志贺菌
- 网络shigella;shigellae;Shigella spp
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沙门菌和志贺菌二重PCR检测方法的建立及应用
Establishment and Application of Double PCR Detection Method for Salmonella and Shigella
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目的:探讨复方中成药克痢痧对志贺菌R质粒的消除作用,使其重新恢复对抗生素的敏感性。
Objective : Probe into the elimination effect on R plasmids from Shigella by compound Chinese medicine Kelisha , thus to restore the sensitivity to antibiotics .
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志贺菌6种毒力基因的多重PCR检测
Detection of six kinds of virulence genes in Shigella species by multiplex PCR
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沙门菌、志贺菌、副溶血性弧菌多重PCR检测方法的研究
Multiple PCR Detection Method for Salmonella spp. , Shigella spp. , and Vibrio parahaemolyticus
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方法对43株F2a志贺菌进行质粒图谱和质粒DNA酶切图谱分析。
Methods Plasmid profile and plasmid DNA restriction endonuclease analyses were carried out on 43 strains of S.flexneri 2a .
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对rrrA基因83位C-T的点突变,设计错配引物,以错配PCR的方法检测福氏志贺菌的耐药突变。
To fixed point mutant , mismatch primer was designed and Shield flexneri were detected by mismatch PCR .
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福氏2a志贺菌菌痢爆发的质粒DNA多态性分析
Plasmid DNA polymorphism analysis in an outbreak of S.flexneri 2a
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目的鉴定、分析新出现的福氏志贺菌4型流行菌株的表型和DNA型别特征。
Objective To identify and analyse the phenotypic and DNA genotypic characterization of a kind of new epidemical Shigella flexneri type 4 strains .
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儿童福氏志贺菌TEM型β内酰胺酶耐药基因及其耐药性检测
Detection of TEM-type β - lactamase gene and antimicrobial resistance in Shigella flexneri isolated from children
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对氨苄西林-舒巴坦也有一定的耐药,TEM基因阳性志贺菌、TEM基因阴性志贺菌的耐药率分别为41.2%、20.6%。
About 41.2 % of TEM-gene positive S.flexneri and 20.6 % of TEM-gene negative S.flexneri were resistant to ampicillin-sulbactam .
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志贺菌对HeLa细胞侵袭力的研究
Study on Invasiveness of Shigella to HeLa Cells
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抗菌膜单克隆抗体对志贺菌入侵HeLa细胞的影响
Influence of Monoclonal Antibody Against Membrane Component on the Invasion of HeLa Cells by Shigella
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福氏2a志贺菌DNA转录激活蛋白MarA的二级结构及其B细胞抗原表位预测
Prediction of the secondary structure and B cell epitopes for the DNA-binding transcriptional activator protein MarA of Shigella flexneri 2a
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目的对福氏2a志贺菌质粒DNA进行基因分型,提高福氏2a志贺菌菌痢爆发流行时同源克隆的鉴定水平。
Objective To identify homogenous clones of S.flexneri 2a by gene distribution polymorphism of plasmid DNA of S.flexneri 2a .
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46株intⅠ1阳性福氏志贺菌ant(3〃)-Ⅰ基因均阳性;
46 strains of intI positive Shigella flexneri were all positive for ant ( 3 ″) - ⅰ .
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福氏2a志贺菌磷酸烯醇丙酮酸盐合成酶基因的克隆与序列分析
Cloning and sequencing of phosphoenolpyruvate synthase ( pps ) gene of Shigella flexneri 2a
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以鸡鲍氏志贺菌、鸡白痢沙门菌和痢疾志贺菌为研究对象,分别提取基因组DNA,利用6条随机引物以随机扩增多态性DNA(RAPD)技术对其基因组DNA进行了分析。
The genetic diversity among strains of chicken Shigella boydii , Shigella dysenteriae and S.Pullorum was studied by random amplified polymorphic DNA ( RAPD ) with 6 random primers .
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从细菌分布的整体情况看,鸡志贺菌病病程在攻毒后的第2d达到感染最高峰,之后逐渐向健康转归。
From the whole bacterium distribution , the course of chicken shigellosis reachs to the peak of infection , later , go health gradually .
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福氏2c(新菌型)和福氏2a志贺菌生物学关系实验研究
Study on the biological relationship between Shigella Flexneri 2c ( a new serotype ) and 2a
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福氏2a志贺菌肠毒素ShET1/ShET2基因分型在菌痢暴发研究中的应用
Application of Shigella Enterotoxin 1 and Shigella Enterotoxin 2 Gene PCR Analysis in Outbreak of S.flexneri 2a
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方法对在菌痢暴发期间分离的8株福氏2a志贺菌用PCR法检测志贺菌肠毒素ShET1/ShET2基因,进行基因分型和同源克隆鉴定。
Methods Eight strains of S.flexneri 2a were detected by polymerase chain reaction to identify genetic type and homogenous clones of S.flexneri 2a .
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福氏2a志贺菌MarA蛋白纯化复性及活性检测
Renaturation , purification and identification for biological activity of shigella flexneri 2a MarA protein
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在临床上,直接用PCR方法检测病料增菌液中的鸡源志贺菌,可以节省诊断时间,为及时治疗提供保障。
In clinical , using PCR detection method to detect whether disease material culture has Shigella isolated from chicken can save diagnosis time , providing guarantee for timely treat .
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结论:新发现的福氏志贺菌2c与福氏志贺菌2a在药物敏感性上存在较大的差异。
Conclusion : There were some differences of antibiotic susceptibility testing between shigella flexneri 2c and 2a .
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福氏志贺菌4c亚型的药物抗性观察
Study on drug resistance of shigella flexneri 4c
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结果在检出的112株痢疾志贺菌(以下简称痢疾杆菌)中,B群103株(福氏痢疾杆菌)、D群9株(宋内痢疾杆菌)。
The confirming test were made for the suspicious strains . Results Of the 112 strains of Shigella detected , 103 were group B ( Shigella flexneri ) and 9 group D ( Shigella sonnei ) .
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弗氏志贺菌4c和2a型菌株的脉冲场凝胶电泳分型特征
Molecular Typing of Shigella flexneri 4c and 2a Isolates from Diarrhea Surveillance by Pulse Field Gel Electrophoresis
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福氏志贺菌感染后大鼠肠功能紊乱模型建立以及肠道通透性变化和肠黏膜IL-1β表达
Establishment of a Rat Model of the Post-dysenteric Bowel Dysfunction , Then the Change of Gut Permeability and IL-1 β in the Gut Mucosa
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天津地区志贺菌产ESBLs以CTX-M-14亚型为主。
CTX-M-14 ESBLs of Shigella strains was the predominant genotype in Tianjin .
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然而随着抗生素的广泛使用,临床频繁出现耐药志贺菌属菌株,而且呈现为多重耐药(multi-drugresistance,MDR)。
However , with the wide use of antibiotics , resistant isolates of Shigella spp. , especially multiresistant isolates with multi-drug resistance ( MDR ), occurred .