菊粉

目的研究菊粉硫酸酯（inulinsulfate，IS）的制备及其抑制真菌活性。

Purpose To study the preparation and anti-fungus activity of inulin sulfate .

菊粉（Inulin）是一种新兴的脂肪替代物，不仅有利于人体健康，还能起到改善干酪质构的作用。

And inulin ( Inulin ), as a new fat substitute , is not only beneficial to human health , but also play a role in improving the texture of cheese .

在上述优化培养条件下，宇佐美曲霉产菊粉酶的酶活为11.02U／mL。

The enzyme activity was 11.02U/mL in the optimized condition .

黑曲霉Uγ-2菊粉酶发酵条件的研究

Studies on the Inulinase Fermentation Conditions of the Aspergillus Niger U γ - 2

黑曲霉Uγ-2（AspergillusnigerUγ-2）产菊粉酶的研究

Studies on production of inulinase from Aspergillus niger U_ γ - 2

果胶酶高产菌株Aspergillusniger3502的选育高产菊粉酶的黑曲霉菌株产酶酶系分析与研究

BREEDING OF HIGH YIELD STRAIN ASPERGILLUS NIGER 3502 FOR PECTINASE The studies on enzyme system of Aspergillus niger strains with high inulinase activity

酵母菌C10产菊粉酶酶学性质的研究

Study on the enzymatic properties of inulinase produced by yeast C10

研究了克鲁维酵母菊粉酶的酶学性质：最适pH值为5.0，在pH4.6～5.2稳定性较好；

The enzymatic properties of Kluyveromyces was studied : the optimum pH was 5.0 and stable in pH4.6 ~ 5.2 ;

采用菊粉酶活力测定的方法研究了酵母菌C10所产菊粉酶的酶学性质。

The enzymatic properties of inulinase produced by yeast C10 were investigated with assays of inulinase activity .

黑曲霉A24产酶代谢调节研究表明，菊粉酶属诱导酶，菊粉是黑曲霉A24合成菊粉酶的良好诱导物。

Regulation on Inulinase metabolism in Aspergillus niger A24 showed that inulinase was an inducible enzyme , with Inulin being the inducing factor .

克鲁维酵母Y-85菊粉酶的纯化和性质

Purification and properties of Inulinase from Kluyveromyces sp. y-85

对菊粉酶产生菌B-3-3发酵条件、酶的分布及酶学特性的研究

Study on Producing Inulinase Condition Contribution and Characteristics of Inulinase by Strain B-3-3

本研究以菊粉、甲基丙烯酸缩水甘油酯（GMA）、四甲基乙二胺（TMEDA）和活性染料为主要原料，通过接枝聚合制备菊粉凝胶，再经染色，制成不溶性呈色菊粉。

Insoluble chromogenic inulin gel was made through graft polymerization and staining with inulin , Glycidyl methacrylate ( GMA ) , tetramethylethylenediamine ( TMEDA ) and active dye .

首次利用固体发酵对筛选得到的一株克鲁维酵母S120高产菊粉酶的发酵工艺进行了初步研究。

This paper firstly studied the high levels of inulinase by Kluyveromyces S120 under solid-state fermentation ( SSF ) .

根据传统的鉴定方法及分子生物学鉴定，G7a最终被鉴定为Cryptococcusaureus（金黄色隐球酵母），这是关于海洋隐球酵母产菊粉酶的首次报道。

This marine yeast strain was identified to be a strain of Cryptococcus aureus according to the results of routine yeast identification and molecular methods . To our knowledge , this is the first report that Cryptococcus aureus from the marine environment could produce extracellular inulinase .

确定菊粉酸水解的最优条件为：菊粉浓度为20%、时间90min、温度75℃、硫酸浓度5%。

The best conditions were : the concentration of inulin was 20 % , time was 90 min , temperature was 75 ℃, the concentration of sulfuric acid was 5.0 % .

用15L自控发酵罐进行产酶条件控制试验，并在1000L罐上进行5批次酶发酵中试，平均菊粉酶活性达68.9u／ml。

The experiments of condition control for the enzyme production was carried out in a 15L fermenter . A large scale fermentation ( 1000L fermenter ) was performed five batches , and average inulinase activity of 68.9 u / ml achieved .

菊粉氢呼气试验精确反映口盲传输时间。

The inulin hydrogen breath test accurately reflects orocaecal transit time .

响应面方法优化菊粉酶液体发酵培养基的研究

Optimization of Submerged Fermentation Media of Inulinase with Response Surface Methodology

克鲁维酵母菊粉酶基因在毕赤酵母中的表达

Expression of the Inulinase gene from Kluyveromyces marxianus in Pichia pastoris

菊粉对猪肉脂肪组织中3-甲基吲哚含量的影响

The Effect of Inulin on 3-methylindole in Adipose Tissue of Pigs

菊粉酶高活力菌株的筛选及其产酶研究

Screening of high inulinase active strains and their enzyme yield conditions

1.5%菊芋干粉为合成菊粉酶较好的诱导物；

The better inducer was chicory powder with concentration at 1.5 % .

菊粉化学和微生物菊粉内切酶研究进展

A Progress of Studies on Inulin Chemistry and Microorganism Endo-inulinase

在酵母所产的菊粉酶中该酶活力已经达到了相当高的水平。

This is very high level of inulinase activity produced by yeasts .

在这些酶类中，菊粉酶受到了广泛的重视。

Among the enzymes , inulinase has received much attention .

菊粉酶的固态发酵及其保护剂的研究

The Study of Solid-state Fermentation for Inulinase Production and Its Protective Agent

菊粉酶活性平行地随菌体生长而增加。

The inulinase activity increased paralleled by the mycelium growth .

种子液制备方法对乙醇发酵影响显著，种子培养基中添加2%的菊粉有利于Y05诱导产酶。

Method for seed preparation had significant influence on the ethanol fermentation .

棉织物固定化菊粉酶酶解菊粉的研究

The Hydrolysis of Inulin with Immobilized Inulinase on Cotton Cloth