脂质体介导法

用脂质体介导法将pRevTRE/HSVtk/teton导入乳腺癌细胞株（MCF7）。

Transfection of pRevTRE / HSVtk / Tet-On into MCF-7 cells was performed by using lipofectin .

采用脂质体介导法将构建的重组质粒pVVP3IL-18HN体外转染Hep-2细胞，运用MTT法检测不同质粒浓度、不同作用时间，该重组质粒对喉癌细胞Hep-2的杀伤率。

The recombinant plasmid was introduced into Hep-2 cells by liposome , then suppression rate of Hep-2 of different time and different quantity was calculated according to MTT results .

用含有多瘤病毒mT基因的重组质粒Zip－mT以脂质体介导法转染初代培养的MTSC，经过800μg／ml的G418筛选，得到5个MTSC克隆。

Primary MTSC cultures were transfected with Zip mT ( harbouring polyoma virus middle T gene ) together with lipofectin . After selection by G418 up to 800 μ g / ml , 5 MTSC clones have been obtained .

脂质体介导法将其转染COS7细胞，72h以RTPCR检测转染细胞中外源hFL基因的转录、ELISA法及脐血CD34+细胞增殖实验测定转染细胞上清中hFL的含量和活性。

The transcription and expression of hFL in the transfected COS 7 cells were assayed by RT PCR , ELISA and the experiment of the human umbilical blood CD34 + cell multiplication , respectively , at 72 hours after transfection .

脂质体介导法转绿色荧光蛋白基因鸡的技术路线研究

The Study of Technology Route of Transgenic Chicken Producing with Liposome-mediated Green Fluorescent Protein Gene

脂质体介导转染法原理及其研究近展

Mechanism of Transfection Mediated by Liposome and Its Research Advance