酶动力学

DNA具酯酶活性的酶动力学初步研究

Preliminary Studies of Enzyme Kinetics on Esterase Activity of DNA

用酶动力学和聚合酶链式反应（PCR）对大鼠心肌线粒体呼吸链酶复合物Ⅰ、Ⅳ活性的变化及线粒体DNA的缺失进行测定。

Animal experiments with enzyme kinetics and PCR ( polymerase chain reaction ) technique were conducted .

用聚合酶链反应（PCR）、酶动力学和生物发光技术进行检测。

Polymerase chain reaction ( PCR ), enzyme kinetics and bioluminescence techniques were used .

酶动力学实验显示蛋白酶K增加CYP27A1酶活性呈时间，蛋白酶K剂量及底物浓度依赖性。

Kinetic studies showed that the increased activity was time , proteinase K and substrate concentration dependent .

TEM型β-内酰胺酶动力学研究

Study on the kinetics of TEM type β - lactamases

方法：应用细胞培养技术、噻唑盐比色测定（MTT）和酶动力学方法。

Methods : Using cell culture technique , MTT and enzyme kinetics methods .

PingPongBiBi机制的非稳态酶动力学布尔函数图论研究

Studies on Non-Steady State Enzyme Kinetics of Ping Pong Bi Bi Mechanism

酶动力学法测定培养上清中抗酒石酸酸性磷酸酶（TRAP）的活性；

The tartrate resistant acid phosphatase ( TRAP ) activities in the medium were measured by enzyme kinetics .

酶动力学方法测定碱性磷酸酶（ALP）活性。

ALP activity was measured by modified enzyme dynamical method .

方法：原代培养牙髓成纤维细胞、牙周膜成纤维细胞，用MTT法测定细胞增殖，酶动力学方法测定ALP活性。

METHODS : Detection of the cell proliferation by MTT method and ALP expression by enzyme dynamics method .

不同处理对水蜜桃果实PG酶动力学影响的研究

Research on the Effects of Different Treatments on the Enzymatic Dynamics of PG Enzyme in Juicy Peach Fruit

MFC的理论基础包括工程热力学、酶动力学和传质学等。

The theoretical basis of MFCs involves engineering thermodynamics , enzyme kinetics and mass transfer , et al .

以SDS-PAGE法测定其分子量及紫外分光光度法测定其酶动力学参数。

The molecular weight of which was determined by SDS-PAGE and the enzyme kinetics parameters of β - lactamas were determined by ultraviolet spectrophotometry .

方法：采用MTT比色试验及酶动力学方法，测定PDL细胞的增殖和ALP活性。

METHODS : MTT method and enzyme dynamics method were used to observe the ability of proliferation and ALP expression of PDL cells .

第二章建立了用酶动力学和落射显微荧光成像技术检测单个中性粒细胞内过氧化物酶（PO）活性的新方法。

In chapter two , a new method for determination of enzyme activity in single cells based on enzyme kinetics with an epi-fluorescence microscopy was developed .

肺炎克雷伯氏菌AmpCβ-内酰胺酶动力学及抑酶效应的研究

Study of kinetics of AmpC β - lactamase in Klebsiella pneumoniae and the inhibitory activity of β - lactamase inhibitors

分别考察了pH、温度、金属离子、化学试剂对重组ADI活性的影响，同时考察了它的温度稳定性，测定了酶动力学参数。

The effects of pH , temperature , metal ions and chemicals on the activity of recombinant ADI were investigated , as well as its thermostability and kinetic parameters measurement .

主要工作包括：基于酶动力学理论、化学反应理论和传质学理论分析了MFC工作性能的影响因素。

The main work are summarized as follows : The factors affecting the MFC performances were analyzed from enzyme kinetics , chemical reaction theory and mass transfer .

描述了在批式反应器和连续流搅拌反应器（CSTR）中酶动力学拆分对映异构体的不同之处。

The difference between a continuous stirred tank reactor ( CSTR ) and a batch reactor was analyzed for the enzymatic kinetic resolution of enantiomers .

介绍了一种采用基于酶动力学分析法，以发光二级管（LED）作为单色光源，以电池供电的新型手持式有机磷和氨基甲酸酯类农药残留检测仪。

A new hand-held photometric analyzer for pesticide residue monitor based on the acetylcholinesterase ( AchE ) enzyme catalysis kinetic analysis method , light emitting diodes ( LED ) as monochromatic source and dry battery as power supply is presented in this paper .

本研究利用酶动力学方法测定了边茶萃取物对脂肪酸合酶（fattyacidsynthase，FAS，E.C.2.3.1.85）的抑制作用，近几年研究报道该酶是减肥和抑癌的双重潜在靶点。

The inhibition on Fatty acid synthase ( E.C.2.1.3.85 , FAS ) by the extracts of brick tea is measured by inhibition kinetics . FAS has been reported as a potential new therapeutic target for both cancer and obesity .

血清醌还原酶（QR）、ALT、LDH和CK催化活性用酶动力学方法测定，肝丙二醛（MDA）含量按硫代巴比妥酸方法测定。

Serum quinone reductase ( QR ), ALT , LDH and CK activities were determined with enzyme dynamic methods . Liver malondialdehyde ( MDA ) content was measured by the method of thiobarbituric acid .

实时定量聚合酶链反应（real-timeQuantitativePCR，RQ-PCR）是综合PCR、分子杂交和酶动力学的一种新技术，它根据荧光共振能量转移（fluorescenceresonanceenergytranslation，FRET）的原理进行。

Real-time quantitative polymerase chain reaction ( RQ-PCR ) is a new technology , which develops from PCR ? hybridization and enzymic technology , and it is base on the principle of the fluorescence resonance energy translation .

方法应用全自动生化酶动力学法和RIA法检测DM组和对照组血清中空腹血糖（GLU）、胆固醇（TCh）、甘油三脂（TG）、胰岛素（Ins）的含量。

Methods All-automatic biochemical enzyme kinetic and RIA were applied to detect the contents of fasting blood-glucose , TCh , TG and Ins in plasma in the DM group and control group .

在10ng/ml的IL-1β作用4d后用ELISA法检测纤维连接蛋白（Fn）的含量，用酶动力学方法检测碱性磷酸酶（ALP）活性。

Fibronectin level in the medium was determined by ELISA at the fourth days after 10ng / ml IL-1 β treatment , and alkaline phosphatase ( ALP ) activity was measured by enzyme kinetic method .

其次，我们在NK的成熟肽上，挑选了两个与前导肽有氢键作用的氨基酸残基，分别进行定点突变之后，分析结构信息和酶动力学信息，同时，我们用生物信息学进行辅助研究。

Secondly , we selected two amino acid residues in mature peptide to perform site-directed mutagenesis , and analyzed the structure information and enzyme kinetics . At the same time , we used bioinformatics as supporting research .

方法采用酶动力学方法分别在30d、60d、120d、240d、360d测定四种酶活力，并计算酶的保活率（%）。

Methods The activities of four kinds of enzymes were measured with kinetic at 30 d. 60 d. 120 d.

采用同样条件进行二次酶动力学拆分，得到ee值>98%的手性烯酮醇，总拆分收率23%。

The chiral cyclopentenone of > 98 % enantiomer excess was obtained by a second time enzymic kinetic resolution under the same conditions , and the total yield of the enzymic resolution was 23 % .

结论：此线性动力学方法仅用高于Km的底物浓度也能可靠确定酶动力学参数，有利于用常规定量方法筛选对底物具有高亲合力特殊靶酶的抑制剂。

CONCLUSION : This linear kinetic method , reliable in estimating kinetic parameters and characterizing inhibitors of enzyme with lower K_m by using two concentrations of the substrate above its K_m , makes it possible to use routine technique for quantification .

方法：酶动力学方法测定30例白血病患者血清LDH及HBDH活性，并对急性期与缓解期酶活性作对比分析。

Methods Serum LDH and HBDH were analysed in 30 Cases untreated leukemia patients by enzyme dynamics and the enzyme activity of the serum LDH and HBDH in the acute and remission period were compared .