方法采用经典的基因敲除法选择性缺失小鼠PASG基因第10-12外显子，并以组织学及westernblot法检测基因敲除鼠的中枢神经细胞形态和基因改变。
10-12 exons of murine PASG gene were selectively deleted with classical knockout method . Histological analysis and western blot assay were employed to examine CNS cell morphology and gene expression in mutant mice .
基因敲除法