流式细胞仪

以流式细胞仪测定其DNA指数值；

DNA index ( DI ) were determined by flow cytometer ( FCM ) .

流式细胞仪分析DC表型；

The phenotype of DC was analyzed by flow cytometer .

应用流式细胞仪对13例肾母细胞瘤DNA倍体进行了分析。

Tumor cell DNA analysis in 13 cases of nephroblastomas were studied by flow cytometry .

提要本文报告用流式细胞仪测定16例不同癫痫病灶中DNA含量和细胞周期的变化情况。

The DNA contents and cells periods in16 resected epileptic focuses were determined by Flow Cytometric Analysis .

方法：采用直接荧光法，通过流式细胞仪分析人外周血T淋巴细胞亚群、B淋巴细胞数量。

Method T and B lymphocyte were determined by immunofluorescence assay through fluorescence activated cell sorter ( FACS ) .

流式细胞仪分析出现低于G1期DNA含量的亚二倍体凋亡率。

Sub-G1 cells were observed by flow cytometry , the total sub-G1-phase .

胸腺大鼠胸腺细胞体外培养条件下，加入H2O2和不同剂量的GPC，用流式细胞仪术分析GPC对H2O2引发的胸腺细胞凋亡及线粒体膜电位的影响作用。

Thymus cell : Thymus cells of mouse were cultured in vitro .

琼脂糖凝胶电泳观察DNA降解，以及应用流式细胞仪观察细胞凋亡过程中细胞周期的变化。

DNA ladder and cell cycle were examined by DNA agarose gel eletronphores and PI fluorescence flow cytometry ( FCM ), respectively .

使用的实验方法为荧光标记后激光共聚焦成像、流式细胞仪检测和westernblot。

Western Blot , flow cytometry and fluorescently confocal imaging after fluorescent labeling were used in experiment . 5 .

利用流式细胞仪以及realtimeRT-PCR检测细胞的凋亡率和凋亡基因的表达量，以及类固醇相关合成酶的表达量。

The apoptosis rate of GCs and the expression of apoptosis-related genes and steroid synthesis-related genes were detected by FCM and real time RT-PCR respectively .

结果：流式细胞仪检测40例恶性胸腹水细胞DNA非二倍体出现率为90%（36/40），脱落细胞学的检出率为875%（35/40）。

Results : The detection rate of DNA non-diploid is 90 % by FCM and 87.5 % by the method of exfoliative cytology .

利用流式细胞仪检测人外周血NK细胞毒性方法的建立

Measuring human peripheral NK cell cytotoxicity with flow cytometry

其检测方法有HE染色法、免疫组化染色法、PCR以及Rt-PCR技术，流式细胞仪技术。

The detection methods of micrometastasis include HE staining , immunohistochemical staining , PCR , Rt PCR technique .

流式细胞仪分析表明，在HL-60细胞分化凋亡过程中bcl-2表达逐渐下降，凋亡细胞bcl-2表达水平较低，而未凋亡细胞bcl-2仍维持在高水平。

Flow cytometric analysis revealed that bcl-2 oncoprotein was down-regulated during differentiation and apoptosis process .

通过流式细胞仪检测PI染色的细胞DNA，发现病毒感染组出现明显凋亡峰；

Flow cytometric observation on DNA of PI stained cells showed obviously an apoptotic peak in the Cox B infected group .

流式细胞仪检测凋亡峰及bax，bcl-2等凋亡相关基因的表达。

The apoptosis and the expression-associated genes peaks were detected with flow cytometry ( FCM ) .

流式细胞仪检测MHCⅠ类分子表达。

MHC class ⅰ molecule expression was measured by FACS assays .

采用流式细胞仪（FCM），观察额叶皮质、海马区神经细胞凋亡时程变化情况。

The apoptosis was examined by the technique of flow cytometry ( FCM ) .

CD3单抗诱导小鼠胸腺细胞凋亡的流式细胞仪检测

Detection of mouse thymocytes apoptosis induced with CD3 McAb by flow cytometer

化学荧光法检测Bcl-2的细胞表达；电镜观察、流式细胞仪与原位末端标记方法检测细胞凋亡情况。

Bcl-2 expression by fluoresce and cell apoptosis by electronic microscopy , flow cytometry and in situ terminal staining .

方法MTT法，AO/EB双荧光染色法，琼脂糖凝胶电泳，流式细胞仪方法。

METHODS MTT assay , AO / EB double staining , agarose gel electrophoresis , flow cytometry .

晚期喉癌切缘组织流式细胞仪参数及PCNA表达的研究

Changes of flow cytometry parameters and PCNA expression in the adjacent tissues of advanced laryngeal carcinoma

采用四聚体技术，运用流式细胞仪检测病毒特异性CTL。

Flow cytometry was used to exam viral specific CTL .

流式细胞仪测定姜黄素处理后MCF-7细胞周期分布变化与凋亡量；

FCM was accepted for detecting of cell cycle distribution and apoptosis .

流式细胞仪DNA含量分析：观察NS398对Pc一细胞周期及凋亡的影响。

FCM Assay for analysis of cell cycle .

流式细胞仪分析发现在G1峰前出现一个亚二倍体峰即凋亡峰；

A apoptotic peak appeared on flow cyto metry ;

以上各组分别于培养24h后，收集细胞，用流式细胞仪检测细胞周期。

After 24h incubation , cell cycle was measured by flow cytometry ( FCM ) .

应用流式细胞仪进行细胞周期分析，并通过体外核转录实验测定总RNA聚合酶活性和RNA聚合酶Ⅱ活性。

And total RNA polymerases activity and RNA polymerase ⅱ activity were measured in isolated HL-60 nuclei by nuclear transcription in vitro .

分别于1、4及12周时取静脉血，采用流式细胞仪分别计数各组大鼠外周血EPC数量的变化。

Flow cytometry was used to identify and count the number of EPC from peripheral blood .

流式细胞仪测定单克隆抗体识别福氏2a痢疾杆菌及其突变株表面抗原分子表达的分析

Preliminary Analysis of Surface Antigen Expression on Shigella flexneri 2a using Flow Cytometry