豆甾醇

结果：从青葙子乙醇提取物的石油醚部位分离得到化合物1～3，分别鉴定为β-谷甾醇（β-sitosterol）、棕榈酸（Palmiticacid）、豆甾醇（stigmasterol）；

Results : From the petrol-ether fraction of the EtOH extract of Semen Celosiae , 3 compounds were isolated and identified as β - si-tosterol ( 1 ), palmitic acid ( 2 ) and stigmasterol ( 3 ) .

混合植物甾醇中豆甾醇和β-谷甾醇的高效液相色谱分析

HPLC Analysis of Stigmasterol and β - Sitosterol in Phytosterols

实验结果表明，以环己酮为溶剂，混合植物甾醇经过3次分级结晶后，b谷甾醇含量达到87%；以环己酮或正戊醇为溶剂，通过5次重结晶后，豆甾醇含量达到92%。

The experimental results show that the content of b-sitosterol is more than 87 % after the third fractional crystallization in cyclohexanone and the content of stigmasterol is more than 92 % after the fifth crystallization in n-pentanol or cyclohexanone .

本方法相对标准差为1.1%～6.9%；菜油甾醇、豆甾醇、β-谷甾醇的回收率的RSD值范围为3.0%～4.7%。

The results showed that the RSD of this method was ranged from 1.1 % to 6.9 % and the RSD of recovery ranged from 3.0 % to 4.7 % .

实验结果表明，混合植物甾醇及其乙酸酯的响应时间在25min以内，且各种甾醇及其酯的分离效果较好，采用峰面积归一法能够快速准确地测定混合甾醇中豆甾醇乙酸酯和β谷甾醇乙酸酯的含量。

The experimental results showed that phytosterol acetates could be separated within 25 minutes and determined accurately by the peak-area normalization method .

豆甾醇提取纯化工艺研究

A Study on Technology of Extracting and Purification of Stigmasterol

植物油中主要含有谷甾醇、豆甾醇、菜油甾醇等多种植物甾醇。大豆油多元醇的制备和应用研究进展

Progress of Preparation and Application of Soybean Oil-based Polyol

结晶法分离精制混合植物甾醇中β-谷甾醇和豆甾醇

Separation and Purification of Stigmasterol and β - Sitosterol from Phytosterol by the Crystallization Method

应用高效液相色谱对豆甾醇乙酸酯、β谷甾醇乙酸酯和混合甾醇乙酸酯进行了分析测定；

Stigmasterol acetate , β - sitosterol acetate and mixed phytosterol acetates were analyzed by HPLC .

结果表明，两种组分均主要含有豆甾醇和β-谷甾醇以及少量菜油甾醇物质。

The results showed that they both are containing stigmasterol ,β - sitosterol , and small quantities of campesterol .

实验结果表明：豆甾醇与琥珀酸酐的反应产物为豆甾醇琥珀酸单酯；磺基琥珀酸单酯盐的研究及应用

The experimental results showed that the product of reaction was stigmasteryl hemisuccinate . The Research and Application of Di-sodium Sulfo-succinate Mono Est-er

其中豆甾醇、没食子酸乙酯、苯甲酸为首次从该植物中分离得到。

And the Compounds of stigmasterol , ethyl gallate and butanedioic acid were obtained from this plant first time among these componds .

现代药理研究证明，它富含大黄素等蒽醌类衍生物、豆甾醇类、脂肪酸、氨基酸以及多种微量元素，更是丰富了其应用范围。

Modern pharmacological studies have shown that it is rich in anthraquinones materials and more other composition which extend the application range .

确定了9种化合物的结构，其中豆甾醇、β-谷甾醇、壬二酸等为首次在本属真菌中分离得到。

Nine compounds structure were identified , stigmasterol ,β - sterol , azelaic acid were also obtained from this genus the first time .

精制甾醇中大豆总甾醇含量为85%，其中β-谷甾醇42.3%、豆甾醇18.1%、菜籽甾醇24.5%。

The phytosterol content in the purified sample was 85 % , including 42.3 % Betasitosterol 、 18.1 % stigmasterol 、 24.5 % campesterol .

用色谱、光谱以及与标准品对照等方法鉴定代谢产物的结构分别为菜子甾醇和豆甾醇。

The products were identified to be brassicasterol and stigmasterol respectively based on their chromatographic and spectral properties as well as by comparison with the authentic samples .

结果：各包装饮片的豆甾醇的含量基本无变化，聚乙烯塑袋包装的饮片微生物和水分均远高于真空复合包装和纸塑包装。

Results : The contents of stigmasterol were much the same in three kinds of package , but the contents of microbe and water in polyethylene package increased more rapidly than those in vacuum package and paper-plastic package .

结果表明：在实验条件下甾醇酯化反应完全，β-谷甾醇乙酯和豆甾醇乙酯的红外特征吸收峰面积比与两者的含量比成线性关系。

The results show that the esterification is carried out completely under the experimental condition and the relationship between the ratio of the feature peak area of IR spectra and the ratio of the contents of stigmasterol acetate and β sitosterol acetate is linear .

从油脂精炼副产物中提取的植物甾醇主要包括β-谷甾醇、豆甾醇、菜油甾醇和菜籽甾醇，能抑制人体对胆固醇的吸收，是一种降低血清胆固醇的功能活性成分。

Phytosterols , including J3-sitosterol , stigmasterol , campesterol and brassicasterol mainly , were obtained commonly from by-product of refining vegetable oil . Phytosterols have been use as a novel functional food ingredient with plasma cholesterol lowering activity which works by inhibiting the absorption of cholesterol .