原代培养

大鼠垂体细胞的无血清原代培养

Primary Culture of Rat Pituitary Cells in Serum - free Medium

恶性肿瘤细胞体外原代培养与药敏试验

Primary culture of malignant tumour cells and drug sensitivity tests

蛋白激酶C亚型在原代培养神经元生长中的作用

Effects of protein kinase C subtype in the growth of primary cultured neurons

半定量RT-PCR法检测原代培养脂肪细胞生脂基因mRNA转录表达

Semi-quantitative RT-PCR method to measure mRNA expression for lipogenic genes in primary culture adipocytes

口腔癌原代培养细胞MTT法药敏试验方法的建立

Establishment of chemosensitivity testing of oral cancer using the MTT assay

肺癌细胞原代培养MTT法药敏试验研究

Study of drug sensitivity of lung cancer cells with MTT

Wistar大鼠晶体上皮细胞原代培养及鉴定

Primary culture and identification of wistar rat lens epithelial cells

SD大鼠星形胶质细胞的原代培养

Primary cultivation of SD rat cerebral astrocyte

方法将原代培养的神经干细胞移植入AD大鼠脑内，通过水迷宫和跳台实验观察AD大鼠的学习记忆能力；

Methods After the Alzheimer model was reproduced , NSCs were primary cultured and identified from hippocampus of rat embryo .

谷氨酸对原代培养的SD大鼠皮层神经元毒性作用量效关系研究

Study on dose-effect relationship of toxicity effect of glutamate on cortical neuron in primary cultured SD rats

阳离子多聚物jetPEI~（TM）对原代培养的猪血管平滑肌细胞的转染效率研究

Study of transfection efficiency of cationic polymer PEI to primary culture vascular smooth muscle cells of porcine

维甲酸对高氧暴露下原代培养的胎鼠肺泡II型上皮细胞和成纤维细胞增殖与凋亡的影响

Effect of Retinoic Acid on the Primary Rat Embryonic Type II Alveolar Epithelial Cell and Lung Fibroblasts Proliferation and Apoptosis Exposed to Hyperoxia

神经干细胞原代培养及GABA能神经元的诱导分化

Primary culture of neural stem cells and induction and differentiation of GABA neurons

方法原代培养新生大鼠颅盖骨成骨细胞，3H胸腺嘧啶掺入法测定成骨细胞的增殖；

METHODS Osteoblastic cells were isolated from newborn rat calvaria through trypsin digestion .

原代培养破骨细胞接种在材料表面并培养4h和48h。

Primary cultured osteoclasts were incubated for 4h and 48h on samples .

经原代培养及传代培养后获得nestin阳性细胞。

The nestin positive cells were harvested through primary culture and subculture .

新生鼠大脑皮质和中脑多巴胺神经元原代培养中NGF诱导c-junmRNA表达的研究

A study of c-jun mRNA expression of cortical and mesencephalic dopaminergic neurons induced by NGF from postnatal rats

5-HT受体亚型在原代培养大鼠脊髓背角神经元的表达

Expression of 5-HT receptor subtype mRNAs in primary cultured spinal dorsal horn neurons of the rat

结果：①原代培养获得了Nestin染色阳性的神经干细胞。

RESULTS ① Nestin positive neural stem cells were obtained through primary culture .

免疫组织化学方法证明，这些原代培养细胞呈nestin抗原阳性。

Nestin was positively expressing in these cells which were examined by immunohistochemistry .

结果说明，Leptin可促进体外原代培养的脂肪细胞甘油三酯的分解和脂肪代谢。

It is concluded that leptin can stimulate catabolism of triglyceride and mobilization of fat in adipose cell in vitro .

方法：原代培养牙髓成纤维细胞、牙周膜成纤维细胞，用MTT法测定细胞增殖，酶动力学方法测定ALP活性。

METHODS : Detection of the cell proliferation by MTT method and ALP expression by enzyme dynamics method .

体外灌注法分离大鼠肝脏Kupffer细胞及原代培养

Isolation of rat Kupffer cells by ex vivo perfusion and their primary culture

补肾法对去势兔动脉粥样硬化原代培养平滑肌细胞凋亡及血小板源生长因子amRNA等表达的影响

Effects of Kidney-Tonifying Method on Apoptosis and Expressions of PDGF-A mRNA and MCP-1 mRNA in Aorta Primary Cultured Smooth Muscular Cells of Atherosclerosis in the Ovariectomized Rabbit

方法：行PMC的原代培养及鉴定。

Methods : PMCs were primarily cultured and identified .

为研究胡黄连提取物对四氯化碳（CCl4）或半乳糖胺（DGaIN）损伤原代培养大鼠肝实质细胞的保护作用。

The protective effect of the extracts of Rhizoma Picrorhizae on primary cultured hepatocytes injured by CCl 4 and D-GaIN was studied .

体外原代培养胎鼠海马神经元，建立缺氧/复氧诱导损伤的海马神经元凋亡模型，观察EA对损伤海马神经元的保护作用。

Rat hippocampal neurons in primary culture were used , and an apoptosis model was induced by hypoxia / reoxygenation .

目的研究虎杖苷（PD）对CCl4损伤原代培养大鼠肝细胞的保护作用。

AIM To investigate the effects of polydatin ( PD ) on CCl 4 intoxicated primary cultured rat hepatocytes .

目的：利用原子力显微技术（AFM）观察原代培养的海马神经元超微结构及其相互间的连接结构。

Objective To investigate the ultrastructure and the connection structures between primarily cultured hippocampal neurons with atomic force microscopy AFM .

目的：原代培养小鼠成牙本质细胞，为诱导胚胎干细胞（ES细胞）向成牙本质细胞分化提供基础。

PURPOSE : To culture primary mouse odontoblast and to provide a base for study on inducing ES cells to odontoblast .