稳定遗传

高静水压处理水稻诱导稳定遗传变异系的DNA分析

Molecular Analysis of Stable Mutagenesis Rice Cultivar Induced by High Hydrostatic Pressure

农杆菌介导的水稻转化及bar基因稳定遗传

Agrobacterium-mediated transformation of rice and stable inheritance of bar gene

根癌农杆菌介导的基因转化过程中，TDNA的整合是关系到外源基因能否稳定遗传的关键步骤。

The T-DNA integration is a critical step of the steady inheritance for foreign genes during the Agrobacterium tumefaciens-mediated transformation .

DNA分子标记是指以DNA的多态性为基础的遗传标记，能够稳定遗传，且遗传方式简单。

DNA molecular markers , based on the polymorphisms at the DNA level , were generally stably inherited in a simple mode .

稳定遗传的CMS系中有极少量可育株的出现；

Farthing fertile plants were found in the CMS lines ;

研究结果表明：高静水压可以对高等植物的种子进行诱变，产生在表型上及DNA分子水平上均可稳定遗传的变异。

This is the first time we have shown at molecular level that treatment by high hydrostatic pressure can induce genetically stable mutations in higher plants .

目的：建立可稳定遗传、临床症状显著的凝血因子（F）基因剔除小鼠近交系。

Objective : To establish a inbred strain of the coagulation factor ⅸ knockout mice with stable heredity and obvious clinical symptoms .

荧光筛选获得生殖系稳定遗传的F1代胰岛β细胞转基因斑马鱼。

Fluorescence screening obtained a stable heredity F1 generation of beta cell transgenic zebrafish .

转基因小鼠中人清道夫受体AI基因的稳定遗传和特异表达

Genetics Stability and Expression Specificity of Human Scavenger Receptor - A I on Endothelial Cells in Transgenic Mice

非DNA序列变异，如DNA甲基化、组蛋白修饰等亦可改变生物性状，包括重要农艺性状如株高、花期、抗性、产量等，并可稳定遗传。

Non-DNA sequence variations , such as DNA methylation and histone modification , can also change biological traits such as plant height , flowering time , resistance and yield .

转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究

Stable Heredity and Efficient Expression of Bt Insecticidal Protein Gene in the Transgenic Rapeseed and Its Insect-resistant Activity

组织化学和SDS-PAGE检测结果表明，外源基因能够稳定遗传，功能得到正确表达。

Results of the histochemical and SDS-PAGE analysis showed that the target genes were stably inherited .

人G-CSF转基因鼠的稳定遗传与表达

Human G CSF Transgene Stable Transmission and Expression in Mice

PCR和Southern杂交证实NP-1基因已经稳定遗传至部分T1代玉米植株中。

Genomic PCR , Southern blotting results revealed that NP-1 gene was transmitted stably to T1 generation .

大肠杆菌转化是将外源DNA引入大肠杆菌中，从而改变大肠杆菌的性状，并稳定遗传下去。

Escherichia coli ( E. coli ) transformation is defined as the introduction of foreign DNA into E. coli which will chang the characteristics of E. coli , and can be steadily passed on .

通过对转化子的PCR检测和Southern杂交分析表明，TDNA已整合进毛壳菌基因组中，而且在所检测的转化子中都是以单拷贝的形式整合，转化子都能够稳定遗传。

PCR and Southern analysis showed that the T-DNA was integrated into the genome . Among of the examined transformants contained a single copy , and was stable through mitotic cell division .

T1代植株PCR检测结果表明，Bt基因能够在转基因后代中稳定遗传，基因分离符合1∶1的孟德尔遗传分离规律。

The molecular detection of T1 progeny proved that mostly transgenic plant could inherit steadily and their segregation pattern fell into Mendel mode 1 ∶ 1 .

nit突变特性在自交和无性繁殖过程中能够稳定遗传；

The nit mutation could be steadily inherited by self-cross process and asexual reproduction .

将D1代获得5株阳性转化植株的种子在温箱中发芽，提取DNA检测，得到D2代稳定遗传的阳性转化植株2株。

The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested . Two stable positive inversion plants were obtained in D2 generations .

结果表明：T3代经PCR及PCR-Southern杂交鉴定，目的基因已经整合到小麦的基因组中，并能在小麦基因组内稳定遗传；

By PCR and PCR-Southern analysis , anti-PLD γ gene had been integrated into wheat genomes of T_3 generation with genetic stability .

选择部分T1代潮霉素抗性植株进行Southern杂交鉴定，结果证实，外源基因已经稳定遗传给T1代。

Several lines showing resistance to hygromycin B were also analyzed with Southern blotting . The results further confirmed that ThEn-42 gene could stably inherit to T1 generation .

IPT基因在不同遗传背景的F1组合、不同回交世代材料中能稳定遗传，符合孟德尔遗传定律。

IPT gene could inherit stably in FI combination derived from different background and in different backcross generation , which fitted to the law of Mendel .

通过稳定性检测，发现融合子13-2的潮霉素抗性能稳定遗传，且液态发酵monacolink水平稳定性良好。

The stability testing found that , hygromycin resistance gene could be inherited stably , and the level of Monacolin K production in liquid state fermentation was stable .

对部分T2代株系进行潮霉素抗性鉴定和PCR鉴定，结果表明ThEn-42已经稳定遗传给T2代植株。

The PCR analysis and hygromycin B resistant analysis of some T2 plants also demonstrated that ThEn-42 gene had stably inherited to T2 generation . 2 .

转基因水稻中ThEn-42基因的稳定遗传及其抗病性的提高

Genetic Stability and Enhanced Resistance of Transgenic Rice with ThEn-42 Gene

为进一步育种研究，于SPF级动物房内进行近交化培育保种，其突变表型可稳定遗传。

Now they are raised in the SPF level animal room . During the breeding , we found that the mutant phenotype of the KM mutant mice can inherit steadily .

通过PCR及PCR-Southern杂交，证实了NP-1基因已经整合到转基因植株的基因组中，并稳定遗传到T1。

Genomic PCR and PCR-Southern revealed that NP-1 gene was integrated into the genome of these transgenic plants , and it was transmitted to T1 generation stably .

Mtr性状在无性后代稳定遗传，在游动孢子后代连续三代未发生抗药性分离。

The resistance to metalaxyl of Mtr isolate was inherited stably , and they did not show variation from the first zoospore progeny to the third zoospore progeny .

反义Wx基因在转基因水稻后代中是可以稳定遗传的，并可按正常方式表达，直链淀粉含量可在转基因水稻不同世代间保持一致。

Genetic analysis demonstrated that the antisense Wx gene and improved amylose content are stably inherited ( up to ninth generation ) in these transgenic lines .

84K杨叶片不定芽直接分化系统的建立，为84K杨基因转化提供了一种良好的受体系统，并为外源基因的稳定遗传提供基础。

It offered an favourable plant transformation passive system and a steady foundation of genetic transformation .