细胞细胞增殖率绘制细胞生长曲线，取前4-6代细胞作为研究对象。

Adherent culture based on isolation and amplification of osteoblast , detected the cell proliferation rate by MTT assay and draw growth curve.4-6 generation of cells were taken into study . 2 .

方法用猪苓多糖、VitD3或二者联合对HL－60、K－562及白血病原代细胞进行体外诱导分化试验。

Methods PS-ZL and Vit D 3 were used alone or in combination to induce differentiation of HL-60 and K-562 cell lines and acute leukemia cells in vitro .

结果人乳腺癌原代细胞中的MDR1表达主要见于乳腺癌干细胞；

Results The expression of MDR1 in human breast cancer primary cells was mainly detected in breast cancer stem cells .

单抗标记FITC后用于人用鼠源性单抗制品及各种传代细胞和原代细胞中MAd检测，获得良好的实验结果。

Satisfactory results were obtained by using McAb-FITC for the detection of MAd virus potentially contaminated in primary and cultured cells and McAb of murine origin for human use .

家鼠型EHFV台（41）株在沙鼠肾原代细胞上产生血凝素的研究

Study of Production of Hemoagglutinin in Meriones Unguiculatus Kidney Primary Cells of House-rat Type EHFV T_ ( 41 ) Strain

一般于接种后2-4h原代细胞开始贴壁，48h后大部分原代细胞贴壁。

In general , 2-4h after inoculation of primary cells became adherent , 48h after most primary cell wall .

两个种属的大鼠均能获得ADSCs，原代细胞和传至4代细胞在倒置显微镜下观察，形态无明显差异。

Both of two kind rats can get ADSCs . No obvious difference between the original generation cells and 4th generation cells in microscope observation .

结果：原代细胞培养第18d细胞融合成单层，长满整个培养皿；

Primary prevention . Results : The primary cells formed a monolayer in 18th day .

光学显微镜下观察，原代细胞刚接种时悬浮于DMEM液体培养基中，呈圆形，细胞核位于胞浆中央。

Observation of optical microscope , primary cell Gang inoculation suspended in a DMEM liquid culture medium , the nucleus was round , located in cytoplasm of central .

方法取得不同患者的乳腺癌组织标本，采用胶原酶消化法获取乳腺癌原代细胞，应用原代培养和MTT法检测各种化疗药物在体外对乳腺癌原代细胞的杀伤效果。

Methods To receive breast cancer tissue from diverse patients by surgery , then to obtain mammary cancer cells by collagenase , the antitumor effect of chemotherapy drug with breast cancer cells was measured by culture in vitro and MTT .

采用组织块贴壁法、机械消化法分别培养雪旺细胞及神经元原代细胞，内皮细胞采用细胞株，并用S-100、β-tubulin-Ⅲ、vWF分别对其进行免疫学鉴定。

Schwann cells and neurons were cultivated by tissue block stick mechanical digestion method . Using S-100 , beta tubulin - ⅲ and vWF to immunofluorescence identify schwann cells , neurons , endothelial cells respectively .

贴壁原代细胞以分散的集落克隆方式迅速增殖，1W左右每个集落约含有数百个细胞，细胞形态基本为成纤维细胞样的长梭形。

Adherent primary cells to disperse the colony clonal rapid proliferation , 1W around each colony contains about hundreds of cells , cell morphology was long fusiform fibroblast-like .

方法采用MTT实验、硝基四氮唑蓝（NBT）还原实验和DNA凝胶电泳的方法，观察5AzaCdR对白血病细胞株和原代细胞增殖、分化和凋亡的影响；

Methods By using MTT test , NBT reduction reaction and DNA agarose gel electrophoresis , changes in proliferation , differentiation and apoptosis were observed in K562 , HL-60 and fresh leukemia cells after treated with 5-aza-CdR .

目的：探索人胃癌原代细胞在体外去除甲硫氨酸（Met）但含同型半胱氨酸（Hcy）的培养基（Met-Hcy+）中能否正常生长和去Met状态是否增加化疗药物对胃癌细胞的杀伤作用。

Objectives : To study the methionine dependence ( Met dependence ) of human primary gastric cancer cells in vitro when Met in the culture medium was replaced by its precursor homocysteine ( Hcy ), and the effect of methionine starvation in combination with chemotherapeutical agents on gastric cancer cells .