表皮干细胞

分别取成模糖尿病和正常大鼠背部全层皮肤，采用胰蛋白酶和乙二胺四乙酸联合消化法分离表皮，Ⅳ型胶原黏附法纯化培养表皮干细胞。

Full-thickness skins of the back of diabetes mellitus group rats and normal rats are taken respectively . Enzyme digestion combined with type IV collagen attachment method is used to separate , culture and choose rat ESCs .

结果：①表皮干细胞的分离、扩增及鉴定：在扩增的细胞拟接种于无细胞真皮前，进行角蛋白19免疫组织化学鉴定、以流式细胞术进行α6、CD71鉴定。

RESULTS : ① Isolation , amplification and identification of epidermal stem cells : Before the amplified cells seeded on the acellular epidermis , keratin 19 ( K19 ) was identified with immunohistochemistry , and α 6 and CD71 with flow cytometry .

ES细胞源性表皮干细胞与胶原海绵构建组织工程皮肤

Reconstructed Tissue-engineered Skin with ES Cell-derived Epidermal Stem Cells and Collagen Sponge

RNA干扰抑制整合素β1在人表皮干细胞中的表达

Inhibition of integrin β _1 expression in human keratinocyte stem cells by vector-1 based interfering RNA strategy

目的研究129小鼠胚胎干细胞（EScell）源性表皮干细胞在同种小鼠全层皮肤缺损的生长和分化，探讨ES细胞源性表皮干细胞对全层皮肤缺损的修复作用。

Objective To provide a new way for treatment of full thickness skin defects by embryonic stem ( ES ) cell-derived epidermal-like stem cells .

P物质调控表皮干细胞β-catenin表达的机制及其在ESC分化中的作用研究

The Regulating Mechanism of SP on the Expression of β - catenin in Epidermal Stem Cells and Its Effects on the Differentiation of ESC

以ES细胞源性表皮干细胞为种子细胞构建皮肤类似物，探讨其在皮下的分化潜能。

To explore the differentiation potency of skin analog reconstructed with ES cell-derived epidermal stem cells and dermal analog in hypodermis .

表皮干细胞（epidermalstemcells，ESCs）是一类存在于皮肤组织中的多能性细胞，能够自我更新且具有多相分化潜能。

Epidermal stem cells ( ESCs ) are multipotent stem cells resided in the epidermis , which are self-renewal and potential to differentiate into different types of cells .

而A组实验创面的表皮干细胞数又较B组实验创面增多。结论经用荷负电气溶胶照射大鼠烧伤创面能促进表皮干细胞的增殖。

The experimental wounds in group A contained much more epidermal stem cells than in group B.Conclusion The application of the aerosol with negative electric charge can stimulate the proliferation of epidermal stem cell in the wounds .

综上所述，感觉神经肽SP可以诱导表皮干细胞进入分化状态，并在创伤愈合过程中诱导表皮干细胞发生定向迁移，这一研究丰富了创伤愈合中表皮干细胞的分子生物学调控理论

In summary , the neuropeptide SP induce ESC migration and come into differentiation state , this may be the molecular mechanisms for SP promoting wound he

2EGF是HaCaT细胞诱导MSCs向表皮干细胞分化的因素之一。

EGF is one of factors involved in differentiation of MSCs to epidermal stem cells .

近年来，表皮干细胞在组织工程、细胞治疗上都显示出巨大的应用潜力，其多能性也日益引起人们关注，尽管如此，其多能性和自我更新能力仍远弱于ESCs。

Recent years have seen huge application potential of epidermal stem cells in tissue engineering and cell therapy .

皮肤组织具有很强的再生能力，基底层中的表皮干细胞（keratinocytestemcells，KSC）在组织自我更新、损伤修复和肿瘤发生中起重要作用。

The keratinocyte stem cells ( KSC ) are located in epidermal basal layer and play a central role in tissue self-renewal , wound healing and neoplasm formation .

同时表达角蛋白K19、β1整合素认为是表皮干细胞。

Meanwhile , keratin K19 and β 1 integrin were taken as epidermal stem cells .

通过免疫组化方法，利用CK19作为特异标识分子，观察人表皮干细胞在不同解剖结构皮肤组织的定位。

By means of the monoclone antibody of cytokeratin 19 , to observe the localization of CK19-positive-cells in various anatomic sites of human skin .

在研究Wnt-1对人表皮干细胞的分化与增殖的影响时，我们借助了重组腺病毒基因导入技术将Wnt-1导入到人表皮干细胞内。

And then , using recombinant adenovirus gene introduction technology , we expressed Wnt-1 in epidermal stem cells .

[目的]作为毛囊外根鞘一部分的bulge区是近来公认的表皮干细胞池。

[ Purpose ] The bulge region , a portion of the outer root sheath , contains the hair follicle stem cells , which shows multipotency to differentiate into almost all epithelial cell types .

结论ES细胞源性表皮干细胞为种子细胞与类真皮构建的两种组织工程皮肤在体内具有修复缺损皮肤及分化为表皮及毛囊样、皮脂腺样和汗腺样等皮肤附属结构的潜能。

Conclusion The two types of tissue-engineered skin reconstructed with ES cell-derived epidermal stem cells and dermal analogs can restore the skin defects and have the potency to differentiate into sweat glands-like , sebaceous glands-like and hair follicles-like structures in vivo .

结果表明，DMEM／F12是一种适合表皮干细胞体外增殖培养的培养基，在此培养体系中细胞可传代至11代。

The results showed that DMEM / F12 was a good cell culture medium for the epidermal stem cells and the epidermal stem cells can survive to the11 subculture in vitro .

目的观察整合素β1对人表皮干细胞（KSC）增殖与分化的影响。

Objective To investigate the influence of integrin β _1 on the proliferation and differentia - tion of human keratinocyte stem cells ( KSCs ) .

人永生化角质形成细胞系（HaCaT）不仅具有与表皮干细胞相似的表型、增殖和分化特性，而且其遗传特性稳定、不具有成瘤性，可作为组织工程皮肤种子细胞的理想候选者。

Human immortal keratinocyte line ( HaCaT ) has genetically stable characteristics and high percentage of epidermal stem cell phenotypes without tumorigenicity .

目的观察不同频率的低强度脉冲电磁场（LIPEMF）对皮肤表皮干细胞（ESC）增殖作用的影响。

Objective To observe the effects of a low intensity pulsed electromagnetic field ( LIPEMF ) on the differentiation and regulation of skin epidermal stem cells ( ESC ) .

组织工程皮肤的建立结果：表皮干细胞种植于无细胞真皮上1d后，细胞便贴壁并开始生长增殖，8d左右透过间隙见无细胞真皮上表皮干细胞渐增殖融合成片；

Construction of tissue-engineered skin : Epidermal stem cells were soon adhered to the surface after seeded on acellular dermal matrix for 1 day . Eight days later , the cells on the surface of dermal matrix fused ;

收集创面组织标本，进行HE染色、β1整合素及角蛋白14（K14）免疫组化，观察皮肤表皮干细胞在创面愈合过程中的分布情况。

The wound specimen were collected and dyed with HE , and immunohistochemistry of integrin β 1 and keratin 14 ( K14 ), while the distribution of ESCs in wound was observed .

方法用Ⅳ型胶原筛选的方法培养并鉴定表皮干细胞，经体外与角膜缘基质细胞共培养诱导分化，免疫组化检测角膜上皮细胞特异标志物K12表达。

Methods Epidermal stem cells enriched by adhering to type ⅳ collage were induced in vitro by coculture with corneal stromal fibroblasts . The method of HCC were used to show the expression of K12 which is expressed specially in corneal epithelial cells .

目的构建针对整合索（intergrin）β1特异性的siRNA表达载体，并转染人表皮干细胞（KSC），探讨整合素β1对KSC增殖与分化的影响。

Objective To construct small interfering RNA ( siRNA ) expression vectors specific to integrin β 1 and study the role of integrin β 1 in regulating human keratinocyte stem cell ( KSC ) prolifer - ation and differentiation in vitro .

端粒酶在体外培养人表皮干细胞中的表达

Expression of telomerase in human epidermal stem cell cultured in vitro

应用原瓶黏附分选表皮干细胞的方法学特点

Methodological characteristics of former plate attachment to isolate epidermal stem cells

去分化来源表皮干细胞的分离与鉴定

Isolation , cultivation and identification of dedifferentiation derived epithelial stem cells

体外分离、纯化人表皮干细胞促进皮肤的功能生理性修复

Separation culture and identification of human epidermal stem cell in vitro