定向进化

DNA改组&定向进化的技术革命

DNA Shuffling & technological revolution of directed evolution

易错PCR法定向进化D-海因酶的初步研究

A Preliminary Study on the Directed Evolution of D-hydantoinase by Error-prone PCR

抗CD3改型单链抗体的分子定向进化分子含有单糖分子链的一类碳水化合物。

Any of a class of carbohydrates whose molecules contain chains of monosaccharide molecules .

最近几年，在过去常用的寡核苷酸介导的随机突变、易错PCR和DNA改组等方法的基础上又出现了一些新的定向进化方法。

In the past few years , many new protocols for directed evolution were developed on the basis of oligonucleotide - directed random mutation , error-prone PCR and DNA shuffling .

抗抗CD3ScFv单克隆抗体的制备及鉴定抗CD3改型单链抗体的分子定向进化

Production of monoclonal antibody against anti-CD3 scFv Molecular Directed Evolution of Reshaped Anti-CD3 Single Chain Fv Fragment

目前致突变菌株（mutatorstrain）的使用是蛋白质定向进化中所采用的一种体内分子生物学方法。

Nowadays the application of mutator strain is a in vivo method in molecular biology for directed evolution of proteins .

常用的研究方法有易错PCR，DNAshuffling，定点诱变等等；如姜世民等利用异源DNA同源重组体外定向进化大肠杆菌的p-半乳糖苷酶。

Commonly used research methods are error-prone PCR , DNA Shuffling and site-directed mutagenesis and so on ; such as Jiang Shimin maked heterologous DNA homologous recombination in vitro evolution of E. coli P-galactosidase .

采用DNAshuffling的体外定向进化技术，本研究建立了来源于BL21（DE3）的EGI突变体库。

This research adopted vitro directional evolution technology of DNA shuffling , we established EGI mutant library from BL21 ( DE3 ) .

接着，本文还利用DNA家族改组技术对ptr抗性基因及其同源基因进行定向进化，为有效提高普那霉素产量打下基础。

Furthermore , directed evolution of ptr resistant gene and its homologous were performed with DNA family shuffling method , which lay the foundation for increasing the yield of pristinamycin . 1 .

DNA重排是目前为止最简便、最有效的体外定向进化技术，可以对单一基因、质粒、代谢途径、部分甚至整个基因组进行改造。

DNA shuffling is a simple and efficient technology for directed molecular evolution By mimicking and accelerating the process of sexual recombination , it can dramatically . optimize the sequence of single gene , plasmid , partial and even the whole genome of some viruses .

用体外定向进化的方法对EPSPS合酶基因草苷膦抗性机制的研究

The in vitro directed evolution of EPSPS gene to identify of base pairs related to resistant to glyphosate

文中综述噬菌体、细胞表面、核糖体、mRNA等展示技术的原理和方法，以及其在酶定向进化中的应用。

In this article , we review recent studies on display technology , which include not only the principles and methods of phage display , cell-surface display , ribosome display and mRNA display , but also their application in aspect of directed evolution of enzymes .

β-葡聚糖酶的定向进化及热稳定性研究

Studies on the Directed Evolution and Thermostability of β - glucanase

酶分子体外定向进化的研究进展

Advances in Study on Directed Molecular Evolution in Vitro of Enzyme

异源宿主中蛋白质内含子剪接活性的定向进化

Improved Protein Splicing by Directed Evolution of Inteins in Heterogenous Host

酶体外定向进化（Ⅲ）展示技术及其应用

Directed Evolution of Enzymes in Vitro (ⅲ) Display Technology and Its Application

定向进化提高枯草芽孢杆菌脂肪酶的活力

Increasing Activity of Lipase from Bacillus subtilis by Directed Evolution

大肠杆菌碱性磷酸酶的体外定向进化研究

The In vitro Directed Evolution of E.coli Alkaline Phosphatase

α-淀粉酶原因的体外定向进化研究

Directed Evolution in Vitro of α - Amylase Gene

体外定向进化是蛋白质工程中一个非常有效的设计策略。

Directed evolution has been proven to be an effective method for proteins engineering .

定向进化人α型干扰素基因家族的研究

Study on the Directed Evolution of Interferon Family

枯草杆菌脂肪酶的基因克隆、定向进化与非水酶学研究

Cloning , Directed Evolution and Non - aqueous Enzymology of Lipase from Bacillus Subtilis

酶定向进化的研究策略

Strategies for Study on Directed Evolution of Enzyme

酶立体选择性的定向进化及其高通量筛选方法

Directed Evolution of Enantioselective Enzymes and High-throughput Screening

人α型干扰素家族定向进化文库的构建及细胞筛选

Construction and Screening of the Directed Evolution Library of Interferon - α Family Genes

定向进化及定点突变提高枯草芽孢杆菌脂肪酶的活力和立体选择性

Enhancement of Activity and Enantioselectivity of Bacillus Subtilis Lipases by Directed Evolution and Site-directed Mutagenesis

葡激酶体外定向进化的初步研究

Preliminary Study on Directed Evolution of Staphylokinase

应用全合成噬菌体抗体展示库技术实现抗膀胱癌单链抗体的定向进化

Toward Directed Evolution of Single-chain Antibodies Against Human Bladder Carcinoma Using Synthetic Phage Display Library

蛋白质内含子体内定向进化和切割、分段表达及体外剪接

Directed Evolution , Cleavage , Segregate Expression of Inteins in Vivo and Splicing in Vitro

酶体外定向进化（Ⅰ）突变基因文库构建技术及其新进展

Directional Evolution of Enzymes in Vitro (ⅰ) Technique for Generating Mutator Libraries and Its New Progress