转染率

此外，PEI也被认为具有高转染率和使内涵体破裂的能力。

Additionally , PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity .

超声介导微泡造影剂对细胞和微血管以及基因在HUVEC内转染率的影响的研究

Study on Effects of Ultrasound-Mediated Microbubbles Contrast Agent on Cells , Capillaries and Transfection Efficiency of Gene in HUVEC

脂质体介导外源性DNA阳性转染率可高达30%～40%以上。

Transfected rate of exogenous DNA mediated by lipofectin was above 30 % ~ 40 % .

以NS组为对照并以GFP为荧光标记物，用流式细胞仪（flowCytometry，FCM）分析各实验组肝细胞的转染率。

TE of rat hepatocytes were analyzed by flow cytometry ( FCM ) using GFP as fluorescent marker .

【结论】共聚物的结构组成对DNA复合物的理化性质、毒性和转染率都产生较大的影响。

[ Conclusion ] The copolymer block structure strongly influences not only the physicochemical properties of the DNA complexes , but also their cytotoxicity and transfection efficiency .

TK的转染率可以通过应用改善载体来提高，如增殖型腺病毒。

The rate of TK transfection could be boosted by using proliferous adenovirus .

外源TFPI基因的转染率为23%。

The transfection rate of exogenous TFPI gene is 23 % .

结果经统计学分析显示，不同血清型AAV对三种细胞系转染率有显著差异。

RESULTS The transduction efficiency of AAV is distinct in the three lines .

质粒带有eGFP，荧光显微镜观察和流式细胞仪计数确定转染率。

Because of the plasmid with eGFP , fluorescence microscopy and flow cytometry were used to determine transfection efficiency .

结果：随着腺病毒感染复数量（multiplicityofinfection，MOI）增加，对Hep-2细胞转染率亦增加，100%转染所需的MOI为200。

Results : The more the multiplicity of infection ( MOI ), the more the adenovirus transfected efficiency to Hep 2 cells .

目前有研究发现，超声微泡在超声作用下可明显提高基因的转染率，本研究利用超声微泡技术联合超声辐照，观察TFO对TF的作用效率。

Present study has indicated that ultrasonic microbubble under the action of ultrasound irradiation can improve gene transfection efficiency .

结果：逆相蒸发法制备的脂质体包封率高于冻融&超声法（P＜0.01）。流式结果显示两种方法制备的脂质体转染率无显著性差异（P＞0.05）。

Result : The rate of liposome envelopment completed with the contra-phas-evaporated method is substantially ( P < 0.01 ) higher than the liposome completed with the thaw-freeze-ultrasonic method while the effect of transfection was of no sharp difference ( P > 0.05 ) .

HSV-TK／GCV系统抗肿瘤效果不佳，或对某些肿瘤细胞无效的原因可能是TK转染率低和／或旁观者效应低。

The inefficacy of HSV-TK / GCV system against tumors would result in low TK gene transfection rate or / and bad BSE .

FAM检测CEA转染率、成熟DC表型、CTL亚群比例、CTL体外杀伤率。

Detecting CEA transfection rate , the mature DC phenotype , the proportion of CTL subgroup , the killing rate of CTL in vitro with FCM . 3 .

与缺乏FOL受体的A549细胞相比，由此而来的合成物在过量表达FOL受体的KB细胞中有更高的转染率。

The formulated complexes exhibited a considerably higher transfection efficiency against FOL receptor over-expressing KB cells than FOL receptor deficient A549 cells .

脂质体介导的端粒酶反义寡核苷酸在人膀胱癌EJ细胞中的转染率及稳定性

In vitro transfection efficiency of telomerase antisense oligodeoxynucleotide mediated by liposome in human bladder cancer cell line EJ and its stability

通过检测报告基因β－半乳糖苷酶（β－gal）的表达，发现最高转染率只在一较窄范围内获得。

A narrow range of conditions was founded to produce maximal level of transgene expression , as detected using the reporter molecule β - galactosidase (β - gal ) .

用HEPES缓冲液能显著提高磷酸钙法转染率；

The transfection efficiency of calcium phosphate was greatly improved by using HEPES buffer ;

若能降低此载体的LCST，使其在体温附近发生相转变，将有望通过调解温度进一步提高转染率。

It is expected that the transfection efficiency of PNI-AAM-G 1-3 could be further improved by changing its LCST below the body temperature .

结论LPD是一种制备工艺简单、体外稳定性好、转染率高，具有应用潜力的非病毒载体系统。

Conclusion LPD could be prepared easily with small particle sizes and high transfection activities . LPD may be good non-viral vectors for applications in gene delivery .

在本研究中得出：电压在350～450V之间，电穿孔法转染率与电压呈线性关系；

We found that the transfection efficiency of electroporation was linear with the voltage which was set from 350 to 450V ;

48～72h阳性细胞明显增多，强度增强，高倍视野下转染率达20%～30%，多为明亮的绿色荧光的细胞。

From hour 48 to 72 the number of positive cells significantly increased , with strong intensity . The transfection efficiency was 20 % - 30 % under high-power field , and most of the cells were with green fluorescence .

人牙髓细胞受Ad-hBMP-7感染48h后即可检测到外源蛋白表达；当感染复数为75时，重组腺病毒对牙髓细胞的转染率达（91.1±1.0）%；

Higher transfection efficiency ( 91.1 ± 1.0 ) % could be obtained at MOI of 75.Western blot from dental pulp cells infected with Ad-hBMP-7 for 48h detected protein expression of a hBMP-7 gene .

以绿色荧光蛋白基因为报告基因，梭华Sofast转染率最高，约60%左右，而且在单个细胞中表达的GFP量多；

Using GFP as report gene , we found that the highest transfection efficiency can be acquired from Sofast among the four transfection reagents , it is about 60 % , moreover GFP expressed largely in single cell ;

基因载体必须具备安全、稳定、转染率高等特点。

The carrier must be safe , stable , efficient .

阳离子脂质体-DNA复合物与脂质-鱼精蛋白-DNA复合物体外细胞转染率比较

Transfection Efficiency Comparison of Cationic Liposome-DNA Complexes and Lipid-Protamine-DNA Complexes In Vitro

并进行转染率的计算，探索最佳转染浓度及时间。

And calculate carrying rate to explore the best carrying concentration and time .

最适感染复数为100，转染率可达90%以上。

When optimal MOI is 100 , transfection efficiency is above 90 % .

超声造影剂介导下增强腺病毒相关病毒心肌靶向转染率的实验研究

The Study of Enhancement of Recombinant Adeno-associated Virus Transgene to Myocardium by Targeted Echo-contrast Agent

但存在如何提高转染率，使细胞高效表达及生物安全性问题。

The problem lies in how to improve the transfection rate and target gene high expression .