基因打靶技术

基因打靶技术是一项新兴的分子生物学技术，是利用外源DNA与受体细胞染色体DNA上的同源序列之间发生重组，并整合在预定位点上，从而改变细胞遗传特性的方法。

Gene targeting is a rising technology in molecular biology , which is defined as the introduction of exogeneous DNA to specific site in genome by homologous recombination , and consequently change the hereditary character of the cell .

基因打靶技术在小鼠ES细胞上被广泛应用，但由于大家畜很难制备向生殖系分化的ES细胞系，所以基因打靶的应用变得非常困难。

Gene targeting technology has been widely applied to mouse ES cell , but more difficult to livestock species because ES cell of livestock species could not differentiate into germ cell line .

同时采用基因打靶技术对水稻Bel基因进行定点敲除，能有效地获得苯达松敏感致死株。

Further more , knock out the gene Bel using the gene targeting in rice can obtain the bentazon susceptive rice .

目的：利用体细胞基因打靶技术来获得定点整合有重组人瑞替普酶（rPA）的山羊胎儿成纤维细胞株。

AIM : To obtain a goat fetal fibroblast cell line with site-directed recombination of reteplase ( rPA ) minigene by somatic cell gene targeting technique .

用基因打靶技术构建恶性疟原虫MSP1转基因鼠疟模型及其应用

Construction of Transgenic Mouse Malaria Parasite Carrying MSP 1 of Plasmodium Falciparum by Gene Targeting Technique and Its Application

在过去的20年里，出现了一些新的转基因方法，包括精子介导、反转录病毒介导、携带外源基因体细胞的核移植、ES细胞基因打靶技术等。

In the past 20 years , some new approaches have been used . These include sperm mediated DNA transfer , retroviral mediated DNA transfer into oocytes , somatic cell carrying exogenous genes nuclear transfer , the use of embryonic stem ( ES ) cell .

本实验成功应用原代成纤维细胞基因打靶技术，首次获得了在β-酪蛋白基因座定点整合IL-11的GEF细胞株，为进一步核移植做好了准备。

The gene-targeting technique in primary fibroblast was successfully used in GEFs in this experiment , and the cell lines were finally obtained that the human IL-11 gene was integrated in 3 - casein gene locus of goat .

基因打靶技术通过利用同源重组可以在一定程度上实现外源基因的定向整合；

Gene targeting by homologous recombination can realized targeted integration .

基因打靶技术在乳腺生物反应器中的应用

The Application of Gene Targeting in the Mammary Gland Bioreactor

利用基因打靶技术研制人类疾病小鼠模型

Study of Human Disease Mouse Model by Gene Target

动物基因打靶技术的原理与应用

The Principle of Gene Targeting and its Application

基因打靶技术的原理及操作

The principle and experimental practice of gene knockout

基因打靶技术的应用研究

Studies on the Application of Gene Targeting

基因打靶技术及其影响因素

Gene Targeting and Its Affecting Factors

以重复序列为靶位点的多位点基因打靶技术，将部分解决目前存在的打靶效率低、安全性差等问题。

The technique of multiple locus gene targeting on repetitive sequences will resolve a part of these problems .

基因打靶技术的应用前景广阔，它的产生是遗传工程领域的一次革命。

The future of gene targeting 's application is bright and its appearance is a revolution in genetic engineering .

本文就基因打靶技术及其在人类疾病动物模型研究中的应用做一介绍。

Gene targeting technology and its application in research of animal model of human disease were briefed in this paper .

目的利用基因打靶技术构建变异链球菌葡聚糖结合蛋白D基因（gbpD）失活株，用于葡聚糖结合蛋白D基因功能的研究。

Objective To construct an insertional inactivation mutant of Streptococcus mutans glucan-binding protein D for the further functional study of the protein .

而利用基因打靶技术研究蛋白分泌途径的重要基因的功能有利于了解纤维素酶分泌输出的机制。

And , application of gene targeting to study the important genes involved in the protein secretion will contribute to understanding of the mechanism of cellulase transportation .

基因打靶技术的核心是同源重组，由于结果的可预见性和准确性，基因打靶已成为功能基因组学研究的常规技术。

With its accurate and predictable result , gene targeting became one of the normal technologies being used in genomics study . And the core technology of gene targeting is the homologous recombination .

但基因打靶技术目前主要应用于细菌，酵母和一些丝状真菌，在高等植物中应用极少。

But gene targeting was mainly applied to such field as bacterium , yeast and some epiphyte at present , seldom used in higher plants because of the lower frequency of homologous recombination .

基因打靶技术是建立在胚胎干细胞和同源重组技术之上，可对基因组进行定位修饰的实验方法，尤其可以在转基因动物的遗传性状修饰中起到巨大的作用。

Established upon the embryo stem cell technique and homologous recombination , gene targeting has been widely used in the genome specific manipulation , especially applied in the genetic trait modification of transgenic animal .

利用动物转基因技术和动物克隆技术，再结合基因打靶技术，人们开展了基因的定点修复、遗传缺陷的治疗、动物不利基因的敲除失活等一系列的动物基因组修饰的理论和应用研究。

With the combination of gene targeting , animal transgenic and animal cloning technologies , there have been a series of theoretical and applied research of animal genomes modified , such as fixed-point gene repair , the treatment of genetic defects and inactivation of animal unfavorable gene .

所幸我国鱼类核移植技术较为成熟，结合细胞基因转移、基因打靶和核移植技术，有望在不久将来获得稳定整合的纯化转基因鱼。

In combination of techniques of gene transfer in cultured fish cells , including embryonic or somatic cells , and nuclear transplantation , stable transgenic fish line with important economic traits will be generated in a near future .

基因敲除、精细突变和条件性基因打靶技术建立的基因打靶突变小鼠在人类遗传病机理研究、基因治疗和基因功能研究方面都有着重要作用。

Gene targeted mouse mutants , which have been generated by the techniques of the gene knockout , the subtle gene targeting and the conditional gene targeting , will play the important role in the mechanisms of pathogenesis of human genetic diseases , gene therapy and gene function researches .

随机插入的转基因方法严重阻碍了外源基因在转基因动物体内的有效整合和稳定遗传及表达，基因打靶技术可以解决这个问题。

Transgenic techniques with random integration have extremely decreased the efficiency of integration , hereditation and expression of external genes in transgenic animals .

目的：将小鼠胚胎干细胞中凝血因子Ⅸ（mFⅨ）基因定向敲除，为建立血友病B的转基因小鼠模型奠定基础；摸索建立ES细胞基因打靶技术体系。

Objective : To knockout the mouse coagulation factor ⅸ( mF ⅸ) gene in embryonic stem ( ES ) cells , and establish the technique system of ES cell gene targeting .