米氏常数

并对该酶的pH稳定性、热稳定性、米氏常数等进行了研究。

The characteristics of the enzyme such as pH stabilization , heat stabilization and Michaelis constant were studied .

酶与抗体反应前后的表观米氏常数Km及反应速度没有明显变化，表明抗体是结合在酶的非活性部位，没有或很少影响酶的活性中心的构象。

The apparent Michaelis constant and reaction velocity of the enzyme did not change after treatment with antibody . It was shown that the antibody might combine with a non-active site of acetylcholinesterase without affecting the conformation of its active site .

目的通过壳聚糖酶固定化前后最适宜的反应温度、pH值、米氏常数等动力学性质的变化研究载体电荷极性、空间阻力对壳聚糖酶动力学性质的影响。

Objective : To explore the effect of interspace diffuse resistance and carrier 's electrical property on immobilized chitosanase 's kinetic property .

米氏常数Km值在三个品系间无显著差异（P0.05）。

However , there was no significant difference of Km value among three strains ( P 0.05 ) .

兔肌乳酸脱氢酶米氏常数〔Km（NADH）〕的测定

Measurement of Km ( NADH ) of LDH from Rabbit Muscle

找出了固定化漆树酶的最适温度和最适pH，测定了它的米氏常数，热稳定性和重复使用性，并且与天然漆树酶进行了比较。

The properties of the immobilized laccase such as optimum temperature and pH , Km , stability and reusability were studied and compared with those of the native laccase .

通过对葡萄糖氧化酶（GOD）的米氏常数Km值及衡量扩散控制作用的Y值的计算，从理论上说明了扩散限制膜的引入是提高葡萄糖传感器响应线性范围的一种有效方法。

By calculating the values of Km and Y , it was explained theoretically that the introduction of polyurethane diffusion limited membranes was an effective method for extending the linear range of glucose snesor .

通过红外、紫外光谱等手段进一步分析其组成，对其中CMC（羧甲基纤维素酶）组分对热及pH的稳定性进行了分析，并测定了其米氏常数。

And then the characteristics of cellulase were searched by the infrared spectrum and ultraviolet spectrum . The heat and pH stabilities of CMC were also analyzed , and its Km were measured .

确定了米氏常数、考察了酶的底物专一性，研究了金属离子和温度、pH对酶活力的影响，为建立酶法生产1，3&丙二醇提供了依据。

Furthermore , other properties of the two separated enzymes such as substrate specificity , cation effect , optimum temperature and pH are characterized . These works can contribute to establish an enzyme catalyze system for 1 , 3-propanediol production .

pH-stat法在测定酶促反应米氏常数中的应用

Application of pH-stat Method on Determination of K_m of Enzymatic Hydrolysis Reaction

菌株15E所分泌的碱性蛋白酶的分子量为49.0kDa，等电点为9.3，米氏常数Km为5.6×10~（-3）g／mL。

Strain 15E could secrete alkaline protease , whose molecular weight was 49.0 kDa , isoelectric point was 9.3 and Km value was 5.6 × 10 ~ ( - 3 ) g / mL.

目的：研究α-淀粉酶及其底物2-氯-4-硝基苯-麦芽三糖苷（CNP-G3）酶促反应过程中的激动剂、抑制剂、最适pH、米氏常数等。

Objective : To investigate the effects of agonist , inhibitor , optimum pH and km value in the enzyme-promoting course of reaction of amylase using 2-chloro-4-nitrophenyl-alpha-D-maltotrioside ( CNP-G3 ) as substrate .

SDS作为一种蛋白质变性剂，不仅能够使HRP变性失去部分活性，而且还能使HRP对底物OPD的米氏常数Km（OPD）和最大反应速率都比其在缓冲体系中小；

As a denaturant for protein , SDS not only makes the HRP lose its activity because of denaturalization , but also decrease the Michaelis constant and the maximum velocity of the reaction for the substrate OPD .

利用底物消除法，测定了该类药物在大鼠肝微粒体中的表观酶动力学参数：米氏常数Km、最大反应速率Vmax和固有清除率CLint。

By substrate depletion approach , the apparent enzyme kinetics parameters michaelis constant ( Km ), maximum velocity ( Vmax ), and the intrinsic clearance ( CLint ) were determined in rat liver microsomes .

求算了米氏常数和反应活化能；

The constant of Michaelis and the activation energy were calculated .

计算了该实验条件下酶反应的米氏常数。

The Michaelis constant of enzyme reaction at present condition was calculated .

实验结果显示，两种模式下的米氏常数有所差异；

There was slight difference between Michaelis constants resulted from the two heating modes .

一级反应网络法求单底物酶促反应的米氏常数

Determination of the Michaelis Constant of Single Substrate Enzyme catalysed Reaction by the First order Reaction Network Theory

目的：以米氏常数相差较大的两种尿酸酶为模型，考察用两个底物浓度下标定比活性确定酶动力学参数和筛选抑制剂的可靠性。

AIM : To investigate the reliability of a linear kinetic method based on calibrated specific activities of enzyme at two substrate concentrations in estimating apparent kinetic parameters and characterizing inhibitors by using two uricases with different K_m as models .