终止序列

作为应用程序终止序列的一部分（或需要时），它可以

As part of the application termination sequence ( or when required ), it

实验中还对目的基因之后的Nos终止序列区进行了扩增，通过NosTer。

Nos terminating sequence which was behind thymosin gene was amplified .

具有代表性的LMP1基因羧基末端PCR产物采用四色荧光终止序列技术进行DNA序列分析。

DNA sequencing of the representative PCR products of carboxyl-terminus of LMP1 gene was analyzed by using four-colored fluorescence terminator sequencing technique .

还探索了转录终止序列对融合基因蛋白表达水平的影响，构建了高效表达融合蛋白的载体－宿主系统。

Based on the results , a vector-host system was established for the high expression of fusion proteins .

用户可以用该按钮终止音频序列。

With this button the user can abort the audio sequence .

在数据传输中，被起始和终止标志序列括起来并包括标志序列在内的连续二进制位的序列。

In data transmission , the sequence of contiguous bits bracketed by and including beginning and ending flag sequences .

所有基因均没有内含子，尤其是在CDS的起始区和终止区，序列完全相同。

All the genes are without introns , and totally identical in the CDs region .

阳性重组质粒经PCR、酶切鉴定，用双脱氧链末端终止法进行序列测定。

The positive recombinant plasmid was identified by PCR , enzyme digestion . The nucleotide sequence of CSP3 ′ ending gene was determined by the dideoxy chain termination method .

作者作出此修改是为了确保对于终止的RM序列，在RM的目的地端能够进行有效的资源回收。

The authors made this change to ensure that the protocol enabled efficient resource reclamation on the part of the RM Destination for terminated RM Sequences .

方法应用逆转录巢式PCR技术，从38例III/2a型HCV感染患者血清中，扩增HCV部分包膜区基因片断（nt1460～1582，HCVJ6），纯化后直接采用双脱氧链末端终止法进行序列分析。

Methods The RNA ( nt1460 1582 , HCV J6 ) extracted from plasma of 38 patients infected with genotype III / 2a HCV were transcribed , amplified , purified and directly sequenced by using RT nested polymerase chain reaction ( PCR ) and dideoxynucleotide chain termination method , respectively .

PCR产物纯化后直接经双脱氧链末端终止法测定核苷酸序列。

The amplified product with 238 bp was purified and then directly sequenced by dideoxy nucleotide chain termination method .

方法病毒粒RNA经逆转录合成cDNA，经聚合酶链反应（PCR）扩增，产物纯化，采用双脱氧链末端终止法进行核苷酸序列测定。

Methods Virion RNA was transcribed into cDNA by reverse transcriptase , cDNA amplified by PCR , the productions of PCR were purified .

E.coli和Yeast基因起始与终止密码子邻近序列碱基保守性、关联性的对比研究

The Contrast Study of Base ′ s Conservative Character and Correlative Character between E.coli and Yeast Gene for Adjacent Sequence of Initiation Codon or Termination Codon

方法：采用PCR和OverlapPCR方法构建抗CD3/抗CD20微型双功能抗体，并用双脱氧终止法测定DNA序列；

Methods : PCR and overlap PCR were used to construct anti CD3 / anti CD20 Diabody . DNA sequence was analyzed by the Terminus of Dideoxy Nucleotide .

发现酵母基因编码区和已知DRF的起始密码子总是位于长距离不出现终止密码的位置序列上游紧邻最后一个终止密码子的ATG；

This result shows that the starting codon of yeast gene is always the ATG which is in close neighbor to the last terminal codon upstream .

真核基因起始与终止密码子旁侧序列的特征对于确定cDNA开放阅读框架（ORF）和预测基因组序列中的编码区（CDS）非常重要。

The characters of sequence flanking the start codon and stop codon in eukaryotic genes play an important role in defining the open reading frame ( ORF ) in cDNA sequences and coding region ( CDS ) in genomic DNA sequences .

采用反转录-PCR方法，分节段扩增汉坦病毒A9株M基因片段cDNA，并克隆入PGEMT载体中，用双脱氧末端终止法直接测定序列。

The M RNA segment of Hantaan virus Chinese isolate A9 was reverse-transcribed into the first strand of cDNA and amplified into cDNA fragments which were cloned into pGEM-T vector . The positive clones were sequenced on double strand level by dideoxy chain termination method .

因为RM目的地端分配序列号标志符，一旦序列被终止，RM的目的地端能够清除所有与终止的序列相关的资源。

Because the RM Destination assigns the Sequence identifier , once the Sequence has been terminated , the RM Destination can purge all state associated with the terminated Sequence .