蛋白质设计

该工作为基于相对熵及HNP模型的蛋白质设计研究打下了基础。

A foundation for studying protein design using the HNP model and the relative entropy was made .

基于结构生物信息学的蛋白质设计

Structural Bioinformatics Based Protein Design

蛋白质设计和蛋白质结构预测是紧密相关的。

Structure prediction and protein design are closely related .

四种结构类型的蛋白质设计方法

An approach to design proteins of four structural classes

为了与基于相对熵方法的蛋白质设计工作进行统一，采用了新的接触强度函数。

A new contact intensity function is given to consist with protein design research based on the relative entropy .

本文将两种蛋白质设计策略的特点、各自使用的方法、进化路线及最近三年在酶工程改造上取得的成就加以总结。

This article summarized the characteristics , evolution approach , evolution routines and the successful examples of the two protein modification strategies .

这些研究为基于相对熵的蛋白质设计方法的应用和进一步的发展打下了良好的基础。（2）从两个方面对基于相对熵的蛋白质结构预测方法进行了改进。

All analyses mentioned above make the foundation for the development and the application of this method in the future . ( 2 ) We have modified the relative-entropy-based method from two aspects as follows .

为了克服双重蒙特卡罗方法用于较大分子体系及非格子模型的困难，提出了一个基于相对熵的蛋白质设计的简单方法。

In order to overcome the difficulty of applying dual Monte Carlo method to the bigger molecule system and off-lattice model , a simple new method of protein design based on relative entropy is proposed .

表达的融合蛋白质通过设计的特异性位点的酶切得到重组的人胰岛素原C肽单体。

Coli and the recombinant proinsulin C peptide was produced through site specific cleavage of the resulting gene products .

蛋白质核心设计的序列组合文库筛选方法

Design of Protein Cores by Screening Combinatorial Sequence Library

用于蛋白质分子设计的环区模建方法

Modeling Method of Loop for Protein Molecular Design

特异性界面的设计是蛋白质功能性设计的主要问题之一。

It is an important issue in protein functional design .

用蛋白质工程方法设计和改造工业用酶

Design and reform of industrial enzymes by protein engineering

理解主宰蛋白质折叠和设计的规则将有助于这个问题的解答。

Understanding the rules that govern folding and design may help answer this question .

论文的第二部分是进行两类实际蛋白质的抑制剂设计。

The next part of dissertation is to design ligand for specific target proteins .

合成编码蛋白质基因的设计

Design of synthetic gene coding for protein

基于相对熵和复杂网络方法的蛋白质折叠与设计理论研究

Theoretical Study of Protein Folding and Protein Design Based on the Relative Entropy and Complex Network Methods

利用结构生物信息学方法进行蛋白质的理性设计，将会在后基因组时代的生物学研究和蛋白药物开发中起到越来越重要的作用。

Structural bioinformatics based rational protein design will play important role in biological research and development of protein therapeutics in the post genome era .

耦联性信息的提取还有助于指导同一家族的新蛋白质序列的设计，或者修改已有的序列提高其可折叠性和热稳定性。

Extracting of coupling information for different protein fold would do help to design new sequence of that family or to modify existing sequence to improve its fold-ability and / or thermostability .

基于nr本地化数据库的蛋白质注释系统的设计

Designing a Protein Annotation System Based on Local nr Database

本文以牛生长激素基因为例，对合成编码蛋白质基因的微机设计原理进行了探讨。

The strategy for designing synthetic gene with computer was described , and the computer program was compiled with advanced BASIC language .

新的抗体连接方法是为蛋白质印迹法专门设计的。后者是一种凝胶电泳法可根据细胞和组织样本的分子重量对其进行分离。

The new antibody conjugates are designed for Western blotting applications , which use gel electrophoresis to separate proteins from a sample of cell or tissue based on molecular weight and size .

蛋白质折叠相关研究对蛋白质设计、疾病成因等方面研究有决定作用，已经成为众多相关学科研究中的核心问题。

Research of protein folding becomes the core subject related to protein design and molecular biology .

结构基因组计划的实施使越来越多的蛋白质结构得到阐明，为进行蛋白质设计提供了更多的素材。

The Structural Genomics Project has disclosed crystal structures of more and more proteins .

所以蛋白质结构的研究是功能研究和蛋白质设计的基础，也因此越来越得到重视。蛋白质种类繁多，结构千差万别，同时蛋白质结构测定实验是非常费时费力，消耗资源也大，而且成功率有限。

Although researches on the protein structures got more and more attention because they are the foundation of the function research and protein design , the experiment of protein structural determination is quite time - and money-consuming and of limited success rate .

蛋白质折叠问题包含两个方面的内容：正折叠问题（称为蛋白质折叠）和逆折叠问题（即蛋白质设计）。

The protein folding problem has two components : the direct folding problem ( i.e. folding ) and the inverse problem ( i.e. protein design ) .

了解该机制将加深我们对蛋白质自组装过程的认识，进而为治疗各种蛋白质折叠病（如疯牛病和老年痴呆症）提供帮助，为蛋白质分子设计提供指导。

Understanding this mechanism would greatly accelerate our study on the protein self-assembling , and moreover , be helpful to protein design and to the treatment of various diseases relevant to protein misfolding ( such as mad cow disease and Alzheimer disease ) .

将一个蛋白质的活性位点，嫁接到另一个分子量较小但是稳定的蛋白质（骨架蛋白）上，从而形成一种新的功能蛋白质，这是蛋白质设计中一种很有效的方法。

Engineering novel small functional proteins by grafting active sites into small but stable proteins is an efficient protein design method .