碱基识别

在细胞内，siRNA通过碱基互补识别并结合相应的靶mRNA链，引导核酸酶对此mRNA链切割、消化，最终导致靶基因转录后沉默。

SiRNA can combine with the target mRNA chain by identifying bases complementary pairing , and guiding the Nucleic acid enzyme cut and digest the mRNA chain . Eventually it leads to target genes silence after transcription .

通过比较氢键占有率，我们发现非特异性结合体系的大部分氢键都属于蛋白质和磷酸根之间的氢键，这些氢键对于DNA碱基的直接识别贡献不大。

By comparing the hydrogen bond occupancy , we found that most hydrogen bonds for non-specific complex had a direct interaction with phosphate , and had little contribution to the direct base sequence recognition .

另外为了提高碱基错配识别的能力，在磁性纳米颗粒表面修饰了发夹探针。

Moreover , in order to enhance the discrimination capability for the single base mismatch , the magnetic beads were modified with the hairpin DNA .

钙调神经磷酸酶B亚基（PPP3R1）启动子区域新的5个碱基对缺失的识别及其与左心室肥厚的关系

Identification of a novel 5-base pair deletion in calcineurin B ( PPP3R1 ) promoter region and its association with left ventricular hypertrophy

为进一步揭示其分子识别机理和作用机制，我们探讨了DTIC对DNA分子单碱基错配的识别。

Finally , we have explored to utilize DTIC as a DNA molecular probe to sensitively detect specific DNA sequence and single-base mismatch or the single base mutations .