酶活

从牛粪中分离到一株降解纤维素的真菌ZJ（Trichoderma.sp），对其进行了FPA酶活、CMC酶活的测定以及固态发酵试验。

A cellulolytic mould fungus of ZJ ( Trichoderma . sp ) was isolated from cattle feces , for which FPA and CMC enzyme activity test and solid-state fermentation were carried out .

酒精中毒小鼠全血SOD酶活测定

Determination of SOD Enzyme Activity in the Blood of Rats Suffering from Alcoholism

棉花叶片组织中GO活性、GO蛋白含量和内源H2O2含量均成正相关，酶活高的叶片GO蛋白含量高、H2O2含量也高。

GO activity positively correlated with GO protein and H2O2 level .

4株南极嗜冷菌分泌的蛋白酶的酶活反应最适pH值为9。

The maximum proteolytic activity occurred at pH 9 for the four Antarctic psychrotrophic bacteria .

Cd胁迫对水稻根际土壤酶活和微生物的影响

Effects of Cadmium Stress on Microbes and Enzyme Activity in Rice Rhizosphere Soil

DNA拓扑异构酶抑制剂的筛选及两种化合物的酶活抑制机制、抗肿瘤活性研究

The Screening of DNA Topoismerase Inhibitor and Inhibiting DNA Topoismerase Mechanisms and Antineoplasmic Activity of Two Chemical Compounds ; grain drive shifter

叠氮钠（NaN3）对ELISA检测酶活影响的探讨

Approach of sodium azide affecting enzyme activity in ELISA

结果表明CAT酶活随着超声功率和超声时间增大而降低。

It was showed that the catalytic activity of CAT was decreased with increasing ultrasound power and time .

采用3，5&二硝基水杨酸法（DNS法）来测定酶活和木聚糖含量。

It determined enzyme activity and sugar content through DNS method .

与游离细胞相比较，固定化细胞酶活随pH变化的范围以及对温度适应的范围大致与游离细胞相仿。

The optimum pH range and the optimum temperature of the immobilized cells were as same as that of the free cells .

而CMC酶活分别为160、210；

Those CMC enzyme activity attained 160 , 210 respectively ;

GP的乙酰化和酶活受细胞内能量状态的调控。

Cell energy status can regulates the acetylation and activity of GP .

筛选获得了高产SOD大蒜悬浮细胞系，培养18d后达到最大生物量及最大SOD总酶活分别为22.12g。

High yield SOD garlic suspension cell line was obtained after selection .

用NorthernBlot，westernblot和酶活测定的方法检测了转基因水稻中几丁质酶和葡聚糖酶的表达和活性。

The expression level and enzymic activity of transgenic rice were evaluated by Northern blot , Western blot and the enzyme reaction .

进一步分析了pH和温度对酶活的影响发现，该酶最适pH为8.0，最适温度为25℃。

Determination of effect of pH and temperature on enzyme activity shows that the optimal reaction condition of pH was 8.0 and temperature was 25 ℃ .

EDTA对酶活具有部分抑制作用。

EDTA has partially inhibition action .

酶活测定证明该蛋白48h的发酵上清具有碱性磷酸酶活性，酶活性约为18.79金氏单位。

The 48h zymogenic supernatant had alkaline phosphatase activity , the activities was 18.79 .

使诱导了PAL酶活的改变和酚的积累等次级代谢反应。

Enzyme and phenolic accumulation .

倒数第二位叶片超微弱发光与CAT、POD、AP酶活成正相关；与蛋白含量成负相关。

Last but one blade has positive correlation with CAT , POD and AP activity , and has negative correlation with protein level .

以L-抗坏血酸（Vc）为终止剂改进的经典胆红素氧化酶（BOD）酶活测定方法。

The normal method for the measurement of bilirubin oxidase was improved by using ascorbic acid as the terminating agent .

钼酸胺法测定试验样品植酸酶酶活为977U／mL。

The lyse of the transfected DEF was used to test the activity of phytase by the ammonium molybdic method and it reached to 977 U / mL.

对小麦SOD活性的研究表明，小麦叶片中POD酶活的上升可能与其它途径而不是SOD诱导产生的H2O2有关。

It is indicated that the increase of POD activity in the leaves may be caused by H_2O_2 produced from sources other than SOD .

单因子实验发现，0.5%聚乙二醇6000对PAL酶活的影响最大，可提高活力80.3%。

By single factor experiment , 0.5 % PEG was found to significantly improve PAL activity by 80.3 % .

但从萌发第1d开始酶活就受到抑制，均低于对照。

However , a-amylase activities were suppressed from the first day of germination , lower than the control .

通过CMC酶活和滤纸酶活测定，结果显示酶活情况都很好。

The results showed that enzyme activity is good to get through enzyme activity determination of the CMC and filter paper .

重组Pichia酵母（Mut~S）发酵过渡阶段关键酶活分析

Analysis of Key Enzymes in Metabolic Pathways of Recombinant Pichia pastoris ( Mut ~ S ) at Transition Phase

在以上获得的培养基和培养条件下培养48h后测酶活，脱卤酶活力达到8.76U/g干菌体，比在原始条件下提高约10倍。

Under the optimal conditions dehalogenase activity reached 8.76 U / g and enhancing 10 times for 48 h.

培养基最佳组合为淀粉30g/L、蛋白胨35g/L和酵母膏3g/L。添加硝酸钠有利于MTG的积累，酶活比对照提高了31%。

MTG activity is improved by 31 % by adding sodium nitrate .

SDS-PAGE与westernblot分析证明重组ADI基因获得了表达，酶活测定证明其具有一定活性。

The expression of recombinant ADI gene was demonstrated through SDS-PAGE and Western blot analysis , and its enzymatic activity was later confirmed by activity assay .

生物酸化技术应用于啤酒生产可降低糖化醪pH值，实现对麦芽、糖化醪和麦芽汁的酸化，生物酸化可增加酶活。

The application of biological acidification in beer brewing could effectively reduce pH value of saccharifying mash , achieve the acidification of malt , saccharifying mash , and wort , and increase enzyme activities .