抗除草剂基因

用基因枪法将抗除草剂基因导入小麦栽培品种的研究

Studies on the Transfer of Herbicide-resistance Gene into Cultivated Wheat by Biolistics

抗除草剂基因（BAR）转基因水稻的毒性试验

Toxicity of anti - herbicide gene ( BAR ) transgenic rice

用基因枪法成功地把抗除草剂基因bar导入了4个优良旱稻品种，获得了大量转基因抗性植株。

The gene bar was transferred into 4 elite upland rice cultivars using particle bombardment and lots of plantlets with herbicide tolerance were obtained .

利用花粉管通道法，用含抗除草剂基因bar及抗虫基因pin2的质粒pTW-α转化中籼HD357。

Via pollen - tube pathway the indica rice HD - 357 was transformed with plasmid pTW - α containing Bar gene and PIN ⅱ gene .

抗除草剂基因导入旱稻（Oryzasativa）栽培品种

To Transfer Anti - herbicide Gene into Upland Rice ( Oryza sativa ) Cultivars

农作物抗除草剂基因工程研究进展

Achievements in Herbicide Resistant Gene Engineering of Crops

农杆菌介导的抗除草剂基因转入两系杂交稻恢复系的研究

Agrobacterium mediated transformation of the restorer lines of two line hybrid rice with bar gene

不同预处理对小麦幼胚抗除草剂基因转化效率的影响

Effect of different pre-treatment on the frequency of transformation of herbicide-resistant gene of wheat embryo

目的研究细胞质抗除草剂基因对谷子性状和发育的影响。

【 Objective 】 The effect of cytoplasmic herbicide resistant gene in millet plants was studied .

结论细胞质抗除草剂基因对谷子的生长和性状发育有影响，但利用杂交种可避免细胞质抗性产生的不利影响，获得丰产性；

【 Conclusion 】 The plant characters and development of foxtail millet were negatively affected by the cytoplasmic herbicide resistant gene .

种植于北达科他州的转基因油菜中植入了抗除草剂基因，使之对农达等除草剂产生抗性。

Transgenic canola plants in North Dakota had received genes making them resistant to herbicides , such as the weed killer Roundup .

结果表明，外源基因转化球孢白僵菌原生质体时，可以以抗除草剂基因为转化筛选标记。

The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana .

目前，抗除草剂基因、抗虫基因以及和木材质量相关的基因已被分离并应用于林木遗传工程。

Genes involved in important agricultural traits such as herbicide resistance , insect resistance , and wood quality have been isolated and have been used to genetically engineer trees .

目前广泛采用的抗菌素或抗除草剂基因作为植物转化筛选标记基因可能带来转基因逃逸，因此寻找能够用于植物转化的来源于植物本身的筛选基因是解决这一问题的方法之一。

Because of the concern about escape of antibiotic or herbicide resistant transgenes from transgenic crops , selectable marker genes from plant origin would be an alternative choice for plant transformation .

利用除草剂草甘膦处理含4个基因的转基因植株，它们表现出对草甘膦具有不同的抗性，推测是由于抗除草剂基因在不同个体中表达强弱不同所致。

These transgenic plants with four genes were treated by herbicide glyphosate and performed different resistance to glyphosate , which would be resulted from the expression of herbicide-resistant gene in different degrees between individuals .

在综述了抗除草剂基因的转化方法及其抗性机理的同时，还讨论了其安全性问题，并对今后抗除草剂转基因作物的研究与应用进行了展望。

In the paper we reviewed the method of ge-netic transformation and the mechanism of herbicide resistant genes . We also discussed the biosafety and prospected the study and application of herbicide resistant transgenic crops .

目前植物遗传转化中多以抗生素抗性基因和抗除草剂基因为标记基因，对生态环境和人类健康具有潜在的威胁，从而限制了转基因植物在商业生产中的推广利用。

However , it is also a potential hazard to the environment and human health when making use of bio antibiotic or herbicide resistant genes as the selectable marker genes during transformation , which limits the prospects of the application for commercial production .

从理论上说，用含有抗病或抗除草剂的基因进行试验，可能产生更难于对付的杂草。

Theoretically , experiments with genes that confer resistance to disease or herbicides could create hardier weeds .

抗除草剂Bar基因对小麦的遗传转化

Genetic Transformation by Transferring Herbicide Resistance Bar Gene into Wheat

所以，在本试验条件下和时间范围内，转抗除草剂Bar基因稻谷作为饲料对传代成年小鼠的健康无明显不良影响。

So , in this experimental condition and time range , herbicide resistant Bar gene rice as feed on the passage of adult mouse health without obvious adverse effect .

因此，在本试验条件下，转抗除草剂Bar基因稻谷喂养小鼠对其日行为和器官发育未产生明显的危害。

Therefore , under the conditions of this experiment , the Bar-Gene genetically modified rice feeding mice on the daily behaviors and organ development without apparent harm . 2 .

作者通过根癌农杆菌（Agrobacteriumtumefaciens）Ti质粒介导和基因枪法，将含抗除草剂Bar基因的植物表达载体，导入小麦幼穗和幼胚愈伤组织。

The plant expression vector carrying a herbicide resistance BAR gene and a marker GUS gene was transferred into wheat spikelets and immature embryo calli via Agrobacterium tumefaciens Ti plasmid and microprojectile bombardment .

4杂交育种以含Bar基因的抗除草剂小麦基因遗传工程体为基因供体，与安徽品种进杂交，获得的F1后代均为抗性植株。

Through the hybridization of Anhui local wheat cultivated variety and the herbicide resistance variety which is including Bar gene by genetic engineering , the herbicide resistance F1 generation plant were obtained which was self-crossed or backcrossed with Anhui variety .

用基因枪法将含有抗除草剂bar基因的植物表达载体导入优良品种小麦幼胚愈伤组织，并通过GUS瞬时表达对沉淀剂、轰击距离、金粉用量等轰击参数进行了优化。

The plant expression vector carrying the herbicide resistance bar gene was introduced into wheat immature embryo calli using particle bombardment . Parameters of bombardment were optimized by GUS transient expression , such as precipitants , distance , and amount of gold powder .

加拿大已经在野生白菜种群中发现了抗除草剂外源基因。

Herbicide resistant transgene has been found in wild B. rapa population in Canada .

转基因抗除草剂油菜相关基因的PCR检测及其在育种中的应用

PCR Detection of Herbicide-resistance-related Genes in Transgenic Rapeseed and Its Application in Breeding

种植于北达科他州的转基因油菜导入了能够使它们拥有抗除草剂能力的基因，包括“杂草杀手”Roundup。

Transgenic canola plants in North Dakota had received genes making them resistant to , such as the weed killer Roundup .

抗除草剂转基因作物基因流及其安全性评估方法的探讨

Safety assessment on gene flow of herbicide-resistant transgenic crops

富阳地区稻田杂草及抗除草剂水稻99-1bar基因漂移评价研究

Weed Survey in Fuyang Paddy Fields and Bar Gene Flow of Herbicide Resistant Rice 99-1

针对目前抗除草剂转基因作物基因流及其安全性评估方法存在的问题，提出了以亲和性为依据的抗性基因流的评估方法；

Aiming at existing problems with methods for safety assessment of herbicide-resistant transgenic crops , a new method based on affinity of plants for safety assessment of gene flow was put forward .