印迹转移
- 网络blotting;Western Blotting
![印迹转移](../cache/1190191.jpg)
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SDS-PAGE和蛋白印迹转移受体配基结合实验证明重组菌株分泌表达了sIL-6R,72h的表达量达到最高。
SDS-PAGE and receptor-ligand binding assay show that sIL-6R was expressed by this strain and the maximum level is at 72h .
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对32例原发性食管癌组织DNA进行Southern印迹转移,发现有15例(47%)int-2基因扩增,扩增程度为2~8倍,其中10例淋巴结转移癌全部扩增。
Amplification of int-2 in 32 primary esophageal squamous cell cacinomas have been detected by Southern blot hybridization . The result showed amplification of int-2 gene in 15 cases ( 47 % ) . The amplification was from 2 to 8 fold .
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用蛋白质印迹转移技术分析正常及硒性白内障大鼠晶状体及房水中蛋白质的性质
Western Blot Analysis of Lens Proteins and Aqueous Humor in Normal and Cataractous Rat Eyes
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免疫印迹法证实转移SCC中basigin/CD147的表达增高。
Immunoblot analysis revealed that basigin / CD147 expression increased in metastatic SCC .
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应用Southern印迹杂交法检测转移基因在宿主细胞中的整合
Using Southern Blot Analysis to Determine the Integration of Transduced Genes in the Genomic DNA of Host Cells