质粒转化

甘草Ri质粒转化及不同理化因子对甘草毛状根生长的影响

Ri plasmid transformation of Glycyrrhiza uralensis and effects of some factors on growth of hairy roots

实验结果还表明，REMI的转化效率是一般质粒转化效率的19.12～22.72倍。

REMI transformation efficiency was 19.12 ~ 22.72 times higher than normal plasmid transformation without restriction enzyme .

对酵母体内一对一验证的阳性克隆进行DNA测序。筛选的阳性重组表达质粒转化表达菌E。

Sequence the verified positive clones . The positive recombinant plasmids were made sequence .

PCR扩增同源重组片段筛选转基因胚胎应用细菌质粒转化技术，将大肠杆菌K（？）

Identification of Transgenic Embryo Using Homologous Recombinant Fragments The recombinant plasmid of E.

Ti质粒转化胡萝卜细胞的初步研究

Preliminary study of transformation of Ti plasmid in carrot cell

但southern杂交表明，这两个菌株并不是由于质粒转化而获得苯菌灵抗性。

However , southern analysis showed that the resistance of the transformants were not to linked to the vector plasmid .

栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长，纸电泳检测结果表明其合成了冠瘿碱，表明Ti质粒转化成功。

The detection of nopaline in the crown galls showed that T_DNA of Ti plasmid had been transformed successfully .

发根农杆菌Ri质粒转化康乃馨初步研究

A PRELIMINARY STUDY ON CARNATION BY RI PLASMID OF Agrobacterium rhizogenes

Ri质粒转化烟草影响因素的研究

Study on factors influencing the efficiency of Ri plasmid transforming of tobacco

Ri质粒转化番茄的初步研究

Using Ri-plasmid to Transfer Tomato Primarily

Ri质粒转化烟草的影响因素及豌豆凝集素基因在烟草发根中的表达

Factors influencing the transformation of Ri plasmid to tobacco and expression of pea lectin gene in tobacco hairy roots

构建的重组表达质粒转化至大肠杆菌BL21（DE3），用IPTG诱导表达；

Coli BL 21 ( DE3 ), and expressed by IPTG induction .

将重组质粒转化于E.ColiBL21（DE3）中进行诱导表达。

Then these recombinant expression plasmids were transformed with E. coli BL21 ( DE3 ) .

对Ri质粒转化烟草的影响因素进行研究，以期获得较为理想的实验系统。

Factors influencing the transformation of Ri plasmid to tobacco were studied in this paper in order to get the ideal experimental system .

然后将重组质粒转化到大肠杆菌BL21（DE3）株中并用IPTG于37℃诱导培养获得表达，表达形式为包涵体。

Coli BL21 ( DE3 ) strain , and then induced by IPTG at 37 ℃ .

稀土元素铕对掌叶大黄Ri质粒转化根生长及蒽醌类成分的影响

Effect of Eu ~ ( 3 + ) on Growth and Production of Anthraquinone in Ri Plasmid-Transformed Root and Normal Roots of Rheum palmatum

然后将重组阳性质粒转化大肠杆菌BL21（DE3）并用IPTG于37℃诱导表达。

Then the recombinant plasmid was transformed into E.coli BL21 ( DE3 ) and induced with IPTG .

以此重组质粒转化大肠杆菌DH5α，在IPTG诱导下表达。

The recombinant plasmid was transformed into E. coli DH5 α, and expressed under IPTG induction .

将构建成功的FR9F-ACP4重组质粒转化到大肠杆菌BL21（DE3）中，并优化其最佳的可溶性表达条件。

The recombinant plasmid was expressed in E.coli BL21 ( DE3 ), the conditions of the expression were optimized .

重组质粒转化大肠杆菌BL21（DE3）菌株进行目的基因的诱导表达。

These recombinant plasmids were induced in Escherichia coli ( E.coli ) strain BL21 ( DE3 ) after transformation .

方法以CMV/LacZ质粒转化COS7细胞系，48小时后用XGal染色。

Method CMV / LacZ was transfected into COS 7 cell line by electroporation and X gal staining was Performed 48 hours later .

构建成功的重组质粒转化大肠杆菌BL21（DE3）pLysE细胞，经12%SDSpage鉴定融合表达蛋白。

Transform the constructed plasmid into E. coli BL21 ( DE3 ) pLysE cells and identify the expressed fusion protein by 12 % SDS PAGE .

同时，单菌落PCR法也可应用于重组质粒转化后的农杆菌的筛选，单菌落PCR法的扩增结果和农杆菌液扩增的结果一致。

The selecting of agrobacterium transformed with recombination plasmid could also use this method of PCR screening of individual bacterial colonies . The result of individual bacterial colonies PCR was as well as that of PCR using bacterial solution as template .

利用发根农杆菌Ri质粒转化植物材料是促进植物扦插生根的有效方法，但是这些转基因植物同时也产生了比较严重的表型变异。

More researches on improving rooting ability by the Ri plasmid of Agrobacterium rhizogenes have been taken at present . But the trans-genetic plants demonstrate serious phenotype variation at the same time .

将此重组质粒转化到大肠杆菌菌株DH5α中，重组菌株经IPTG诱导后，在乙醛指示平板检测到乙醇脱氢酶活性。

Coli DH5 α . The recombinant strains were induced by IPTG to express ADH ⅱ . The activity was determined by aldehyde indicator plates .

在此基础上，通过去除E2基因跨膜区序列，构建得到pET28a-BE2m和pBV220-BE2m，将这两个质粒转化大肠杆菌并诱导表达结果显示重组菌表达的目的蛋白分别占菌体总蛋白为35.7%和8.96%。

However , when the transmembrane sequence of E2 gene was deleted from the two plasmids the expression yield of the E2 was increased to 35.7 % and 8.96 % of the total bacterial protein , respectively .

再用NDM-1重组质粒转化如大肠杆菌中，用该含有NDM-1重组质粒的大肠杆菌左右筛选具有超级细菌抗性的体外模型对中药黄连进行了评价。

The NDM-1 recombinant was transformed into E coli which used to evaluate the anti-super-bacteria activity of Chinese Medicine Coptis chinensis Franch .

Ri质粒转化技术是植物基因工程的一个重要方面，它与植物化学研究手段的结合，为天然产物的持续开发提供了新的技术路线。应用细菌质粒转化技术，将大肠杆菌K（？）

The technique of Ri plasmid transformation is an important research area of plant genetic engineering . At present , Ri plasmid transformation has been combined with phytochemistry , The integration of two fields has provided newfashioned technique for exploitation of natural products .

将重组质粒转化到大肠杆菌BL21（DE3）中进行表达，表达的核蛋白再经Ni2+-NTA亲和层析纯化。

Then they were transformed into Escherichia coli BL21 ( DE3 ) competent cells for expression . The expressed nucleoprotein was purified using Ni2 + - NTA Column under denaturing conditions .

将该重组质粒转化大肠杆菌BL21（DE3）感受态细胞，经诱导获得大小约173kDa的表达蛋白（rPMT）。

Coli BL21 ( DE3 ) competent cells . After induction by IPTG , a 173 kDa recombinant protein ( named rPMT ) was expressed .