蛋白质转移
![蛋白质转移](../cache/1192199.jpg)
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westernblot是将蛋白质转移到膜上,然后利用抗体进行检测。对已知表达蛋白,可用相应抗体作为一抗进行检测,对新基因的表达产物,可通过融合部分的抗体检测。
Western Blot is that the proteins were transferred to a membrane , and then using the corresponding antibody to detect .
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根据EGFR及CD44的相对分子量选择PAGE凝胶区域进行转膜,将蛋白质转移到NC膜上。
PAGE gel area was selected to conduct trarsmembrane according to relative molecular weight of EGFR and CD44 , and then protein was transferred onto NC membrane .
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蛋白质转移电泳技术在单克隆抗体检测中的应用
Protein transfer electrophoresis for immunological screening of monoclonal antibodies
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针对该问题,本文在前人工作的基础上,开发出一种集凝胶中蛋白质转移和在线酶解的毛细管电泳方法。
In order to resolve this problem , based on the work of the formers , a capillary electrophoresis method involving gel protein transfer and on-line proteolytic digestion was developed .
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用蛋白质印迹转移技术分析正常及硒性白内障大鼠晶状体及房水中蛋白质的性质
Western Blot Analysis of Lens Proteins and Aqueous Humor in Normal and Cataractous Rat Eyes
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高温弱光下,四个品种剑叶的蛋白酶活性一致升高,高温弱光有利于叶片内的蛋白质降解转移。
Under the condition of high-temperature and weak-light , the protease activity of the flag leaf all increased in the four kinds of rice , that means high-temperature and weak-light was conducive to the transfer of the protein degradation .
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这样的转移不会引起气泡,可以大大提高实验的成功率及重复性,有利于蛋白质的转移(尤其是相对分子质量大于40kDa的蛋白质),提高质谱鉴定的准确性。
The improved transfer method will not induce air bubbles , so it can improve the chance of success and reproducibility , convenient for the transfer of proteins ( especially of the higher molecular mass than 40 kDa proteins ), and improve the accuracy of the identification by MS.
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亚甲蓝修饰电极推动的血红素蛋白质直接电子转移反应
Direct Electron Transfer Reaction of Hemeproteins Promoted by Methylene Blue Modified Electrode
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所有的病毒都无法在细胞内复制,因为这些细胞内的一种蛋白质(甲基转移酶SETD3)的指令已经关闭。
All the viruses were unable to replicate inside cells which had the instructions for a protein ( called methyltransferase SETD3 ) switched off .
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基于蛋白质直接电子转移的全胆固醇传感器硅橡胶膜生物反应器乙醇连续发酵传质动力学实验研究
Total Cholesterol Biosensor Based on Direct Electrochemistry of Proteins Mass Transfer Kinetics of Pervaporation in A MBR with Composite Silicone Rubber Membrane for Continuous Ethanol Fermentation
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并得出除蛋白质Z、脂转移蛋白外,醇溶蛋白残片也应是泡沫蛋白的一个重要组成。
And it was concluded that alcohol soluble protein relics were also an important composition of foamy protein except protein Z and lipid-transferred proteins .
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微生物谷氨酰胺转胺酶(MTG)可通过催化蛋白质谷氨酰胺的酰基转移反应改变蛋白质的结构和性质,是一种有广泛应用前景的重要新型酶制剂。
The enzyme of microbial transglutaminase ( MTG ) can modify the structure and properties of proteins through catalyzing an acyl transfer reaction of a γ - carboxyamide group of glutamine residue in a peptide chain , resulting the cross-linking of proteins .
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着重论述了林木营养贮藏蛋白质的合成、转移、降解机理及基因表达与调控等方面的最新研究进展;
This paper reviews the results of research on localization , identification and major characterization of vegetative storage proteins in woody plants .
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所得模型正确地反映了含蛋白质的逆胶束系统稳定的原因,较好地描述了蛋白质转移后胶束大小的变化以及蛋白质转移率随电解质浓度变化的特殊的实验规律。
The model presented can properly describe the size change of reverse micelles after the protein uptake . The experimental results of the protein transfer ratio as a function of salt concentrations can also be reproduced satisfactorily .