着丝粒序列

本研究利用显微切割技术及PCR扩增技术，探寻棉花的着丝粒序列，主要研究结果如下：1.棉花染色体或染色体片段微分离及LA-PCR技术体系的建立。

In this study , we used microdissection and PCR technology , explore the cotton centromere . The main results were as follows : 1 . Establishment of chromosome or chromosome segment microdissection and LA-PCR technology in cotton .

黑麦B染色体着丝粒表达序列的克隆和分析

Cloning and characterization of a centromere express sequence of rye B chromosome

水稻第四号染色体着丝粒的完整序列是高等生物中完成的第一条染色体着丝粒序列，为研究着丝粒的功能提供了结构基础，也为构建植物或者水稻人工染色体提供了必需的序列信息。

The complete sequence of rice chromosome 4 is the first centromere sequence in higher eukaryotic species . The structural information may shed some light on the functional dissection of a centromere and it is also useful for constructing rice or plant artificial chromosomes .

在物理图构建的过程中鉴定了覆盖水稻第四号染色体着丝粒的重叠群，并对这个重叠群进行了测序，获得了第一个完整的水稻染色体着丝粒序列结构。

We reported the sequence structures of the first complete rice chromosome centromere through identifying and sequencing a large BAC clone-based contig , which covered the rice chromosome 4 centromere .