蛋白印迹法

方法采用蛋白印迹法检测了酪氨酸酶蛋白和干细胞因子受体kit蛋白含量的变化；

Methods The TRP and KIT protein expression was detected by Western blotting .

通过病理组织切片观察肝组织病理变化，并采用免疫蛋白印迹法（westernblot）测定小鼠肝匀浆中细胞因子表达。

Histological examination observed histopathology change of the livers , and cytokines in liver were detected using Western blot .

蛋白印迹法与免疫荧光法检测白血病患者Survivin表达

Expression of Survivin in leukemia by Western blot analysis and immunofluorescence method

Western蛋白印迹法和免疫组化法检测PC12细胞ERK1/2蛋白表达及定位情况。

The expression and localization of ERK1 / 2 were determined by Western blot and immunohistochemical methods .

方法用蛋白印迹法测定UVB辐射后，成纤维细胞原癌基因c-jun和c-fos的蛋白c-jun和c-fos的表达变化；

Methods The c-jun and c-fos protein expression induced by UVB were measured by Western blot .

阳性标本用蛋白印迹法（WB）做确认试验。

Confirmation tests on positive samples were done with protein Western Blot ( WB ) .

蛋白印迹法检测恒河猴SRV血清抗体的研究

Study on Detecting SRV Serum Antibodies in Rhesus Monkeys by Western Blot

方法用免疫蛋白印迹法检测原发性前列腺癌LNCaP细胞和由其起源的转移性前列腺癌C4-2细胞中VEGF的表达。

Methods Expression of VEGF of LNCaP cell in metastatic prostate cancer C4-2 cell , which derived from LNCaP cell .

免疫蛋白印迹法分析caspase-9、caspase-8、caspase-3、PARP、bcl-2、bax和胞浆细胞色素c的含量。

The expression of Caspase-3 , Caspase-8 , Caspase-9 , PARP , Bcl-2 , Bax and the concentration of cytochrome c in cytosol were analyzed by Western blot .

同时取各组皮肤样本提取Ⅰ型胶原蛋白，使用蛋白印迹法（westernblot）来检测各组样本中Ⅰ型胶原蛋白的表达有无差异。

At the same time to take the type I collagen in the skin samples , using the Western blot technique ( Western blot ) to detect the difference of whether the expression of type I collagen protein in the sample .

方法采取前瞻性对照研究方法，对202名健康者及40例经免疫蛋白印迹法（WB）确证的HIV/AIDS病人进行PPD试验、CD4、CD8淋巴细胞计数。

Methods The prospective study was applied . PPD , CD_4 and CD_8 cell counts were examined between 202 health and 40 HIV / AIDS patients confirmed by western blot .

方法：总结51例早期梅毒的临床表现，对RPR、FTA-ABS、蛋白印迹法以及PCR等多种诊断方法进行评价。

Methods : 51 cases of early syphilis were summarized , RPR , FTA ABS , Western blot and PCR techniques were compared in the diagnosis .

方法：5-FU造成离体人支气管环严重损伤，分别取正常组及5-FU作用后的支气管上皮，应用流式细胞仪和蛋白印迹法检测P-gp的表达；

Methods : The isolated human bronchial epithelium was injured by 5-FU . The expression of P-gp in 5-FU group and control group was detected by flow cytometry and Western Blotting .

方法应用DNA琼脂糖凝胶电泳检测胃癌细胞BGC-823凋亡，以蛋白印迹法检测PARP蛋白及活化片断表达情况。

Methods The agarose gel electrophoresis of DNA was used to detected apoptosis of gastric carcinoma cells BGC-823 , and the expression of the poly ADP-ribose polymerase protein and its activated fragment were detected by Western-blotting assays .

HE染色后图像分析检测大鼠气道形态学参数，免疫组化方法用于BMP-2，BMP-4表达定位，Western蛋白印迹法测定BMP-2，BMP-4含量。

The changes of airway , the expressions of BMP-2 and BMP-4 , and the contents of BMP-2 and BMP-4 were determined by using image analysis system , immunohistochemical method , and Western Blotting , respectively .

方法5F作用K562细胞24h后，以MBP为底物检测MAPK的活性、用蛋白印迹法测定MAPK的表达。

METHODS K562 cells were treated with compound 5F for 24 hours , then MAPK activity was determined using MBP as substrate , Western blotting was used to detect the effect of compound 5F on the expression of MAPK .

方法以4对8种试剂（同一厂家间接法和双抗原夹心法配对）对100份人血清样品分别进行对比实验，阳性结果以蛋白印迹法（WB）确认。

Methods Eight kits were divided into 4 groups according to the manufacturers ( indirect method and dual antigen sandwich method for the same manufacturer in one group ) and were used for the determination of 100 serum samples .

免疫组化、RT-PCR和蛋白印迹法研究在位内膜与培养基中ENDO-1mRNA及蛋白的表达，而ENDO-1可以改变腹腔巨噬细胞的功能，并能促进血管增生。

Study the expression of mRNA and protein of ENDO-1 in endometrium and culture supernatant with IHC , RT-PCR and western blotting . ENDO-1 is believed to be able to change the function of macrophages and promote angiogenesis . 4 .

应用免疫组织化学和固定化蛋白印迹法检测急性缺血后海马皮层MARCKS及p-MARCKS蛋白表达的改变以及中药的调节作用。

Changes in the expressions of MARCKS and p-MARCKS in hippocampal cortex experienced acute ischemia and the regulating effects of TCM drugs on their expressions were detected by using immunohistochemical method and Western blot method .

利用蛋白印迹法研究了小鼠精子在附睾成熟过程中质膜糖蛋白的变化。

Glycoprotein changes in mouse sperm plasma membrane during epididymal maturation were revealed by Western blot .

采用蛋白印迹法测定心肌细胞中热休克蛋白70含量。

The content of heat shock protein 70 in myocardium was detected with western blot method .

方法：取高、低体温大鼠下丘脑组织，应用蛋白印迹法测定热休克蛋白含量。

Method : Using Western blot method to measure HSP of hypothalamus in febrile and hypothermal rats .

应用蛋白印迹法检测鼻咽癌病人血清中抗Epstein&Barr病毒IgA/EA抗体

Detection of Epstein-Barr virus iga / ea antibody in sera from nasopharyngeal carcinoma patients by Western blot

通过免疫蛋白印迹法测定ERK1/2、p-ERK1/2蛋白的表达。

Western blotting was used to detect the expression of both ERK1 / 2 and p-ERK1 / 2 protein .

表明蛋白免疫印迹法是一项适合研究体液中Fn降解片段特性和发现新片段的方法。

Western blot is a method suitable to study the character of Fn fragment and detect new Fn fragment .

用蛋白免疫印迹法研究醋酸铅对PC-12细胞Bax、Bcl-2和p53蛋白表达的影响。

The expressions of Bax , Bcl-2 , and p53 in PC-12 cells after exposure to lead acetate were detected by Western blot .

蛋白免疫印迹法检测乙酰化组蛋白H3、H4、P300、P53、c-myc蛋白的表达。

The expression of acetyl histone H3 and H4 , P300 , P53 and c-myc protein were tested by western blot .

为了探讨NB诱导的凋亡与Bcr-Abl的关系，用蛋白免疫印迹法检测Bcr-Abl的含量。

In order to reveal the relationship between NB-induced apoptosis and Bcr-Abl , the amounts of Bcr-Abl protein were tested by Western blot .

方法：采用细胞免疫荧光法观察皂苷处理的CNE2Z细胞微管的变化，蛋白免疫印迹法分析皂苷处理的CNE2Z细胞中聚合和未聚合微管蛋白含量的变化。

METHODS : The interactions of tubeimoside with microtubule in CNE-2Z cells were investigated by immunofluorescence microscopy , and the interactions of tubeimoside with tubulin in CNE-2Z cells were detected by Western blotting .

蛋白免疫印迹法分析纤维连接蛋白分子降解状态

Analysis of the molecular degradation of fibronectin by Western blot