免疫筛选

cDNA文库的免疫筛选

Immunoscreening of cDNA libraries

旋毛虫成虫cDNA文库免疫筛选及序列分析

Immunoscreening and Sequence Analysis of a cDNA Library of Adult Trichinella spiralis

大鼠感染血清免疫筛选弓形虫速殖子cDNA文库

Immunological Screening of Toxoplasma Tachyzoite cDNA Expression Libraries with Serum from Infected Rats

免疫筛选弓形虫速殖子cDNA文库

Immunological screening of Toxoplasma tachyzoite cDNA expression library with sera from immune rabbits

常氏肝癌细胞cDNA文库的构建及ADAMs相关基因的免疫筛选与序列分析

Construction of Chang Liver cDNA Library and Immunoscreening of ADAMS Related Genes

旋毛虫3日龄成虫cDNA文库的免疫筛选

Immunoscreening of cDNA Library of Adult Trichinella spiralis

猪囊尾蚴cDNA表达文库的免疫筛选

Immune screening of cDNA library from Cysticercus cellulosae

GFAP基因克隆的免疫筛选对人脑胶质瘤cDNA基因文库质量的鉴定

Qualitative Identification of the Human Glioma cDNA Library by Immunoscreening of GFAP cDNA Clones

日本血吸虫未成熟卵抗血清对日本血吸虫cDNA文库的免疫筛选

Immuno-screening of Schistosoma japonicum adult worm cDNA library by the sera of anti & immature egg 26 / 28 kDa antigens

目的通过构建日本血吸虫成虫cDNA表达文库，免疫筛选全长目的基因，研究其结构和功能。

Objective To construct a cDNA expression libraries of adult schistosoma Japonicum for screening the genes and studying the structure and function of genes .

通过B.gibsoni感染狗血清对由B.gibsoni裂殖子构建的cDNA基因表达文库进行免疫筛选，其中获得的27个阳性克隆是编码同一蛋白的cDNA。

A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum . Twenty-seven repeat positive cDNA clones were obtained .

本文即在构建草地螟中肠cDNA文库的基础上，利用围食膜蛋白的多克隆抗体免疫筛选来获得编码相关基因，并进行了初步的分离与鉴定。

Based on the constructed cDNA library of the meadow moth midgut , we used PM proteins polyclonal antibody to isolate and characterize the related coding genes .

结论成功构建高质量人源性大肠癌cDNA噬菌体表达文库，适合于免疫筛选cDNA克隆的人大肠癌相关抗原基因。

Conclusion The successful construction of the human colorectal carcinoma cDNA phage library may have an important role in the searching for human colorectal carcinoma associated antigen genes through immunoscreening cDNA clones .

以上结果表明，制备的玉米赤霉醇抗体适用于对ZER残留的快速筛选检测，而MILO抗体有潜力用于构建MIL生产菌株的高通量免疫筛选模型。

The results showed that the antibodies for zeranol could be used for ZER residues analysis and MILO antibodies were potentially useful in construction of antibody models in high - throughput screening for MIL-producing strains .

日本血吸虫病患者血清对噬菌体随机7肽库的免疫筛选

Immunoscreening of phage random peptide library with sera from patients with schistosomiasis japonica

以此血清对表达文库进行了三次免疫筛选，最终获得5个阳性菌落。

The expression library was screened three times . Ultimately five positive colonies were found .

目的从噬菌体随机12肽库免疫筛选出大鼠对血吸虫天然抗性的模拟靶表位，探讨其抗日本血吸虫的免疫保护效果。

Objective To obtain short peptides simulating antigenic epitopes related with natural resistance to Schistosoma japonicum ( S.j ) in rats , and to explore their immune protective immunity against S.j in mice .

日本血吸虫尾蚴抗原免疫血清筛选成虫cDNA文库与新基因分析

Immunoscreening of Schistosoma japonicum Adult Worm cDNA Library with Sera Vaccinated with Cercaria Antigen and Analysis of Novel Genes

ELISA法操作简便快速，结果判读客观明确，优于传统的IHA法，可作为人破伤风类毒素免疫血浆筛选的常规方法。

ELISA can be rapidly and simply operated and the result can be directly and clearly determined . It is superior to IHA that ELISA could be used as a routine method for screening human antiserum for tetanus toxoid .

新的免疫调节剂筛选模型的建立

The Establishment of a Screening Method for Immunoregulators

采用激光扫描荧光成像技术，对基于亚毫米级微球的免疫荧光筛选和荧光成像进行了探索。

Applying Ar + - laser fluorescence scan-imaging technology , immunofluorescence screening and fluorescence imaging on sub-millimeter class microbeads are explored successfully .

免疫共沉淀筛选细胞内与A型流感病毒M2蛋白相互作用的蛋白

Screening cellular proteins interacted with M2 protein of influenza A virus by Coimmunoprecipitation

抗日本血吸虫病天然分子疫苗免疫猪血清筛选日本血吸虫尾蚴cDNA文库结果分析

Screening of Schistosoma japonicum Cercaria cDNA Library Using the Schistosoma japonicum Natural Molecular Vaccine Induced Immune Sera

经间接ELISA和间接免疫荧光试验筛选，共获得了15株NDV杂交瘤细胞。

After screening by indirect ELISA and IFA , 15 MAbs were obtained .

用单向火箭免疫电泳法筛选健康献血员，获得LP（a）阳性新鲜血浆。

Healthy blood donors were screened LP ( a ) by single electroimmunoassay .

将无细胞病毒免疫小鼠后筛选获得6株可特异性结合GlycoproteinK（gK）抗原的单克隆抗体。

Six monoclonal antibodies specifically binding Glycoprotein K ( gK ) were obtained by mice immunization of cell-free virus .

方法采用活细胞ELISA和免疫印迹法筛选含高滴度抗黑素细胞抗体的白癜风患者血清。

Methods Sera were screened for the presence of a high titer of antibodies against melanocytes from 256 patients with vitiligo by cellular enzyme-linked immunosorbent assay ( cellular ELISA ) and Western blotting .

方法通过RNA免疫沉降法筛选抗U1RNP抗体阳性血清，免疫印记分析检测血清中抗HPAEC自身抗体。

Methods Sera with anti-U_1RNP antibodies ( Abs ) were selected through RNA immunoprecipitation assay , and autoantibodies against autoantigens of HPAEC were detected by immunoblot .

同时采用荧光素酶双报告基因方法检测了Wnt通路活性，用免疫共沉淀筛选作用于Wnt通路的关键蛋白。

The Wnt pathway activity was detected by luciferase dual reporter gene assay , and the target protein in Wnt pathway was revealed by immunoprecipitation .

体内诱导抗原的筛选：用免疫印迹方法筛选经过IPTG诱导表达的文库，经过两次筛选后得到39个阳性克隆。

Screening the in vivo induced antigens : We screened the IPTG induced expression library by western blot , 39 positive clones were acquired after twice screening .