引物酶

DNA引物酶抑制剂筛选方法的建立及应用

The establishment of a screening method of DNA primase inhibitors

DNA引物酶的提取分离与引物合成活性的研究

Extraction and primer synthesis activity of DNA primase

地高辛标记引物酶显色法检测HBV基因多态性及应用

Application of enzyme coloration with digoxin-labeled primers for detection of HBV gene polymorphism

茶多酚对小鼠肿瘤L2细胞DNA引物酶-多聚酶α复合体活性的抑制作用

Inhibition effect of tea polyphenol on the activity of DNA primase-polymerase α complex from tumor L_2 cells in mice

用地高辛标记引物酶显色法，检测了63例e抗原阴性慢性肝炎HBV基因多态性。

Detection of HBV gene polymorphism was carried out by using of enzyme coloration with digoxin-labeled primers in 63 cases of eAg ( - ) chronic HBV infections .

用磷酸纤维素、DEAE纤维素、单链DNA纤维素和AMP-Sepharcse的顺序柱层析法从人慢性骨髓细胞性白血病K562细胞中纯化了DNA聚酶α与DNA引物酶的复合物。

DNA polymerase α - DNA primase complex from K562 cells , a human chronic myelogenous leukemia cell strain , was purified by sequential chromatography of phosphocellulose , DEAE-cellulose , single-strand DNA cellulose and AMP-sepharose columns .

单纯疱疹病毒1型解螺旋酶-引物酶编码基因重组杆状病毒载体的构建与鉴定

Construction and identification of recombinant baculovirus vectors of genes encoding helicase-primase of HSV-1

规范了DNA、引物、Taq酶等试剂质量，以保证数据的稳定性和可重复性；

In order to guarantee the stability and repeatability of data , standardize the reagent quality of DNA , primer , Taq et al .

合成两条长链部分互补引物，经Klenow酶延伸补平是获得目的基因的重要方法之一。

Klenow polymerase mediated extension of two long complementary oligonucleotides is one of the methods .

用大肠杆菌大片段DNA多聚酶Ⅰ延长引物法检测DNA引物酶比活性为8753U／mg，酶总获得为22.1%。

DNA primase activity was assayed with E coli . DNA polymerase ⅰ Klenow fragment extended primers . The specific activity of the enzyme was 8 753 U / mg protein .

方法：以特定引物和16mer的随机引物作为延伸引物，利用Taq酶标记DNA探针。

Methods : Based on PCR principle , DNA was labeled using specific and random primers . The E.