单拷贝基因

Southern印迹杂交与水稻数据库搜寻的结果表明该基因在水稻基因组中是个单拷贝基因。

The results from both Southern blot analysis and database searching showed that it is a single copy gene in rice genome .

而且，已有的实验研究表明，该基因是一个单拷贝基因。

Moreover , Bmdsx is a single copy gene which have been experimented .

实验结果表明：使用己知DNA序列作引物设计板模，在同源序列碱基变异率不大于6.1%的条件下，通过设计和使用多对引物可保证能扩增出不同物种的同源单拷贝基因片段。

The results proved when base-different rate is below 6.1 % , using a few primers designed from the sequence of one species to amplify the homologous sequence of different species can ensure the success .

对随机挑选的452个BAC克隆的插入片段进行鉴定估计文库的平均插入为118kb，文库的基因组覆盖率为13.34倍，预计从文库中筛选到单拷贝基因的机率为99.984%。

Average insert size of the library is 118 kb evaluated from analysis of 452 BACs randomly picked from library . So the genome coverage of the library is 13.34 and the possibility to find a single-copy gene in the library is 99.984 % .

直接杂交法检测基因组的单拷贝基因

The detection of single copy genes in genome by direct hybridization technique

~（35）S标记探针检测人类单拷贝基因

Preparation of ~ ( 35 ) s-labaled probe for detecting singe copy genes in human genome

基因定位或利用已经定位的单拷贝基因设计染色体位点特异的分子标记；

Locate gene in the chromosome , or screen some kinds of individuals with sequence tagged sites markers ;

朊蛋白基因是单拷贝基因，高度保守，但在物种间可能存在易感性相关基因。

The prion-protein genes are single-copied and high conservative , but there might be related munity gene existing between animals .

同时，我们发现着丝粒附近区域的P/A基因比例最高，并且大部分是单拷贝基因。

Meanwhile , the regions near centromeres were found to contain the highest proportion of P / A genes , mainly consisting of singleton genes .

一株被兔抗虫卵可溶性抗原高免血清检获的基因克隆，其虫源基因片段经分子杂交证明系单拷贝基因。

The foreign schisto gene of this clone was proved to be a single copy gene which could be hybridized with fragments from the adult worm genomic DNA .

我们发现多拷贝基因比单拷贝基因具有较少的AS形式，而且基因AS形式的数量与基因家族的大小存在负相关性。

We observed that duplicate genes have fewer AS forms than that of single-copy genes , and that a negative correlation exists between the mean number of AS forms and the gene family size .

基因组印记是后代仅表达亲本单拷贝基因的现象，植物印记主要发生在胚乳，具有调控胚乳发育及影响种子大小的作用。

Genomic imprinting is a phenomenon that the gene is expressed primarily or exclusively from either the maternal or paternal allele and plant imprinting has been described primarily in endosperm . It plays a role in regulating endosperm development and affecting seed size .

用原位PCR方法检测培养细胞中的单拷贝SRY基因

Detection of SRY gene in cultured cells by in situ polymerase chain reaction

通过聚合酶链式反应（PCR）技术扩增得到X-Y染色体上的单拷贝同源基因片段（Amelogeningene）。

Single copy sequences of the X - Y homologous Amelogenin gene were amplified from the DNA extracts by PCR technique .

每个个体均不多于两种DRA序列，提示鲸类的DRA座位是单拷贝的基因。

No more than two sequences were found in each individual at the DRA locus , strongly suggesting only one DRA locus was amplified in this study .

方法采集以男性为供者的70例女性肾移植患者术后外周血白细胞，用套式PCR扩增单拷贝SRY基因来鉴定患者血中是否存在微嵌合体。

Methods Leukocytes were collected from peripheral blood of 70 female recipients having received kidneys from males for the identification of microchimerism by means of amplifying the single copied sex determine region Y gene ( SRY ) by nested PCR .

根据使用引物的不同，它可检测重复区段（着丝点、端粒或Alu家族）和单拷贝序列/基因。

PRINS can detect repetitive ( e.g.centromeric , telomeric or Alu repeats ) and single copy sequences / genes , depending on the primers used .

我们采用二轮PCR的方法对4个单拷贝的文昌鱼Pax基因进行筛选，共获得24个阳性克隆，平均每个基因对应6个克隆。

We further formulated the library into characteristic DNA pools for two-round PCR screening for 4 single copied Amphi-Pax genes and identified total of 26 positive clones with average of 6.5 clones for each gene .