考马斯亮蓝

观察CTGFAS对ANGII诱导的细胞内总蛋白含量（考马斯亮蓝蛋白定量技术）、细胞直径大小（扫描电镜技术）、细胞内部超微结构（透射电镜技术）的影响；

The effect of CTGF AS on Ang II induced total cellular protein content ( Coomassie brilliant blue G250 method )、 change of cellular size ( determined by scanning electronic microscope , SEM ) and cellular ultrastructure ( observed by transmissive electronic microscope , TEM ) were observed respectively .

用奥林帕斯倒置相差显微镜及JVC摄像系统观察、拍摄与记录细胞生长情况，并于培养11d，用考马斯亮蓝和苏木精-伊红染色，证实相差显微镜观察的结果。

Olympus inverted phase contrast microscope and JVC video system were adopted to observe , shoot and record the cell growth , and Coomassie brilliant blue as well as HE staining were conducted at 11 days after culture to identify the observation under the contrast phase microscope .

伊红-苯胺黑染色、低渗肿胀实验和考马斯亮蓝染色法检测结果高度相关（r≥0.9981）。

The results from eosin-nigrosine staining , Hypoosmotic Swelling Test and Coomassie blue staining were highly correlated ( r ≥ 0.9981 ) .

通过扫描电镜、MTT测试法、考马斯亮蓝和碱性磷酸酶活性检测法，观察去抗原异种松质骨材料对细胞生长、增殖及功能表达的影响。

SEM , MTT assay and alkaline phosphatase ( ALP ) activity were carried out to observe the effect of AEXCB on cell growth , proliferation and functional expression .

方法：将SDSpage中的蛋白分别用银染、铜染和考马斯亮蓝染色，并对铜染或考马斯亮蓝染色后的蛋白进行相应的复染。

Methods : The proteins in SDS PAGE were stained with coomassie brilliant blue staining , copper staining and silver staining . Then the proteins were restained after coomassie brilliant blue staining or copper staining .

蒽酮反应、考马斯亮蓝、紫外扫描等测定结果表明，絮凝剂CBF的主要成分为多糖类物质。

Compound bioflocculant ( CBF ) is mainly composed of polysaccharide , which is determined by Anthrone reaction , Coomassie blue reaction and ultraviolet spectroscope method .

方法采用改良Lowry法和考马斯亮蓝染料结合法（Bradford法）测定蚯蚓提取物蛋白质含量；再使用两法测定标准牛血清白蛋白（BSA）水溶液在碱性蛋白酶水解前与后的蛋白质含量。

Methods Protein contents in earthworm extract and in bovine serum albumin ( BSA ) solution before and after enzymolysis were determined using Lowry 's modified assay and Bradford 's dye-binding assay respectively for comparing the difference between both .

分别对其施加10%0.5Hz的周期性应变，采用激光共聚焦显微镜技术和考马斯亮蓝染色方法对小鼠骨髓间充质干细胞系（D1细胞）细胞骨架进行形态观察、细胞F-肌动蛋白表达定量分析。

10 % 0.5 Hz cyclic strain force was applied on the mouse marrow mesenchymal stem cells at a different time , the cell was marked by doubling label technique using FITC-phalloidin and PI and Morphological was observed by confocal laser scanning microscope and BB staining .

利用NF-SH、Desal-DK和Les-90膜研究了操作条件对3种纯有机溶剂膜通量以及考马斯亮蓝甲醇溶液截留率的影响。结果表明，当透过溶剂为甲醇时，膜的压实效应比水明显；

Secondly , by observing influences of pressure on transport flux of solvents permeating through nanofiltration membranes ( NF-SH , Desal-DK , Les-90 ) and rejections in methanol solution with Brilliant Blue-G , studies showed that membrane compacting effect in methanol are more than water ;

染色：行银染和考马斯亮蓝染色；

Staining : with silver and coomassie brilliant blue staining .

考马斯亮蓝微板法测定细胞内蛋白总量。

Coomassie Brilliant Blue method was used to quantify the proteins in cells .

考马斯亮蓝检测三组蛋白的含量。

Three kinds of tissues protein content were determined using coomassie brilliant blue assay reagent .

用考马斯亮蓝测定植物粗提液中可溶性蛋白质含量方法的研究

Method research of measuring soluble protein contents of plant rough extraction using Coomassie Brilliant Blue

为了观察肌细胞骨架，对传统考马斯亮蓝染色法进行改良，并与免疫荧光染色法进行了比较。

The paraformaldehyde prefixed-coomassie brilliant blue staining is a convenient cytoskeleton staining method for muscle cell .

研究了考马斯亮蓝试剂和福林-酚试剂与乳粉蛋白质含量之间的显色关系；

Secondly , color reaction between protein content of milk powder and G-250 and Folin-Phenol reagent were established .

派给迪肯·尤弗雷茨的角色是在队里打头阵，银行一开门第一个进去开立帐户。（4）染色：行银染和考马斯亮蓝染色；

Deacon Euphrates had been assigned to be early in the line and the first to open an account . ( 4 ) Staining : with silver and coomassie brilliant blue staining .

结果：聚丙烯酰胺凝胶电泳考马斯亮蓝染色法结果显示，阴、阳离子交换示差组合色谱法，具有较好的分离纯化效果。

Results It was showed by PAGE that the differential combination ( chromatography ) of anion and cation exchange had much better purifying ability than anion and cation exchange ( chromatography ) of isocratic elution .

另外，实验还对纯溶剂与考马斯亮蓝溶液膜通量进行比较，发现在考马斯亮蓝的甲醇和乙醇溶液中，由于反射系数较小，通量较纯甲醇和乙醇溶剂的膜通量变化小。

Additional experiment studies the flux of some pure organic solvents and their organic solutions , results showed that due to reflect coefficient of organic solvent is far lesser than aqueous systems , when organic solvents are added with the solute , their fluxes are not changed greatly .