通用引物

通用引物PCR方法在地表水病原菌检测中的应用

Application of Pathogenic Bacteria Detection in Surface Water by Universal Primer PCR Method

通用引物PCR检测临床常见致病菌的实验研究

Universal Primer PCR for Detection of Clinic Bacteria

免疫捕捉-通用引物PCR检测食品中沙门氏菌

Immunocapture-Universal Primer PCR Detection of Salmonella sp. in Foods

选用6个叶绿体和4个线粒体植物细胞质通用引物，以所有材料基因组DNA进行筛选。

Genomic DNAs from all the samples were amplified with cytoplasm universal primers of 6 chloroplast and 4 mitochondrial .

一种新的基因芯片检测荧光标记技术：通用引物U2联合标记法

A new fluorescent labeling technique in microarray studies : universal primer U_2 labeling

建立了一种利用通用引物RT-PCR技术检测水中肠道病毒的方法。

The detection method for various enteroviruses in water sample by RTPCR with universal primers was established .

经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。

We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers , PCR , cloning and sequencing .

采用通用引物PCR配合SSCP和RFLP技术检测鱼病病原菌

Universal primer PCR with SSCP and RFLP for identification of fish disease pathogens

以标准NDV毒株RNA为模板，用通用引物进行荧光RT-PCR扩增，扩增产物的Tm值一般在（88±1）℃范围内；

The Tm values of the specific amplification products are always ( 88 + 1 ) C.

基于荧光通用引物的多重定量RT-PCR基因表达谱分析平台的构建、优化及其应用

Development , optimization and application of the expression analysis platform based on multiplex quantitative RT-PCR using fluorescent universal primers

检测了102例脑脊液标本，与培养法相比，通用引物PCR的敏感性为92.3%。

Compared with culture method , the sensitivity of the universal PCR for detection and identification of bacteria from 102 body fluids was 92.3 % .

根据Hoffmann等发表的通用引物序列扩增HA和NA基因并测序。

The HA and NA gene were amplified using the universal primer pairs according to Hoffmann .

方法用I类整合酶基因通用引物对临床分离大肠埃希菌进行PCR检测，并对阳性菌株进行接合转移实验。

Methods To detect the gene of class ⅰ integrase by PCR with universal primer and conjugal transfer the plasmid of class ⅰ integron positive strains .

鉴别鱼病病原菌的通用引物PCR-SSCP技术

Universal Primer PCR-SSCP for Identification of Fish Disease Pathogens

方法盐析法提取淋巴细胞白血病患者外周血白细胞DNA，TCR-γ基因重排通用引物和降落式PCR扩增基因重排。

Methods The DNA of peripheral blood leucocytes from lymphoid leukemia patients were extracted for amplification of the TCR - γ gene rearrangement with the consensus primers and touch-down PCR .

通用引物PCR扩增创伤感染细菌16S-23Srdna间区的研究

PCR Amplification of 16S-23S rDNA Intergenic Spacer Regions of Bacteria in Trauma Infection by Universal Primers

应用通用引物的RT-PCR技术检测韩国宫颈癌患者中MAGE和GAGE基因的表达

The expression of MAGE and GAGE genes in uterine cervical carcinoma of Korea by RT-PCR with common primers

方法：采用通用引物多聚酶链反应（PCR）及原位多聚酶链反应（PCR-ISH）对128例喉良恶性病变进行了检测。

Method : 128 paraffin embedded laryngeal squamous cell carcinoma and laryngeal epithelium hyperplastic lesions were detected by polymerase chain reaction ( PCR ) and PCR ISH for herpesviridae .

结论TCR-γ基因重排通用引物结合降落式PCR可有效扩增T淋巴细胞白血病基因重排，可用于T淋巴细胞白血病的辅助诊断。

Conclusion Consensus primers for studying TCR - γ gene rearrangement in combination with touch-down PCR can effectively amplify the clonal TCR - γ gene rearrangement in T lymphoid leukemia .

方法：应用虫媒病毒的通用引物逆转录-聚合酶链反应技术（RT-PCR）。

Methods : Serum samples from 168 patients with unknown fever and the samples mosquitoes were assayed by reverse transcription polymerase chain reaction ( RT-PCR ) .

该通用引物最低可检测8CFU／ml大肠埃希菌，250CFU／ml金黄色葡萄球菌。

The universal PCR could detect as few as 10CFU / ml E. coli or 250 CFU / ml S. aureus .

方法特异性测定：用皮肤病原真菌通用引物进行PCR，扩增大肠杆菌DNA、犬肝炎病毒DNA及小鼠肝癌H22细胞DNA。

Methods The determination of the specificity : E. coli DNA , Canine hepatitis virus DNA and mouse hepatoma carcinoma H_ ( 22 ) cell DNA was amplified by universal primers .

方法采用1对Cyt-b基因通用引物对人和19种动物共171例样本的mtDNA进行PCR扩增，琼脂糖凝胶检测扩增产物，ABI377测序仪及荧光测序技术分析扩增产物的DNA序列。

Methods mtDNA of 171 samples from human to 19 species of animals were tested by a pair of universal cyto b PCR primer .

提取浮游总菌的总DNA和RNA，利用通用引物扩增16Srdna，转化实验后挑选阳性克隆测序分析。

Then we employed universal primer to amplify 16S rDNA from total DNA and total RNA from planktonic bacteria , respectively . Positive clones were chosen for sequencing .

目的为探讨人乳头瘤病毒（HPV）与外阴白斑发生的关系，应用通用引物聚合酶链式反应和反向点探针杂交方法对58例外阴白斑进行HPV6,11,16,18,31,33等型别进行快速检测及基因分型。

AIM To establish the correlation between the infection of human papillomavirus and occurrence of vulvar leukoplakia . METHODS A reverse hybridization blot probe assay was used .

利用真菌固有的18Srdna保守区域设计通用引物，确立靶基因进行分析。

Designed universal primers by the public 18S rDNA conserved regions of the fungi , and established target genes for analysis .

设计并合成了一对聚合酶链反应（PCR）的通用引物，可以从小鼠杂交瘤的cDNA中扩增出免疫球蛋白（Ig）K链可变区（VK）基因。

We have designed two oligonucleotide primers to amplify the cDNA of mouse immunoglobulin K chain variable domain ( Vk ) by the polymerase chain reaction ( PCR ) .

目的报告一种新的基因芯片荧光标记技术：通用引物U2联合标记技术（universalprimerU2labeling，UPL）；

Objective To develop a new method for fluorescent labeling technique , universal primer U2 labeling ( UPL ), for microarray studies .

这些转基因小鼠的基因型鉴定均使用设计在Cre基因编码区的通用引物。

The genotyping of these transgenic mice were performed using a pair of common primers designed in the coding region of Cre gene .

应用通用引物和螺杆菌属特异引物对Hh的16SRRNA基因行扩增测序，并和近缘菌进行同源性比较，以明确其生物学地位。

The 16S rRNA gene of 4 strains of Hh were amplified , sequenced and analysed , using bacterial universal and Helicobacter genus specific primers .