引物二聚体

减少非特异性扩增产物及引物二聚体的生成，提高检测的专一性。

It enhances the specificity of detection due to decreasing the products from non-Specific amplification and primer dimerization .

经熔解曲线分析，其扩增产物中未检出非特异性产物和引物二聚体；

A melting curve analysis shows that , the quantity of unspecific PCR products have witch fewer primer dimers than other amplification products .

如图6和7所示分别为Ⅰ、Ⅲ胶原的扩增、溶解曲线和溶解峰，说明Ⅰ、Ⅲ胶原是特异性基因表达，无引物二聚体及非特异性扩增。

As shown in Figure 6 and 7 , respectively ⅰ,ⅲ collagen in the amplification curve and dissolution peaks , indicating ⅰ,ⅲ collagen is a specific gene expression , no primer-dimers and nonspecific amplification .

结论1.所设计的通用引物能够一次对所研究的七种病原菌进行扩增，且条带结果清晰，未见明显的非特异性条带和引物二聚体。

Universal primers could successfully amplify seven kinds of pathogens without obvious non-specific amplification or dimers . 2 .