免疫荧光抗体

直接免疫荧光抗体试验和RT-PCR检测，9头均为阳性。

The results of 9 piglets of HCFA and RT PCR were positive .

并采用免疫荧光抗体染色技术对5例DMD，2例BMD肌细胞膜上抗肌营养不良蛋白的表达观察分析，以2例正常人的肌组织作为对照。

The patients from 5 DMD and 2 BMD were detected by immunofluorescence technique for analyzing dystrophin located in muscle cell membrane , compared with 2 normal males .

7株杂交瘤培养上清的免疫荧光抗体（IFA）滴度为1：32～256，腹水IFA滴度为1：8000～25600。

The antibodies titres of culture fluids from 7 hybridoma cells were 1:32-256 , and those of mouse ascites were 1:8000-25600 by indirect fluorescent antibody technics ( IFA ) .

结果外周血单个核细胞在体外诱导分化成为内皮祖细胞，ac-LDL吞噬及lectin抗体荧光标记双阳性，FLK-1和vWF免疫荧光抗体染色均为阳性。

Results The peripheral blood mononuclear cells differentiated into EPCs . They were positively labeled by fluorescent acetylated-LDL , lectin , FLK-1 and vWF .

吖啶橙染色则使Cpn感染的Hep-2细胞呈现鲜明的橘红色；免疫荧光抗体染色后，在Cpn感染细胞内可见亮苹果绿色包涵体。

It also showed Cpn infected Hep-2 cell would turn amethyst or magenta using Romanowsky-Giemsa staining , bright orange using AO staining and apple green cytorrhyctes using DI staining .

本文以直接免疫荧光抗体法（DFA）检测150例门诊就诊妇女的生殖道沙眼衣原体感染，总检出率为8%。

This paper reported a study of chlamydia trachomatis ( CT ) infection of reproductive sys-tem in 150 out-patients by using DFA - The total positive rate was 8 % .

猪生殖和呼吸综合征间接免疫荧光抗体试验诊断技术的研究

Studies on IFA diagnostic technique for porcine reproductive and respiratory syndrome

间接免疫荧光抗体法快速检测脓液中的厌氧菌

Indirect Fluorescent Antibody Assay for the Rapid Detection of Anaerobes in Clinical Specimens

间接免疫荧光抗体法对鸡传染性支气管炎病毒进行抗原定位

Localization of Avian Infectious Bronchitis Virus by Indirect Immuno - Fluorescent Antibody Plan

间接免疫荧光抗体试验在传染性非典型肺炎诊断中的应用

The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome

结果经人第Ⅷ因子免疫荧光抗体检查法鉴定，脐带血管内皮细胞经原代培养后获得人脐静脉血管内皮细胞系。

Results After antihemophilic factor antigen was tested by immunofluorescence test , The HUVEC cell line was identified .

目的制备可用于临床检测阴道加德纳菌的免疫荧光抗体。

Objective The preparation is an immunofluorescence antibody which can be used for determining of clinical Gardnerella Vaginalis .

方法：采用间接免疫荧光抗体法和直接荧光抗体染色法，于1999～2000年在平江县选点调查。

Methods : By means of IFA and DFA from 1999 to 2000 serological and epidemiological investigations were conducted .

用间接免疫荧光抗体法，对鼠体内两种类型卫氏并殖吸虫虫体抗原与宿主抗原做了检测。

The detection of somatic antigens and host antigens in two types of juvenile of P. westermani was performed in IFA .

试验结果表明：水貂的脾脏、肝脏和心脏可作为直接免疫荧光抗体检测的首选器官。

The experimental results showed that the spleen , liver and heart could be the primary organs for the direct fluorescent antibody test .

对各种病原鳗弧菌的检测方法有很多报道，主要有免疫荧光抗体技术和核酸杂交技术等，但是免疫荧光抗体技术需要制备抗血清，核酸杂交技术灵敏度只有150pg。

Lots of method are reported for the detection of pathogenic Vibrio anguillarum , for example , fluorescent antibody technique , nucleic acid hybridization .

目的：调查山东省沂蒙山区莱姆病自然疫源地。方法：1992～1999年设点，用间接免疫荧光抗体试验进行人群莱姆病感染调查；

From 1992 to 1999 an endemic investigation of Lyme disease was conducted by means of serological and etiological methods in Yimeng mountains of Shandong province .

结论人脐静脉血管内皮细胞可从脐带获得并通过原代培养成为细胞系，而人第Ⅷ因子免疫荧光抗体检查法是鉴定人脐静脉血管内皮细胞的良好方法。

Conclusion HUVEC cell line can be got from infant umbilical cord by primary cultivation , and antihemophilic factor antigen tested by immunofluorescence test is a good method for identification .

免疫荧光检测抗体的表达，竞争抑制ELISA检测其与抗原的结合活性及特异性。

The expression of anti-HBs human IgG was tested with blocking ELISA and immunofluorescent test .

方法：采用直接免疫荧光单克隆抗体法（DFA）检测诊断。

Methods : The pathogens were detected by using direct immunofluorescent assay of monoclonal antibody ( DFA ) .

在洽疗后2、4日，对照组的特异性免疫荧光IgM抗体明显高于研究组的。

The specific immunofluorescent IgM antibody in the control group was significantly higher than that in the study group on day 2 and day 4 after the treatment ;

用免疫荧光单克隆抗体对脊髓灰质炎病毒抗原表位的分析

Analysis of Poliovirus Antigenic Epitopes by Immunofluorescence Activated McAbs

运用免疫荧光检测抗体的功能发现，无分泌能力的抗体，其中和抗原的能力也明显弱于正常分泌的抗体。

Fluorescent analysis was further used to test the antibody functions , which showed the binding capacity of the 2 secretion defect antibody were significantly weaker than normal secretion antibodies .

目的探讨免疫荧光和双抗体夹心ELISA在人狂犬病检测中的应用。

Objective To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies .

免疫荧光检测HIV-1抗体方法的建立和应用

The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1

血清间接免疫荧光试验，抗体滴度1∶512。经用大剂量青霉素静脉注射症状消失。

The antibody titre in the serum rose to1 ∶ 512.He was cured with large doses of penicillin .

疫苗经1：10或1：30稀释后免疫家兔，荧光抗体阳转率仍达100%，但抗体滴度明显降低。

Rabbits immunized with the vaccine at 1:10 or 1:30 dilution shoved 100 % seroconversion rate of IF antibody two weeks after the first injection , but the antibody titres were lower .

间接免疫荧光技术检测狂犬病抗体IgG

Indirect immunofluorescent antibody technique to detect rabies antibody IgG

在80份流行性出血热病人及对照病人血清标本中，血凝抑制抗体（HI）和免疫荧光（IF）抗体的滴度间呈显著的相关。

The level of immunofluorescent ( IF ) and HI antibody were significantly correlated in sera of patients and controls .

牛舌为底物间接免疫荧光检测天疱疮抗体的研究

Study on pemphigus antibodies with indirect immunofluorescence by using bovine tongue as substrate