低速离心
- 网络low speed centrifugation
![低速离心](../cache/1176283.jpg)
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我们使用组织培养常用药品,根据细胞生长的不同状况,采取自然沉降或低速离心、部分消化与完全消化等方法,在消除成纤维细胞、纯化胎肝细胞中取得了较为满意的效果;
With the reagent most in use in thd tissue culture , the methods of natural precipitation , low speed centrifugation , partial dispersion and complete dispersion were used according to the different conditions of growth of the cells .
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本文采用一多功能流体动力学计算软件,详细计算分析了NASA低速离心压气机叶轮内部的三维速度场。
Based on a multifunctional CFD software , the three-dimensional velocity field within the impeller of NASA low-speed centrifugal compressor is computed in detail in this paper .
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DNA的收集采用低速离心法,避免了用钩子挑出对DNA造成的机械损伤。(5)用双蒸H2O溶解DNA,利于以后测序工作的进行。
Centrifugal method of low speed was used in the collection of DNA , comparing to hooking DNA , this method could avoide making machinery injury to DNA ( 5 ) Dissolved DNA with sterile distilled water which had advantage to sequencing .
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结论用羟乙基淀粉(HES)自然沉淀法和低速离心法分离脐血有核细胞,获得较满意的回收率;
Conclusions The recovery rate of neuclear cells of cord blood is gratifying using the isolation method of 6 % hetastarch ( HES ) sedimentation and low speed centrifuge .
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采用改良的蛋白酶-K法,在裂解细胞核之前加入不含CTAB的提取介质Ⅰ,经低速离心后可有效将多糖、多酚等物质与细胞核分离。
The improved CTAB-protease K method was suitable to remove polysaccharides and polyphenols by low-speed centrifugal because the solvents I , which did not contain CTAB , could not dissolve karyon but membrane .
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方法先用钙离子载体A23187诱导精子发生顶体反应,然后用水浴超声波和低速离心法分离得到纯牛精子头。
Methods Acrosome reacted bovine spermatozoa heads ( A 23187 induced ) were separated from their tails with ultrasonic treatment and centrifugation .
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理想的方法是用低速离心加培养基稀释法。
The ideal method is low speed centrifugalization and dilution medium .
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低速离心喷雾机结构的改造
Structure Improvement of the Low Speed Centrifugal Sprayer
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一种低速离心调速器的研究
Study on one kind of slow-speed centrifugal governor
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低速离心压气机非稳态流和机匣处理的数值分析
Numerical analysis for the unsteady flow and casing treatment in a low speed centrifugal compressor
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通过低速离心法分离未包载入红细胞的磁流体。
Through low-speed centrifugation separated from red blood cells did not pack the magnetic fluid loading .
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主要是经过两次低速离心,一次蔗糖梯度超速离心,即可得到一定纯度的细胞膜。
A fairly pure preparation can be obtained through two runs of low-speed centrifugation and a single run of high-speed centrifugation in sucrose gradient .
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巨细胞病毒感染的快速诊断&低速离心和单克隆抗体间接免疫荧光方法检测早期抗原
Rapid diagnosis of cytomegalovirus infection : detection of CMV using cell culture , low centrifugation and immunofluorescence with monoclonal antibody to early nuclear antigen
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低速离心分离脂质体与游离药物,高效液相色谱法测定阿糖胞苷的含量,并计算脂质体包封率。
Liposomes and free drug were separated with lower speed centrifugation . The content of cytarabine was determined by HPLC , and to calculate encapsulation efficiency .
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目的探讨减压抽滤、低速离心、高速离心、壳聚糖絮凝、活性炭脱色作为前处理方法对活血通络水提液膜过程中通量及膜污染度的影响。
The definition of membrane fouling was presented , the progress in membrane fouling research were thoroughly reviewed , and the technical measures for lessening membrane fouling effect .
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结果表明,经两次低速离心和两次高速离心,分离所得线粒体具有较高的呼吸活性和ADP/O比值,线粒体完整率达70%左右。
Results show that , under this experimental condition , isolated mitochondria have high respiratory activity and ratio of ADP / O. The intactness of isolated mitochondria is about 70 per cent .
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将消化的组织液液过200目滤网后,利用低速离心和反复沉降的方法,得到内膜腺上皮细胞经免疫组织化学鉴定,其纯度达95%以上。
The digestion was passed over the 200 sieve , purified the cells after gentle centrifugalization and natural sedimentation . Identified the cells we had by immunohistochemistry , the purity rate endometrial glands of is more than 95 % .
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使用高频动态压力传感器成功测量了实验用低速离心压缩机在小流量下详细的内部流动参数,对其旋转失速现象进行了分析。
The detailed flow parameters of the low-speed centrifugal compressor at small flow rates were successfully acquired using high-frequency dynamic pressure transducer . Based on the above experimental data , there is a stall cell rotating circumferential in the low-speed centrifugal compressor .