原胶原

原胶原mRNA水平用RTPCR法测定。

Type I procollagen mRNA level was determined by reverse transcription polymerase chain reaction ( RT-PCR ) .

目的：研究苦参素对成纤维细胞增殖及Ⅲ型原胶原mRNA表达的影响，探讨其抗纤维化的作用机制。

Objective : To study the effect of oxymatrine on fibroblastic proliferation and procollagen mRNA expression in search of effective medicine for liver fibrosis .

目的研究伤口愈合时成纤维细胞与纤维粘连蛋白黏附，以及黏附诱导的酪氨酸磷酸化蛋白在成纤维细胞表达原胶原mRNA中的作用。

Objective To investigate the role of adhesion between fibronectin and fibroblasts in wound healing as well as tyrosine phosphorylation proteins in procollagen mRNA expression .

整合素α5或β1亚基表达减少的成纤维细胞，原胶原proα1（Ⅰ）、proα1（Ⅲ）mRNA均显著降低。

Decreased expression of fibroblast integrin α 5 or β 1 subunit significantly lowered mRNA of procollagen α 1 (ⅰ) and α 1 (ⅲ) .

方法：用MTT法及Northern杂交分别检测NIH3T3成纤维细胞增殖及Ⅲ型原胶原mRNA的表达。

Methods : The proliferation and procollagen mRNA expression of fibroblast cell was studied with MTT bioassay and Northern blot hybridization .

经酪氨酸激酶抑制剂作用后，伴随着相对分子质量为98000、65000酪氨酸磷酸化蛋白的减少，原胶原proα1（Ⅰ）mRNA显著降低。

When the phosphorylation of tyrosine was blocked , the level of procollagen α 1 ( I ) mRNA decreased significantly with the reduction of 98 000 , 65 000 tyrosine phosphorylated proteins .

目的观察bFGF对人皮肤成纤维细胞增殖的影响及调控α1（I）原胶原基因序列的作用。

Objective To investigate the modulating effect of bFGF on the proliferation of human cutaneous fibroblasts and on the activity of human α 1 (ⅰ) procollagen gene promoter .

方法运用硫代修饰的整合素反义寡核苷酸阻断硬皮病成纤维细胞表面相应整合素亚基的表达，RTPCR检测整合素表达抑制后硬皮病成纤维细胞原胶原mRNA量的变化。

Methods Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin α 5 or β 1 subunit on scleroderma fibroblasts respectively , then the changes of procollagen mRNA were detected by RT PCR .

NaCl和TSPP处理的样品，胶原纤维分散成更细的原胶原纤维，好像散乱的丝线，且彼此很分散。

The collagen fibrils of NaCl and STPP treatments disassociated more thinner tropocollagen fibrils which liked disordered silk thread and separated from each other .

目的：研究金雀异黄素和槲皮素对HSC-T6大鼠肝星状细胞增殖和胶原合成及Ⅰ型原胶原mRNA表达的影响。

AIM : To study the effects of genistein ( GE ) and quercetin ( QU ) on proliferation , collagen synthesis , and procollagen messenger RNA ( mRNA ) expression of rat hepatic stellate cell line HSC-T6 cells .

结论体外培养人成骨细胞可合成原胶原，PICP分泌随增龄而降低，不同年龄组人成骨细胞均表达COL-1mRNA，30~40岁后表达量随年龄增长而降低。

PICP secretion was decreased with donor aging . All the human osteoblasts from different age donors cultured in vitro might express COL-1 mRNA . The quantity of COL-1 mRNA expression in the osteoblasts is decreased with donor aging .

小鼠α2（Ⅰ）型原胶原基因启动子活性研究

Activity analysis of promoter from mouse α 2 (ⅰ) procollagen gene

整合素表达对伤口愈合时成纤维细胞合成原胶原的影响

Effect of integrin expression on procollagen synthesis of fibroblasts in wound healing

原胶原分子间的交联结构拉伸力学特性的量子化学计算模拟

Quantum mechanics modeling of cross-link between collagen molecules

酪氨酸磷酸化蛋白在伤口愈合原胶原基因表达中的作用

The role of tyrosine phosphorylation proteins in procollagen gene expression of fibroblasts in wound healing

目的研究硬皮病成纤维细胞整合素表达对成纤维细胞合成原胶原的影响。

Objective Investigation of the effect of integrin on fibroblasts from scleroderma in the production of procollagen .

结果成骨细胞经体外诱导培养后透射电镜下观察，细胞外基质中可见大量原胶原；

Results Much tropocollagen could be found in the extracellular matrix by transmission electron microscope when the osteoblasts were cultured with ascorbate and β - glycerophosphate .

虽然骨形态发生蛋白13具有近似骨形态发生蛋白12的作用，能促进成腱细胞的增殖和Ⅰ型原胶原的基因表达，但对Ⅲ型原胶原的基因表达作用不显著。

Although bone morphogenetic protein-13 has the same biological effect to bone morphogenetic protein-12 , which can increase proliferation of tendon fibroblasts and the gene expression of procollagen type I , it has not obvious effect on the expression of procollagen type ⅲ .

纤维蛋白原鄄胶原海绵片与切口粘附紧；

Fibrinogen plus collagen sponge was adhered to the incision closely .

羊驼皮肤结构基因（原）胶原的表达特点

Expression of the Structure Gene ( Pro ) collagen of Alpaca Skin

实验组用纤维蛋白原鄄胶原海绵片进行止血，对照组用明胶海绵片止血。

Hemostasis was performed with fibrinogen plus collagen sponge in experimental group . Hemostasis was performed gelatin sponge in control group .

纤维蛋白原-胶原海绵片在肝手术创面的止血及组织封闭作用的动物实验研究

Experimental study on the hemostatic and sealing effects of fibrinogen plus collagen sponge using in the surface of hepatic operative wound

结论纤维蛋白原鄄胶原海绵片能有效地止血，与创面的粘附力强，吸收降解快，且能诱导肝创面肝细胞再生。

Conclusion Fibrinogen plus collagen sponge can effect to hemostasis . It is closely adhered to surface of wound and is absorbed quickly , and that it can induce regeneration of hepatic cells .

在大鼠左肝前叶切除术中，纤维蛋白原鄄胶原海绵片组在止血时间及出血量的指标上均优于明胶海绵片组（P<0.05）。

In the hemostatic time and hemorrhagic volume , the fibrinogen plus collagen sponge group was better than that the gelatin sponge group during hepatic resection of white rats ( P < 0.05 ) .

目的探讨富里酸对NMRI小鼠皮肤、肋骨及骨胶原合成及胶原分子中羟赖氨酸糖基化程度的影响。

Objective To understand the impact of fulvic acid ( FA ) on the hydroxylysyl glycosylation in collagen bio-synthesis .

背景：传统同种或异种生物瓣的表面缺乏宿主内皮细胞（EC）覆盖及基质缺乏间质细胞，是瓣叶钙化和原发性胶原退变的主要原因之一。

Background : One of the main causes of calcification and collagen fibrous degeneration is that the surface of the bioprostheses ( homografts and heterografts ) is lack of endothelial cells ( ECs ) and the matrix is lack of interstitial cells .

出于此目的，本论文展开了以下研究：1.以青鱼为原材料研究了胶原的提取、表征和应用。

For this purpose , this paper studies on the following : 1 . Extraction technology of collagen .

重组人骨形态发生蛋白12不但能使腱纤维原细胞的增殖，还能使Ⅰ型和Ⅲ型原胶原高表达，有助于肌腱的修复和愈合。

Recombinant human bone morphogenetic protein-12 can increase proliferation of tendon fibroblasts and the gene expressions of procollagen type ⅰ and type ⅲ, which is helpful to the repairing and healing of tendon tissues .