原位杂交组织化学

用原位杂交组织化学技术，对含Calbindin-D28KmRNA的神经元在大鼠脑干中的分布进行全了面观察。

Using in situ hybridization histochemistry , we examined the localization of the neurons containing calbindin-D_ ( 28K ) mRNA in the rat brainstem .

结果表明Zamboni氏固定液用于原位杂交组织化学与免疫组织化学结合法最佳，而4%多聚甲醛磷酸缓冲液用单纯原位杂交最佳。

The results showed that Zamboni 's fixative was the best for the combination of in situ hybridization histochemistry with immunohistochemistry while 4 % paraformaldehyde-phosphate buffer was best for in situ hybridization histochemistry .

臂旁核中的生长抑素mRNA阳性神经细胞&原位杂交组织化学研究

Somatostatin mRNA-containing neurons in the parabrachial nucleus of rats ── in situ hybridization histochemical study

电针可增强大鼠脑内前脑啡肽原mRNA的表达：原位杂交组织化学研究

Enhancement of preproenkephalin mRNA expression in rat brain by electroacupuncture : an in situ hybridization histochemistry study

GABAA和GABAC受体各亚单位基因在鲫鱼视网膜内的分布&原位杂交组织化学法研究

Localization of gaba_a and gaba_c receptor subunit mRNAs in carp retina ── an in situ hybridization analysis

大鼠实验性胃溃疡期间大脑皮层SS免疫组织化学和原位杂交组织化学研究

Study on the peptide and genetic expressions of somatostatin neurons in the rat cerebral cortex during experimental gastric ulcer

5-HT受体亚型mRNAs在大鼠背根神经节和三叉神经节的表达&原位杂交组织化学法研究

Expression of 5-HT receptor subtype mRNAs in the dorsal root ganglion and trigeminal ganglion of the RAT-IN situ hybridization histochemical study

方法地高辛标记的cRNA探针原位杂交组织化学和逆转录PCR技术。

Methods In situ hybridization histochemistry ( ISHH ) using digoxigenin labeled cRNA probes and reverse transcription polymerase chain reaction ( RT PCR ) .

选择性地对大鼠单根触须刺激后，通过原位杂交组织化学检测相应的躯体感觉皮质内即早基因c-fos的表达、局部定位以及与刺激强度的关系。

The expression of immediate early genes c fos , localization and relationship with the intensity of stimulation were studied in the somatosensory cortex with in situ hybridization after stimulating the vibrissa .

方法取10只健康雄性SD大鼠，体重200-250g，应用免疫组织化学法和原位杂交组织化学法检测正常大鼠脑内ADM及其mRNA的表达情况。

Methods 10 healthy male SD rats , weighing 200-250g , were used to measure the expression of ADM and ADM mRNA in the brain by immunohistochemistry and in situ hybridization .

原位杂交组织化学研究结果也显示大鼠腰段脊髓背根神经节中TRPV1mRNA与P2Y2mRNA共同表达。

Moreover , coexpression of TRPV_1 mRNA with P2Y_2 mRNA was determined in the rat lumbar DRG .

方法：用SABC免疫组织化学法和地高辛精标记的ET-1cRNA探针核酸原位杂交组织化学法，观察了兔输卵管组织ET-1的分布和ET-1mRNA的表达。

METHODS : The distribution of endothelin-1 and the expression of ET-1 mRNA in the rabbits uterine tubes were studied using SABC immunohistochemistry and in situ hybridization histochemistry with digoxigenin-labelled rat ET-1 cRNA probe .

氧惊厥时海马生长抑素神经元的原位杂交组织化学变化

Histochemical changes of somatostatin neurons in the hippocampus in hyperbaric oxygen-induced convulsion

方法：应用原位杂交组织化学法。

Methods : In this study , in situ hybridization histochemical method was used .

原位杂交组织化学技术。

In situ hybridization histochemical techniques .

原位杂交组织化学方法鉴定玉米根尖内源生长素对根冠特定基因正常表达的影响

Identification the Effect of Endogenous Auxin in Maize Root Tip on Its Root Cap Specific Gene Using in Situ Hybridization

借助原位杂交组织化学方法，定位、定量研究大鼠急性脊髓损伤后早期ET－1mRNA表达的时间和空间分布特征。

By means of in situ hybridization histochemistry ( ISHH ), the distributive particularities in time and location of the early expression of ET 1 messenger ribonucleic acid ( ET 1 mRNA ) in injured spinal cord tissues from rats were qualitatively and quantitatively measured .

方法采用核酸原位杂交免疫组织化学技术（ISHH）检测大鼠轻、中度脑损伤大脑皮层和海马BDNFmRNA表达的动态变化。

Methods Nucleic acid in situ hybridization histochemistry technique with an RNA probe and the computer image analysis were employed to detect the dynamic changes of BDNF mRNA in the cortex and the hippocampus after mild to medium brain injury .

为了解PcR－18基因在大鼠视网膜上的细胞定位，用原位分子杂交组织化学方法（ISHH），观察了代号PcR－18的视网膜特异基因表达细胞在大鼠视网膜内的分布。

The distribution of PcR 18 gene expressing cells in the rat retina was examined by means of in situ hybridization histochemistry method ( ISHH ) .

结果DNA斑点杂交、RNA斑点杂交、原位杂交和免疫组织化学染色均显示转染组有阳性信号；

Results the positive signals were observed in transfected keratinocytes by DNA dot blot , RNA dot blot , hybridization in situ and immunohistochemistry .

实验采用原位杂交，NADPH-NBT组织化学，RNA斑点印迹及蛋白质斑点印迹技术。

The experiment was performed by using in situ hybridization , NADPH NBT histochemistry , RNA dot blot and protein dot blot techniques .

方法采用原位杂交及免疫组织化学方法对16例正常早孕妇女蜕膜组织中的LIFmRNA及其蛋白的表达进行研究。

Method By immunohistochemical analysis and in situ hybridization , the LIF mRNA and protein expressions were observed in 16 cases of human decidua .

乳腺癌HER-2基因显色原位杂交与免疫组织化学检测对比研究

Comparison of chromogenic in situ hybridization and immunohistochemistry in determining HER-2 gene in status in breast cancer

所以，运用实时定量PCR、原位杂交和免疫组织化学方法检测了Frizzled3的mRNA和蛋白在动情周期的小鼠卵巢中的表达规律。

Here , we detected the expression and localization of Frizzled 3 mRNA and protein in the mice ovary during estrous cycle through real-time Q-PCR , in situ hybridization and immunohistochemistry .

大鼠纹状体边缘区内5-HT（2A）受体的分布&原位杂交和免疫组织化学研究

Distribution of 5 - ht_ ( 2a ) receptor in marginal division of rat striatuman ─ in situ hybridization and immunohistochemical study

作者用原位杂交和免疫组织化学PAP法双标记，在51例人肝硬变石蜡组织切片中检测HBVdna和HBsAg。

Hepatitis B virus DNA ( HBV DNA ) and HBsAg were detected in 51 human paraffin - embedded sections of liver cirrhosis by the double labelling technique of in situ hybridization and PAP method .

测定痛阈并分别以原位杂交和免疫组织化学技术探测颗粒曲管（GCT）细胞的EGFmRNA、EGF受体（EGFR）－免疫反应性（IR）及EGF－IR和垂体ACTH－IR。

The pain threshold was measured , and EGF mRNA , EGFR ( receptor ) - IR ( immunoreactivity ) and EGF-IR in granular convoluted duct ( GCT ) cells and the pituitary ACTH-IR were detected by in situ hybridization and immunohistochemistry techniques respectively .

取缺血心肌用原位杂交和免疫组织化学检测ICAM1mRNA及其蛋白表达水平，用酶法测定PMNs浸润数，TTC染色法测心肌梗死范围。

The levels of expression of ICAM 1 mRNA were evaluated by in situ hybridization and the protein by immunocytochemistry . The numbers of infiltrated PMNs were measured with MPO enzyme marker method and the myocardial infarction area was evaluated by TTC staining method .

方法原位杂交和免疫组织化学结合显微图像分析方法。

Methods In situ hybridization , immunohistochemistry and microscope image analysis were used .

显色原位杂交和免疫组织化学检测乳腺癌HER2/neu基因状况和蛋白表达的对照性研究

Comparision of HER2 / neu oncogene detected by chromogenic in-situ hybridization and immunohistochemistry in breast cancer