合成寡核苷酸

结论：采用PCR的方法，实现合成寡核苷酸片段的一次性组装，方便了进一步的克隆。

Conclusion : The synthetic oligonucleotide fragments were assembled in only one step by a PCR approach , which is convenient to be further cloneed .

通过合成寡核苷酸和PCR技术将SCFcDNA中的原核稀有密码子定点突变为同义的高频密码子；

The prokaryotic rare codons in SCF cDNA sequence were mutated into high frequency synonymous codons by method of PCR .

根据保守区各已知病毒的cDNA特异序列合成寡核苷酸，将相同区的寡核苷酸等量混合成为鸡尾酒引物，用于RT-PCR。

Oligonucleotides were synthesized according to the cDNA sequences at the conservative regions . Then the oligonucleotides of the same region were mixed together as " cocktail " primers for RT-PCR .

合成寡核苷酸对病毒目标序列特异结合反应及其影响因素研究

Specially binding of synthesized oligonucleotide to target viral gene sequences and the influencing factors

方法：应用生物素标记的人工合成寡核苷酸探针，对72例心肌石蜡包埋组织切片进行原位核酸杂交。

Methods : Hybridization in situ was performed by the use of the oligonucleotide probe by artificial synthesis labelled with biotin .

借助从人体胎盘纤维细胞bFGF受体序列合成的寡核苷酸引物，聚合酶链反应体外扩增cDNA。

CDNA segments were amplified by polymerase chain reaction using bFGF receptor oligonucleotide primer from sequence of fibroblast cells in human placenta .

本实验的第一部分，首次以草鱼体外培养的头肾巨噬细胞为模型，探讨了多种人工合成的寡核苷酸序列和两种不同细菌的基因组DNA对巨噬细胞活化的作用。

In Part I , we used head kidney macrophages of grass carp ( Ctenopharyngodon idellus ) as an in vitro model and investigated the effects of several CpG-ODNs , Escherichia coli DNA and Bifidobacterium adolescentis DNA on fish immunocytes .

采用DEAE-52纤维素小柱，纯化了7个人工合成的寡核苷酸引物。

Seven synthesised oligonucleotide primers were purified with a small column of DEAE-52 cellulose at our laboratory .

用合成的寡核苷酸探针鉴定中国人β-地中海贫血基因突变

Use of Synthetic Oligonucleotides in the Detection of β - thalassemia Mutation in Chinese

本文用人工合成的寡核苷酸探针，经斑点杂交法检测蚊体内马来丝虫幼虫。

The synthesized oligonucleotide probe was used in Dot hybridization to detect B.malayi filarial larvae from mosquitoes .

应用合成的寡核苷酸作杂交探针来检测β地中海贫血基因，在3个患病家庭中鉴定出5种不同的点突变型。

The synthetic oligonucleotides were used as hybridization probes to detect the β thalassemia genes , and 5 different point mutations in 3 affected families were identified .

用合成的寡核苷酸引物从中扩增了抗体的轩、重链可变区基因，并克隆入质粒.随机挑取阳性克隆各一个进行核苷酸序列分析。

Variable region genes of the antibody light and heavy chains were amplified from the cDNA with pairs of synthetic oligomers as primers , cloned , and sequenced .

采用适合批量生产的亚磷酰胺方法合成反义寡核苷酸（5′cgatggcacggcgcactt3′）。

ODN ( 5 ' cga tgg cac ggc gca ctt 3 ' ) targeted to survivin mRNA was synthesized , we introduced a simple phosphoramidite method to produce ODN .

方法根据偶然分支杆菌16S23Srdna间隔区序列，设计合成一段寡核苷酸探针，采用PCR扩增、RFLP和DNA斑点杂交技术，对29株偶然分支杆菌临床分离株进行鉴定。

Methods A single pair of prime and oligonucleotide probe of M.fortuitum were designed , according to the sequence of mycobacterium of 16S-23S rDNA spacer sequences . 29 clinical strains were amplified by PCR , then dot-blot hybridization and RFLP of PCR products were made .

根据4种猪疫病病毒特异性基因的保守区域设计合成了60mer寡核苷酸探针，制备寡核苷酸芯片。

The 60-mer oligonucleotide probes based on specific conservative DNA fragments of the four porcine viruses were designed and synthesized , then applied to fabricating the oligonucleotide microarrays .

利用人工合成的一对寡核苷酸引物，采用PCR技术特异性地扩增hCG－β－cDNA。

We designed a pair of oligonucleotide primers to specifically amplify hCG - β cDNA .

SELEX技术是一种组合化学新技术，应用该技术可从人工合成的随机单链寡核苷酸文库中筛选出能与靶分子高特异性、高亲和力的核酸适配子。

SELEX is a kind of combinatorial chemistry technology , by which nuclear acid aptamers with high specificity and affinity to targets could be screened out from a random oligonucleotide library .

设计并合成了两对寡核苷酸引物，P1和P2用于扩增新城疫病毒（NDV）全长F基因，P3和P4用于扩增NDV部分F基因。

Two pairs of primers were designed and synthetized , in which the P 1 and P 2 were used to amplify the complete F gene of NDV , and the P 3 and P 4 to amplify the part of F gene ( 1 ~ 813bp ) .

化学法合成生物素标记寡核苷酸探针

Chemical Synthesis of th Biotinylated Oligonucleotide Probes