同尾酶
tónɡ wěi méi
- isocaudarner
同尾酶-
方法以牵引丝蛋白基因单体为基础，利用同尾酶聚合法构建含牵引丝蛋白基因单体六聚物的重组克隆质粒pUC18-6S和重组原核表达质粒pET-28a（+）-6S。
Methods Based on the monomer of the gene of spider dragline silk protein , the recombinant plasmids pUC-6S and pET-28a ( + ) - 6S were cloned by isocaudarner enzymatic polymerization .
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利用三引物PCR和同尾酶连接的策略实现多基因片段的重组
Multi-gene fragments recombination by TP-PCR and isocaudamer ligation
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本研究在反向PCR基础上，建立了一种新型染色体步移技术：同尾酶反向PCR。
A novel technique , isocaudarners inverse PCR ( II-PCR ), has been established for chromosome walking .