异源双链

异源双链DNA体外错配修复功能检测模型的构建及弥漫性大B细胞性淋巴瘤中错配修复系统的分析

Construction of Heteroduplex DNA and in Vitro Model of Functional Analysis of Mismatch Repair as Well as Its Application in the Diffuse Large B Cell Lymphoma

并将这种异源双链泳动分析法（heteroduplexmobilityasay，HMA）的结果与DNA序列测定结果比较。

The results from heteroduplex mobility assay ( HMA ) were compared with HIV 1 subtype results determined by DNA sequencing .

杂合子样品经过PCR变性、复性之后，其组分包含四种双链分子：两种同源双链、两种异源双链。

A heterozygous sample after denaturation , renaturation of PCR , contains four duplexes : two homoduplexes and two heteroduplexes .

HIV-1基因亚型分型：异源双链泳动分析法

Genetic Subtyping of HIV 1 Strains by Heteroduplex Mobility Assay

异源双链&SSCP法是简便实用的RB基因诊断方法。

Heteroduplex SSCP analysis is a simple and practical methods for gene diagnosis .

套式PCR扩增其包膜基因，用异源双链泳动分析法（HMA）分析基因亚型的变异。

The pro-DNA env gene of these HIV were amplified by nested PCR , then their subtype were analyzed by HMA .

λDNA与λplac5DNA的异源双链分子总长度与λDNA大致相同，单链环位于分子的41.5&47.2%之间。

The total length of the heteroduplex molecule between A DNA and λ plac 5 DNA was about the same as that of the A DNA molecule and a single stranded loop was located between 41.5-47.2 % of the molecule .

方法收集高度近视先证者178例，制备外周血白细胞基因组DNA，PCR扩增SHH基因3个外显子及其邻近内含子，SSCP异源双链法分析PCR产物，寻找可能的变异。

Methods Genomic DNA of 178 high myopic probands from peripheral leucocytes was extracted . All three exons of SHH were amplified by PCR , and possible variations in PCR products were detected using SSCP-heteroduplex analysis .

目的：评价异源双链分析（HET）和单链构象多态性分析（SSCP）在基因突变检测中的作用。

Objective : To assess the roles of single stranded conformation polymorphism ( SSCP ) and heteroduplex polymorphism ( HET ) in screening gene mutation .

方法采用改良的异源双链泳动法（HMA）与DNA序列分析法对14份HIV-1抗体阳性者样本进行亚型测定、分析和比较。

Methods By means of the modified heteroduplex mobility assay ( HMA ) and the routine DNA sequence analysis , the subtypes of 14 blood samples with HIV - 1 sera - positive were determined and analyzed and compared .

异源双链分析和单链构象多态性分析在基因突变检测中的作用

Comparison of heteroduplex analysis and SSCP in gene mutation screening

PCR/异源双链形成分析法在β地中海贫血产前诊断中的应用

The application of pcr / heteroduplex formation analysis to β thalassemia prenatal diagnosis

环式异源双链核酸分子单链核糖体失活蛋白的核糖核酸酶活性

RNase Activity of Single Chain Ribosome-Inactivating Proteins

环式异源双链核酸分子分隔式环形天线测向仪

Circular heteroduplex spaced-loop direction finder

多聚酶链反应结合酶切与异源双链形成分析检测白血病微小残留病

Detection of minimal residual disease in leukemia by polymerase chain reaction combined with restriction map and heteroduplex formation

异源双链泳动分析显示，病毒群复杂度在传代刚开始时低，以后一直较高。

HMA results indicated the complexity of virus groups was low at the very beginning of the passage and kept high later .

结论异源双链-SSCP法突变检出率高，经济、简便，值得在基因突变筛查中推广应用。

Conclusion Heteroduplex-SSCP analysis is simple , economical and more effective than SSCP or heteroduplex analysis alone and it may be widely applied in mutational screening .

另外，我们的结果还显示，在随后的cDNA克隆过程中，微生物的多种缺刻依赖型DNA修复系统对异源cDNA双链分子的修复也是上述这种人工嵌合序列的一个来源，但不是主要的来源。

In addition , our data also reveal that heteroduplex repairs by nick-dependent microbial DNA repair systems in the cDNA cloning step were found to produce chimeras , although it was not the major source under the present conditions .