组成酶
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方法基于脱氢酶辅酶体系的原理,用丙酮酸钠作乳酸脱氢酶(LDH)的阻止剂,在TrisHCl缓冲溶液中,由NAD+-NBT-Brij98组成酶偶联放大系统。
Methods Based on the principle of dehydrogenase coenzyme system , sodium pyruvate was used as a lactate dehydrogenase ( LDH ) blocker , NAD + - NBT-Brij 98 was composed to enzyme coupling amplification technique in Tris HCl buffer .
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结论以PBST为缓冲液,以BSA和Casein无关蛋白作稳定剂组成酶结合物稀释液适用于HCV抗体酶联免疫诊断试剂的生产。
Conclusions The results indicates that enzyme combination diluent is suited for the production of HCV antibody enzyme linked immunosorbent diagnostic reagents , if the enzyme combination diluent regards PBST as buffer liquid and BSA and Casein irrelated albumen as stability .
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在DNS32菌株体内,这三种降解基因所编码的酶可能是稳定表达的组成酶。
The atrazine-degrading enzyme coded by the three degrading genes may be constitutively .
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SOD复合酶是由超氧化物歧化酶、过氧化氢酶、过氧化物酶组成的酶集团,能有效清除人体细胞中的自由基。
SOD complex enzyme is the enzyme group consisted of erythrocuprein , catalase , and peroxidase , which can effectively eliminate the free radicals in cells of the human body .
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然而,仅仅通过分步透析除去变性剂,Rubisco的亚基仍然不能重组成全酶,除非再加入分子陪伴蛋白60(cpn60)。
However , fully denatured Rubisco subunits still could not be reassembled into holoenzyme after gradual dialysis unless chaperonin 60 ( cpn60 ) was added .
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利用生物信息学软件对TRS-A及TRS-B编码的氨基酸序列进行了氨基酸组成、酶切位点、二级结构、三级结构等性质进行了分析预测。
The amino acid composition , cleavage sites , secondary structure and tertiary structure of TRS-A and TRS-B were analyzed and predicted by bio-information software .
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研究了聚合时间、单体组成对酶促共聚的影响。
Effects of polymerization time and composition of monomers were investigated .
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在细胞衰亡期细胞破碎其细胞内部的组成型酶释放到发酵液中,导致发酵液中酶活升高。
Released consisted-enzyme during the cell death period leads the increase of the xylanase activity .
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它们有同样的分子量但结构类型和特性却大不相同。这归因于组成同功酶的亚单位及结构不同。
The isomeric forms all have the same molecular weight but differing structural configurations and properties , usually due to different combinations or their component subunits .
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凝乳酶是酸性蛋白酶,它是组成皱胃酶的一种主要成分,在反刍动物的第四胃产生凝乳酶原,主要是水解Κ-酪蛋白的Phe105-Met106键。
Chymosin , the major component of rennet , is an acid protease produced in the fourth stomach of ruminants in the form of an inactive precursor prochymosin . It is responsible for hydrolysis peptide bond between Phe 105 and Met 106 in the Κ - casein .
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端粒酶是一种特殊的逆转录酶,它能在体内延伸染色体单链末端,在体外延伸寡聚核苷酸。一般认为端粒酶的蛋白催化亚基和RNA亚基组成了核心酶,它们对体外活性是必需的。
Telomerase is a specialized reverse transcriptase that elongates the single-stranded chromosome ends and oligonucleotides in vivo and in vitro .
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一般认为端粒酶的蛋白催化亚基和RNA亚基组成了核心酶,它们对体外活性是必需的。
It is believed that telomerase catalytic subunit and RNA subunit constitute the core enzyme , which is essential for the telomerase activity in vitro .
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4嫁接黄瓜叶片生长发育过程中SOD、POD和CAT组成的保护酶活性均明显高于自根黄瓜,MDA含量始终低于自根黄瓜。
The grafted cucumber had a higher SOD 、 CAT and POD activity and lower MDA content than the own root cucumber during the development .
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由SDS-PAGE和梯度PAGE测得全酶由4个亚基组成,全酶相对分子质量为360000,亚基相对分子质量为100000。
The molecular weight of the subunit and the whole enzyme were estimated by SDS-PAGE and gradation PAGE as 100 000 and 360 000 , respectively .
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木聚糖酶原酶与纤维素酶原酶组成的混合酶、木聚糖酶原酶与纤维素酶B组分组成的混合酶、木聚糖酶原酶与纤维素酶C组分组成的混合酶对纸浆纤维的损伤程度几乎相同。
The damage of pulp fibers by the enzyme mixture of crude xylanase and B or C fractions of cellulase was as same degree as that by the enzyme mixture of crude xylanase and crude cellulase .
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考察了液体发酵面包串珠霉MC-1产纤维素酶的影响因素(培养基组成及产酶发酵条件)。
This strain was named as MC-1.The studies on liquid fermentation conditions for MC-1 in 250 ml flasks were carried out through the single factor experiments . The results indicated that the culture medium components and the culture conditions had a significant influence on cellulase-producing .
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日粮中谷物组成和复合酶添加对生长猪生产性能和养分消化率的影响
Effect of cereal grains composition and multi-enzyme Preparation Supplementation on performance and nutrients digestibility of growing pigs
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为研究对象,探讨饲料中添加植酸酶和非淀粉多糖酶对大黄鱼生长、体组成以及消化酶活性的影响。
Effects of non-starch polysaccharide enzyme added in low energy diet on the growth performance in piglets ;
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从元素组成来看,酶显示出与通常蛋白质一样的碳,氢,氮及硫的元素比例。
In their elementary composition , the enzymes show the usual proportion of C , H , N , and S found in proteins .
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这几位科学家发现,这些端粒和组成他们的酶对于细胞的生命起着非常重要的作用。
The scientists found that these caps and the enzyme they are made of plays a crucial role in the life of the cell .
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动物机体内的各种离子有着重要作用,如构成组织的组成成分、酶的激活剂,维持渗透压及酸碱平衡等。
Ions in animals are necessary for constituting components of tissues and enzymatic activating agent , maintaining osmotic pressure of body fluids and acid-base balance .
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同时,米老排防火林带与防火生土带相比,能明显改善土壤微生物数量与组成、土壤酶活性、土壤理化性状以及水源涵养功能等,具有较强的地力维持功效且经济效益显著。
Compare to the original raw-soil fire prevention road , the fire prevention forest belt not only improving amount and buildup of soil microbe and soil enzymatic active and physical and chemical soil properties , boosting up the function of waterhead self-restraint , but also posses notable economic benefit .
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该基因可能编码由235个氨基酸组成的谷胱甘肽转移酶(GST),将其与刊源性较高的4个GSTcDNA进行氨基酸同源性比较,表现出较高的保守性。
Compared the sequence of amino acids with the four GST cDNA which are higher homologous , it shows the relatively higher conservation .
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目的研究组成型一氧化氮合酶(cNOS)及诱导型一氧化氮合酶(iNOS)在红藻氨酸(KA)诱导癫痫发作中的变化及作用。
Objective To study the changes and roles of cNOS and iNOS in kainic acid ( KA ) induced epilepsy seizures .
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膜脂组成和膜结合酶活性与玉米幼苗抗冷性的关系
The Relationship Among Membrane Lipids Membrane Linked Enzymes and Maize Cold-Resistance
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组成型一氧化氮合酶的表达与胃癌的侵袭转移和预后的关系
Relationships between the expression of cNOS mRNA in gastric carcinomas and the invasion , metastasis and prognosis
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根据棉、麻纤维的结构、组成不同,探讨酶洗中纤维素酶的作用方式与途径,并以此简释酶洗给棉、麻织物带来高附加价值的性能。
In respect to their varied structural composition of cotton and ramie fibres , different cellulase effects in various forms are discussed .
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选育组成型的海因酶产生菌对于酶法生产手性氨基酸具有重要的应用价值,同时可为海因酶的基因表达调控研究提供模式菌。
Therefore , the selection of strains with constitutive hydantoinases expression is very significant for application and can provide model strains for research on the regulation of hydantoinases expression .
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预处理的目的是改变木质纤维素宏观和微观结构及其超微化学组成,从而提高酶水解效率,增加可发酵糖的产量。
The purpose of pretreatment is to alter the lignocellulose macroscopic and microscopic structure as well as its sub-microscopic chemical composition , and to improve the rate of enzyme hydrolysis for increasing yields of fermentable sugars .
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结果表明,强化大豆水解蛋白的氨基酸组成与强化混合酶解蛋白及酪蛋白的氨基酸组成相似,且更接近FAO/WHO氨基酸需要量模式。
It was found that the essential amino acids composition of enriched soybean hydrolytic protein was similar to those of enriched enzymolysis protein and casein and closer to the FAO / WHO pattern of amino acid requirements .