体细胞核移植技术

体细胞核移植技术为生产转基因动物核心技术之一，该技术的优化和成熟，对于成功生产转基因动物至关重要。

Somatic cell nuclear transfer ( SCNT ) is a key technique for producing transgenic mammalian , so optimization of this technique is important for improving the total efficiency of transgenic animal production .

体细胞核移植技术在制备转基因动物方面有许多优点，例如：可以快速扩大转基因动物的群体，且能保持外源基因稳定传递。

The technique of somatic cell nuclear transfer ( SCNT ) has many advantages when used in transgenic animal production , such as increasing the number of transgenic animal rapidly without losing foreign gene .

激活是体细胞核移植技术的关键步骤。

Activation is a key step of somatic cell cloning .

动物体细胞核移植技术的研究现状

Current Status of Nuclear Transplantation of Animal Somatic Cells

牛去透明带卵体细胞核移植技术的应用研究

Application of the zona-free technique for production of bovine somatic nuclear transfer embryos

体细胞核移植技术和诱导多功能干细胞技术的研究比较

Comparison of Somatic Cell Nuclear Transfer Technique and Induced Pluripotency Stem Cells Technique

牛体细胞核移植技术

Investigation of Bovine Somatic Cell Nuclear Transfer

哺乳动物体细胞核移植技术研究进展

Progress of Somatic Nuclear Transfer in Mammal

不借助于显微操作仪的小鼠体细胞核移植技术研究

Mouse Somatic Nuclear Transfer Without Micromanipulators

自从多莉诞生以来，体细胞核移植技术已经有了长足的发展。

Since Dolly , the use of nuclear transfer of an adult genome has come a long way .

结果说明，本研究的牛去透明带双半卵体细胞核移植技术已可达到克隆胚胎批量生产应用的水平。

In conclusion , the bovine somatic nuclear transfer technique with zona-free 2-half cytoplast manipulation can result in a satisfactory batch cloned embryo production .

本文综述了体细胞核移植技术的研究进展、技术方法，以及它在医学领域巨大的应用价值和前景。

This text overviews the somatic cell nuclear transfer technique research progress , technique method , and its important application value and foreground in medical science .

自多莉诞生以来，体细胞核移植技术在家畜中的应用的到了长远的发展。

Since the " Dolly " birth , the application of somatic cell nuclear transfer technology in livestock is ascending in the field of animal husbandry .

用体细胞核移植技术生产转基因动物是目前一种最新、最有效的生产转基因动物的方法，而转基因体细胞系的建立是用这一方法生产转基因动物的基础。

Somatic cells nuclear transfer offers an efficient and practical method of producing transgenic animals , and the establishment of transgenic somatic cell lines are the basic of this method .

体细胞核移植技术在多年的研究发展中日益完善，但核移植效率仍然较低，限制了体细胞核移植技术的应用。

Somatic cell nuclear transfer ( SCNT ) technology has been improved steadily in recent years , but the efficiency of nuclear transfer is still low , which limits the application of SCNT technology .

体细胞核移植技术过程复杂，步骤较多，本研究分析了卵巢保存时间、去核方法和胚胎培养液三个因素对生产克隆胚胎效率的影响，以期获得优化的核移植方法。

Somatic cell cloning is a complex and easier technology . In this research , we studied effects of ovary storage , enucleation methods and embryo culture medium on the preimplantation development of clone bovine embryos , to optimize the procedures of nuclear transfer .

猪体细胞核移植及相关技术研究

Research of Porcine Somatic Cell Nuclear Transfer and Related Technology

1997年首例体细胞核移植绵羊在世界上诞生，之后不久出现了体细胞核移植小鼠及牛，并通过体细胞核移植技术制作了转基因动物。

The first sheep cloned from cultured mammary epithelia cell was born in 1997 , soon after that the cloned mice and calves were reported , and nuclear transfer of somatic cells has been used successfully to make transgenic animals .