蛋白质的复性

包含体蛋白质的复性研究进展

Advance in vitro folding of inclusion body proteins

本文以溶菌酶为模型蛋白研究了蛋白质的复性机理。

In this paper , the mechanism of protein refolding is studied with egg white lysozyme as the model protein .

方法根据人工分子伴侣对重组蛋白质的复性机理，采用人工分子伴侣方法应用于重组内抑素的复性。

Methods Based on the reactive principle of molecular chaperone , we adopted an artificial molecular chaperone-assisted refolding method for recombinant endostatin refolding .

色谱复性使蛋白质的复性与纯化同步进行，简化了操作程序，提高了产品的回收率。

The means of chromatographic renaturation can renature and purify the protein at the same time , thus simplify the procedure of operation and promote the recovery ratio of the product .

重组包涵体蛋白质的折叠复性

Refolding of Recombinant Inclusion Body Proteins

蛋白质的体外复性是一个复杂的过程，需要精心设计。

The recovery of bioactive proteins from inclusion bodies is a complex process that should be carefully manipulated .

两类方法指导我们选择重组蛋白质的最佳复性条件，并给了我们许多有益的哲学思考。

At the same time , the research on protein refoking has gave us many enlightenments in the thoughts of philosophy .

蛋白质的变性、复性问题是与Hofmeister效应密切相关的生化基础课题。

The denaturation and renaturation of proteins is one of those basic problems .

大肠杆菌异源重组蛋白质的变性与复性

Denaturation and renaturation of alien recombinant proteins in E.coli

运用圆二色谱和荧光技术分析预测蛋白质二级结构，研究蛋白质的稳定性与蛋白质的变性复性动力学机制。

By the use of cirular dichroism and fluorescence techonologies to predict the secondary structure of protein , to determine the secondary structure of a protein , and to explore the unfold-refold mechanism of a protein .

色谱复性是一种非常有效的蛋白质复性方法，尺寸排阻色谱、离子交换色谱、亲和色谱已经成功应用于很多蛋白质的复性中。

Utilization of chromatographic columns for protein refolding is a good strategy . Size-exclusion chromatography , ion exchange chromatography and affinity chromatography have successfully refolded many proteins .