引物合成

引物合成和DNA序列测定由Takara公司完成。

Primer synthesis and DNA sequencing were completed by Takara .

用随机寡核甘酸引物合成法（ROPS）测定DNA链断裂程度；

Random oligonucleotide primed synthesis was used to measure the extent of DNA strand breaks .

利用PCR反应得到了26个水稻蛋白激酶的编码序列；为了提高同源重组的效率，降低引物合成的成本，试验采用两次PCR方法使重组位点达到50bp，明显提高了转化效率；

And 26 encoding sequences of these kinases were obtained through two rounds of PCR , enhancing the efficiency of transformation ;

方法从日本血吸虫成虫中提取总RNA，用Oligo（dT）12-18为引物合成cDNA，并用20个引物PCR法扩增其中的cDNA。

Method Total RNA from adult S. Japonicum worm pairs was isolated . The cDNA were synthesized with Oligo ( dT ) 12-18 when twenty of primers were used for PCR , the specific cDNA fragments were amplified .

分子量标准引物定位合成（MarkerPrimer-directedSynthesis，简称MPDS）乃一种DNA碱基对梯度片段的简易合成术。

Marker primer-directed synthesis ( MPDS ) is a technique that allows the easy synthesis of DNA base pair ( bp ) ladders .

方法：提取新生大鼠肝脏总RNA为模板，以寡聚dT为引物逆转录合成第一链cDNA，再以我们设计的PCR引物进行PCR扩增获取大鼠肝再生增强因子编码区双链cDNA；

Methods : Total RNA of new born rat liver was extracted as a template , first strand of cDNA was synthesized by reverse transcription with olig ( dT ) - primer and then amplified by PCR with the primers designed by ourselves .

荧光定量PCR方法采用双标准曲线法对CIRPmRNA进行相对定量检测，先从GenBank上公布大鼠的CIRP和持家基因GAPDH的核酸序列设计两对特异性引物并合成。

CIRP mRNA relative expression quantity was detected by the double standard curve in the real time PCR method . First , two pairs of specific primers were designed on the nucleic acid sequences of Housekeeping gene GAPDH and rats CIRP , which have been published in the GenBank .

开发一套辣椒与茄子都通用的分子标记，不仅可以节约引物的合成成本，用于不同物种间EST-SSR的比较研究，更能了解基因在物种间的进化，从而加速对基因组结构和功能的了解。

Developing a suite of molecular marker between pepper and eggplant was testified feasible , which will not only save the primer cost , make the species comparative research , but also realize the evolution of genes in different species , and consequently find out the genome structure and function .

一种分子量标准的引物定位合成

Primer-directed Synthesis of a Molecular Weight Marker

本文从该杂交瘤细胞株提取细胞总RNA，以oligo-d（T）为引物反转录合成cDNA第一链。

Total RNA was extracted from the cells and was reverse transcribed to the first strand cDNA using oligo-d ( T ) as a primer .

在黄鳝二价染色体上，运用地高辛配基标记的随机引物原位DNA合成技术诱导出了明暗相间、基本稳定且类似于R带的带状结构。本文对该结果进行了较详细的分析和讨论。

The dark bands alternating with light bands , relatively stable and R-band-like structure was showed on the pachytene bivalents of Rice-fleld eel ( Monoplerus albus Zuiew ) by using the random-primed in situ digoxigenin-labelled DNA synthesis technique , and the results were analysed and discussed in detail .

提取青岛文昌鱼18小时神经胚中期mRNA，以5′脱磷的NotⅠoligo（dT）18为引物，反转录合成cDNA。

MRNA isolated from Qingdao amphioxus neurulae of 18 hour period was reverse transcripted into cDNA using Not ⅰ - oligo ( dT ) 18 as a primer .

通过此方法纯化的RNA可用于各种标准的下游技术，如RT-PCR、polyA+RNA选择、差异显示技术、Northern点杂交和狭线杂交、引物延伸、cDNA合成、陈列表达分析和表达芯片分析等。

The purified RNA is ready for use in standard and downstream applications such as RT PCR , polyA + RNA selection , differential display , Northern dot and slot blotting , primer extension , cDNA synthesis , expression array and expression chip analysis .

方法：特定寡核苷酸引物的设计、合成与纯化；

METHODS : The design , synthesis and purification of oligonucleotide primers ;

DNA引物酶的提取分离与引物合成活性的研究

Extraction and primer synthesis activity of DNA primase

结果与结论：Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果，且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。

Results and conclusion : Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system .