异柠檬酸裂解酶

目的通过研究马尔尼菲青霉（PM）异柠檬酸裂解酶（ICL）基因转录水平与其酵母相生长的关系，初步探讨乙醛酸循环在PM致病中的意义。

Objective To study the relationship between transcriptional level of isocitrate lyase and development of Penicillium marneffei yeast and to investigate the significance of glyoxylate pathway in the pathogenesis of P.marneffei .

为了测定异柠檬酸裂解酶的活性，需要实时测定NADH量的减少。异柠檬酸在异柠檬酸裂解酶催化下形成乙醛酸，乙醛酸可通过乳酸脱氢酶和NADH还原成甘醇酸酯。

To determine the activity of the isocitrate lyase , the glyoxylate formed during catalysis was reduced to glycolate by lactate dehydrogenase with the concomitant oxidation of NADH .

结核杆菌H（37）Rv异柠檬酸裂解酶基因的克隆及表达

Clone and Expression of Isocitrate Lyase Gene in Mycobacterium tuberculosis H_ ( 37 ) Rv

纯化的目的蛋白异柠檬酸裂解酶的酶活性为16.7u/mg。

The enzyme activity of the purified protein was 16.7u/mg .

结果表明，NCS明显抑制光下培养的离体萝卜子叶叶绿素含量及鲜重增加，对异柠檬酸裂解酶及羟基丙酮酸还原酶活性也显示出明显的抑制作用。

The results indicated that NCS markedly inhibited the increase of chlorophyll content and the fresh weight of excised radish cotyledons during a period of growth in light . The isocitrate lyase and hydroxypyruvate reductase activities were also significantly inhibited by NCS .

马尔尼菲青霉异柠檬酸裂解酶的表达与其酵母相生长的关系

The relationship between expression of isocitrate lyase and development of penicillium marneffei yeast

异柠檬酸裂解酶&抗结核药物的新靶点

Isocitrate Lyase-a new target in TB drug research

种子中异柠檬酸裂解酶活性的测定

Determination of Isocitrate lyase Activity in Seeds

赤霉酸对小麦和大麦糊粉层细胞中异柠檬酸裂解酶形成的作用

The effect of gibberellic acid on the formation of isocitrate lyase in the aleurone cells of wheat and barley

伴随着种子的发育进程，6－磷酸葡萄糖脱氢酶、异柠檬酸裂解酶、异柠檬酸脱氢酶和琥珀酸脱氢酶的活性均有不同程度的增强。

There were gradually increased in activities of glucose-6-phosphate dehydrogenase , isocitrate lyase , isocitrate dehydrogenase and succinate dehydrogenase .

结核分枝杆菌异柠檬酸裂解酶基因的克隆表达及酶学性质研究

Molecular cloning , expression , purification and properties of isocitrate lyase gene from Mycobacterium tuberculosis H_ ( 37 ) Rv

其中，酶学水平上，比较了两菌株在初级代谢过程中的四种关键酶酶活，分别是葡萄糖-6-磷酸脱氢酶、柠檬酸合成酶、异柠檬酸裂解酶和苹果酸合成酶。

Glucose-6-phosphate dehydrogenase , citrate synthase , isocitrate lyase and malate synthetase are the four key enzymes involved in the primary metabolism .

异柠檬酸裂解酶、α-和β-淀粉酶的活性先上升，然后下降；

In the meantime , the activities of α - and β - amylase and isocitrate lyase in seeds rose initially , and then decreased .

乙醛酸循环关键酶，异柠檬酸裂解酶和苹果酸合成酶分别为正常尿素培养物的2.6和2.8倍。

The activities of the key enzymes of the glyoxylic acid pathway , isocitrate lyase and malate synthetase are 2.6 and 2.8 times of those the control culture .

在油分旺盛合成期，6－磷酸葡萄糖脱氢酶和异柠檬酸裂解酶的活性均达到了最大值，而此时，异柠檬酸脱氢酶和琥珀酸脱氢酶的活性均增加较慢；

The activities of glucose-6-phosphate dehydrogenase and isocitrate lyase increased to maximum , however , the activities of isocitrate dehydrogenase and succinate dehydrogenase increased slowly when oil synthesized rapidly .