抗人球蛋白试验

结果：1.阳性率：经典的抗人球蛋白试验法为16.17%；改良的抗人球蛋白试验法为17.49%，SPSS13.0统计学软件分析，两者的差异无统计学意义（P0.05）。

The positive rate : classical coombs test is 16.17 % , the improved coombs test is 17.49 % . There is no statistic significant of these two methods by the SPSS 13.0 software . 2 .

25例行抗人球蛋白试验，3例阳性（120%）；

In 25 patients with SLE receiving Coombs test , 3 were positive ( 12.0 % ) .

18例SLE患者行抗人球蛋白试验，阳性率为66.7%。

18 cases were received Coombs test , the positive rate was 66.7 % .

Rh因子及抗人球蛋白试验方法简便、易行，一般医院均可进行。

The methods for examination of coombs and the D factor of Rh are simple and liable to practice , available in nearly all of hospitals .

方法：采用微柱凝胶抗人球蛋白试验分别测定妊娠16周、28~32周、36~38周的孕妇血清中IgG血型抗体效价。

Methods : The titers of blood group antibody IgG of pregnant women 's serum at 16th , 28 ~ 32nd , 36 ~ 38th week were detected by microcolumn gel immunology assay .

目的通过直接抗人球蛋白试验（DAT）阳性的类型鉴别，有助于临床对自身免疫性溶血性贫血（AIHA）的正确诊断；

Objective To distinguish types of the direct antiglobulin test ( DAT ) positive , help clinical to diagnose exactly the Autoimmune Hemolytic Anemia ( AIHA );

方法用DIANA全自动血型仪对23例AIHA患者进行ABO、Rh（D）血型常规定型及氯喹放散实验后定型，同时进行直、间接抗人球蛋白试验。

Methods The blood samples of 23 patients with AIHA were identified by routine typing and typing after chloroquine elution test on DIANA auto instrument . Direct and indirect antiglobulin test were done in the same time .

方法DAT阳性标本分AIHA组和非AIHA组，进行免疫分型，然后把IgG或IgG+C3的标本进行放散试验，利用放散液与标准抗体筛查细胞进行间接抗人球蛋白试验（IAT）。

Methods DAT positive samples can be divided into AIHA group and non AIHA group , process immunity types , and test the samples of IgG or IgG + C 3 with elution , use elution liquid and standard antibody screen cell to process indirect antiglobulin test ( IAT ) .

直接抗人球蛋白试验阳性的类型鉴别及临床意义

Distinguish types and clinical significance of direct antiglobulin test positive

考察骨髓单个核细胞直接抗人球蛋白试验（BMMNC-Coombs）的敏感性。

2 ) To evaluate the sensitivity of bone marrow mononuclear cell ( BMMNC ) Coombs tests .

三项试验中直接抗人球蛋白试验阳性49例（39.2%），释放试验阳性110例（88%），游离试验阳性87例（69.6%）；

The positive rates of direct antiglobulin test ( DAT ), elution test and indirect antiglobulin test ( IAT ) were 39.2 % , 88 % and 69.6 % respectively .

作者认为，生后24小时发生高胆由多种因素所致，与脐血胆红素值、于血症、改良直接抗人球蛋白试验关系较大。

The authors propose that the appearance of HPB correlates chiefly with the following factors : such as , the level of umbilical bilirubin , neonatal blood group , and the result of modified direct antiglobulin test .

三项血清学检测中最有诊断意义的试验为抗体释放试验，阳性率高达100%，改良直接抗人球蛋白试验阳性率为42.9%。

Antibody release test was of the most important significance in diagnosis of ABO hemolytic disease , the positive rate of which was 100 % , and the positive rate of the direct anti-globulin test was only 42.9 % .

方法：采用微柱凝集抗人球方法和传统抗人球蛋白试验检测120例孕妇血清IgG抗体效价，并对两种方法的结果进行方法学评价。

Method : The serum IgG valence of antibody in 120 pregnant women were test by microcylinder agglutinative and traditional antihuman globulin test respectively , the results were evaluated .