低熔点琼脂糖

利用低熔点琼脂糖纯化DNA转化粗糙脉孢霉

Transformation of Neurospora crassa with low melting point agarose purified DNA

液化低熔点琼脂糖凝胶直接消化DNA法在构建突变体中的应用

Application of Making Mutants by the Method of Direct Digestion DNA in Liquified Low Melting Agarose Gel

以正常男性DNA作为模板，用本文设计的SRY基因的1对引物组合进行PCR法制备DIG探针，然后通过低熔点琼脂糖电泳回收将扩增产物纯化，得到SRY基因探针。

Complex genomic of human as template , using a pair combination of primers of SRY gene designed by us , we labeled the probe with DIG by PCR .

从低熔点琼脂糖凝胶中切下目的DNA，在68℃完全熔化后用5倍体积的双蒸水稀释，该化合物即可直接用于酶解反应。

The slice of interesting DNA was taken out from the low melting agarose gel and was completely melted at 68 ℃ . Then the melted slice was diluted with 5 volumes of distilled deionized water . The mixture could be then directly used for digesting DNA with appropriate enzyme .

该方法利用差速离心分离植物细胞核，经低熔点琼脂糖块或低熔点琼脂糖微珠包埋，蛋白酶K原位裂解后制备大分子量核DNA。

Generally it includes isolation of purified nuclei by differential centrifugation , embedment of the nuclei in either low-melting-point agarose plugs or microbeads , and digestion with proteinase K in situ to release high molecular weight ( HMW ) DNA .