单链噬菌体

抗HCV解旋酶人源性单链噬菌体抗体的筛选

Screening of Human ScFv Antibody of HCV Helicase

SLE患者自身抗体单链噬菌体抗体库构建及抗体活性检测

Patients with SLE and Detection of Antibody Activities

结论成功地构建出了一个有效库容量为4.75×106的鼠抗LPS单链噬菌体抗体库。

Conclusion A 4.75 × 106 murine anti-LPS single chain phage antibody library was successfully constructed .

人源化抗血小板膜糖蛋白GPⅡb/Ⅲa单链噬菌体抗体的研究

Study on Humanized Anti-Platelet GP ⅱ B / ⅲ A Phage Single Chain FV Antibody

结果：从单链噬菌体抗体库中筛选出噬菌体抗体并进行富集，经鉴定为抗MAGE鄄A1的特异性噬菌体抗体。

Results : The screened phage antibodies were specific for MAGE-A1 . The soluble antibody was also specific for MAGE-A1 ;

EL-4荷瘤鼠单链噬菌体抗体库的构建

Construction of single-chain phage antibody library of cancer-bearing EL-4 mice

结论：成功构建了EL-4荷瘤鼠的单链噬菌体抗体库。

Effective content was 3.34 × 106.Conclusion : Single-chain phage antibody library of cancer-bearing EL-4 mice was successfully constructed .

目的：构建一个EL-4荷瘤鼠的单链噬菌体抗体库，为筛选高特异性和高亲和力的单链抗体做准备。

Objective : To construct a single-chain phage antibody library of cancer-bearing EL-4 mice , so as to screen single-chain antibody of high personality and high affinity .

抗脂多糖单链噬菌体抗体在大肠埃希菌中的表达及筛选

Expression and Screening of Anti-lipopolysaccharide Single-chain Phage Antibody in E.coli

鼠抗脂多糖单链噬菌体抗体库的构建

Construction of anti-lipopolysaccharide single-chain phage antibody library in mice

乙酰胆碱受体单链噬菌体抗体的筛选与鉴定

Selection and analysis of single chain antibodies against acetylcholine receptor from phage antibody library

结果获得了6×1010的大容量单链噬菌体抗体库。

Results A large phage antibody of 6 × 10 10 in size was constructed .

目的构建人源抗红细胞A抗原单链噬菌体抗体库。

Objective To construct a phage display library of human single-chain Fv antibodies against blood group A substance .

方法大鼠血管内皮细胞蛋白质免疫BALB/c小鼠，采用噬菌体抗体库技术构建小鼠抗大鼠血管内皮细胞的单链噬菌体抗体库。

Method BALB / c mice were immunized for 4 times with rat aortic endothelial cell . Then the phage display system was used to construct a single-chain Fv fragment ( ScFv ) cDNA library from the total RNA of immunized mice .

核酸分析试验显示，噬菌体XY-1为6000bp的单链RNA噬菌体。

Analysis test for Nucleic acid revealed that bacteriophage XY-1 was a 6000 bp , single-stranded RNA bacteriophage .

抗人肺癌单链抗体噬菌体库的构建及特异性单链抗体的鉴定

Construction of human phage display antibody ScFv library and identification of antibody ScFv against lung adenocarcinoma

目的构建抗P-选择素的全套单链抗体噬菌体表面展示文库，从中筛选与P-选择素高亲合力的单链抗体，为其临床应用奠定基础。

Objective Anti-activity platelet phage display library was constructed from mouse immunized with P-selectin , which has been used in clinical usage .

我实验室利用噬菌体抗体库技术，构建了全人源肝癌单链抗体噬菌体库，并筛选到了一株肝癌特异性单链抗体。

A single-chain antibody ( ScFv ) which is specific for hepatocellular cancer was selected and obtained from the human ScFv phage display library in our laboratory previously .

总之，通过对抗猪脂肪细胞膜蛋白单链抗体噬菌体文库的构建，我们对抗猪脂肪细胞膜蛋白单链抗体的研究进行了一定的摸索，这将为该抗体的进一步研究打下基础。

In a word , we take some method in researching single chain antibody by constructing scFv phage library against porcine adipocyte plasma membrane protein , which is very important to study immunization against adipocyte plasma membranes .

目的构建人源抗肝癌单链抗体基因噬菌体表面呈现文库。

Objective To construct the phage display library of anti-human hepatocellular carcinoma ScFv .

结论初步建立了抗甲状腺刺激性免疫球蛋白单链抗体的噬菌体抗体库。

Conclusion : The phage antibody library of ScFv against TSI was generated successful-ly .

目的：构建抗人卵巢癌全套单链抗体基因噬菌体表面呈现文库。

AIM : To construct the phage display library of anti human ovarian cancer single chain Fv antibody .

本文介绍了噬菌体展示技术的基本概况，着重阐述了噬菌体展示技术在鸡源性单链抗体、噬菌体随机肽库、人源化抗体以及过敏原的分析、拟糖多肽、蛋白质稳定性等方面的研究。

The article introduces the theory of phage display , stresses the application in chicken single chain antibody , humane antibody , phage peptide library , the anlysis of allergens , the research in glyco-replica peptide and stability of protein .

结论本研究采用RT－PCT法扩增出轻链及重链可变区基因，并进行了序列测定和分析，为进一步构建嵌合抗体以及单链抗体奠定了基础。乙酰胆碱受体单链噬菌体抗体的筛选与鉴定

The study laid a foundation of further development of chimeric and single chain antibodies . Selection and analysis of single chain antibodies against acetylcholine receptor from phage antibody library